Crystallization (Comment) | Organism |
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crystals are grown by using the hanging drop vapor diffusion method with seeding.1.5 A resolution the X-ray crystal structure of the complex of BeF3 2 with phosphoserine phosphatase. The structure is comparable to that of a phosphoenzyme intermediate: BeF3- is bound to Asp11 with the tetrahedral geometry of a phosphoryl group, is coordinated to Mg2+, and is bound to residues surrounding the active site that are conserved in the haloacid dehalogenase (HAD) superfamily | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
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BeF3- | at 50°C, the hydrolytic activity of is markedly inhibited (more than 95%) in the presence of both BeCl2 and NaF. The latter is provided at concentrations that yield predominantly BeF3- in situ. Either BeCl2 or NaF alone has a much smaller inhibitory effect (35 or 20%, respectively). Similar inhibition by BeF3- is observed at room temperature or at 70°C | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
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Methanocaldococcus jannaschii | Q58989 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58989 | - |
- |
Synonyms | Comment | Organism |
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PSP | - |
Methanocaldococcus jannaschii |