Cloned (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
expressed in Escherichia coli | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
C150S | insensitive toward inactivation with oxygen or thioredoxin, no effect on activity | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | activity of the native enzyme but not the C150S mutant can be completely abolished with oxygen, reversible with thiol-containing compounds | Archaeoglobus fulgidus | |
thioredoxin | inhibits native enzyme but nut C150S mutant | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
4-nitrophenyl phosphate | native enzyme, pH 8.0, 80°C | Archaeoglobus fulgidus | |
4.1 | - |
4-nitrophenyl phosphate | C150S mutant enzyme, pH 8.0, 80°C | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000 | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol 1-phosphate + H2O | Methanocaldococcus jannaschii | - |
myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | Archaeoglobus fulgidus | - |
myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | Methanocaldococcus jannaschii MJ0109 | - |
myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | Archaeoglobus fulgidus AF2372 | - |
myo-inositol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Archaeoglobus fulgidus AF2372 | - |
- |
- |
Methanocaldococcus jannaschii | - |
- |
- |
Methanocaldococcus jannaschii MJ0109 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Archaeoglobus fulgidus AF2372 | 4-nitrophenol + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | - |
Methanocaldococcus jannaschii | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | - |
Archaeoglobus fulgidus | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | - |
Methanocaldococcus jannaschii MJ0109 | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | - |
Archaeoglobus fulgidus AF2372 | D-fructose 6-phosphate + phosphate | - |
? | |
additional information | archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity | Archaeoglobus fulgidus | ? | - |
? | |
additional information | archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity | Archaeoglobus fulgidus AF2372 | ? | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii MJ0109 | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus AF2372 | myo-inositol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000 | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
More | archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity | Methanocaldococcus jannaschii |