Crystallization (Comment) | Organism |
---|---|
structures of complexes with L-histidinol 1-phosphate, L-histidinol, and phosphate as well as the structure showing the cross-linking between two enzyme molecules. The enzymatic reaction requires Mg2+ cations and is catalyzed mainly by amino acid residues from the N-terminal domain. The C-terminal domain is responsible for the substrate specificity | Medicago truncatula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO2 | enzyme dimers in the presence of CO2 or formaldehyde form mutual, methylene-bridged cross-links between Lys158 and Cys245 residues | Medicago truncatula | |
formaldehyde | enzyme dimers in the presence of CO2 or formaldehyde form mutual, methylene-bridged cross-links between Lys158 and Cys245 residues | Medicago truncatula | |
Li+ | - |
Medicago truncatula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.263 | - |
L-histidinol phosphate | pH 8.4, 23°C | Medicago truncatula |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimum concentration 5 mM | Medicago truncatula |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | G7J7Q5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a N-terminal signal peptide. The cleavage is predicted to occur between Arg51 and Ala52 | Medicago truncatula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidinol phosphate + H2O | - |
Medicago truncatula | L-histidinol + phosphate | - |
? | |
additional information | no substrate: D-myo-inositol-1-phosphate | Medicago truncatula | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
11419457 | - |
Medicago truncatula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
L-histidinol phosphate | pH 8.4, 23°C | Medicago truncatula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Medicago truncatula |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
5.5 | - |
pH 8.4, 23°C | Medicago truncatula | Li+ |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.7 | - |
L-histidinol phosphate | pH 8.4, 23°C | Medicago truncatula |