BRENDA - Enzyme Database
show all sequences of 3.1.3.23

Identification of a new phosphatase enzyme potentially involved in the sugar phosphate stress response in Pseudomonas fluorescens

Maleki, S.; Hrudikova, R.; Zotchev, S.; Ertesvag, H.; Appl. Environ. Microbiol. 83, e02361 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pseudomonas fluorescens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Mn2+
addition of MN2+ decreaseds the reaction rate both without and in combination with Mg2x05
Pseudomonas fluorescens
NaCl
50 mM, inhibitory
Pseudomonas fluorescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
D-fructose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
1.8
-
D-glucose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
optimum concentration 2.5 mM
Pseudomonas fluorescens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas fluorescens
C3K9U8
-
-
Pseudomonas fluorescens SBW25
C3K9U8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + H2O
-
749666
Pseudomonas fluorescens
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
-
749666
Pseudomonas fluorescens SBW25
4-nitrophenol + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
749666
Pseudomonas fluorescens
D-fructose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
749666
Pseudomonas fluorescens SBW25
D-fructose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
-
749666
Pseudomonas fluorescens
D-glucose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
-
749666
Pseudomonas fluorescens SBW25
D-glucose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
preferred substrate
749666
Pseudomonas fluorescens
D-ribose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
preferred substrate
749666
Pseudomonas fluorescens SBW25
D-ribose + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 29900, calculated from sequence and SDS-PAGE, recombinant protein including His-tag
Pseudomonas fluorescens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.6
-
D-fructose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
11.4
-
D-glucose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas fluorescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Mn2+
addition of MN2+ decreaseds the reaction rate both without and in combination with Mg2x05
Pseudomonas fluorescens
NaCl
50 mM, inhibitory
Pseudomonas fluorescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
D-fructose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
1.8
-
D-glucose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
optimum concentration 2.5 mM
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + H2O
-
749666
Pseudomonas fluorescens
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
-
749666
Pseudomonas fluorescens SBW25
4-nitrophenol + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
749666
Pseudomonas fluorescens
D-fructose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
749666
Pseudomonas fluorescens SBW25
D-fructose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
-
749666
Pseudomonas fluorescens
D-glucose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
-
749666
Pseudomonas fluorescens SBW25
D-glucose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
preferred substrate
749666
Pseudomonas fluorescens
D-ribose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
preferred substrate
749666
Pseudomonas fluorescens SBW25
D-ribose + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 29900, calculated from sequence and SDS-PAGE, recombinant protein including His-tag
Pseudomonas fluorescens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9.6
-
D-fructose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
11.4
-
D-glucose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
General Information
General Information
Commentary
Organism
physiological function
deletion of the Spp gene does not affect growth of the wild-type strain on fructose, but the gene cannot be deleted in the alginate-producing strain. Sugar phosphate accumulation in Spp mutant strains causes diminished growth in some of the strains, which is partially relieved by Spp overexpression. Overexpression of Spp in fructose-grown alginate-producing strains negatively affects both growth and alginate production
Pseudomonas fluorescens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
deletion of the Spp gene does not affect growth of the wild-type strain on fructose, but the gene cannot be deleted in the alginate-producing strain. Sugar phosphate accumulation in Spp mutant strains causes diminished growth in some of the strains, which is partially relieved by Spp overexpression. Overexpression of Spp in fructose-grown alginate-producing strains negatively affects both growth and alginate production
Pseudomonas fluorescens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.4
-
D-glucose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
13
-
D-fructose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.4
-
D-glucose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
13
-
D-fructose 6-phosphate
pH 7.5, 23C
Pseudomonas fluorescens
Other publictions for EC 3.1.3.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750977
Zeng
Essential roles of the sppRA ...
Streptococcus mutans
J. Bacteriol.
201
e00586
2019
-
-
1
-
-
-
1
2
-
2
-
-
-
1
-
-
-
-
-
-
-
1
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
-
2
-
-
-
-
-
-
-
-
-
1
3
1
-
-
-
-
1
-
-
-
2
1
1
2
-
-
749666
Maleki
Identification of a new phosp ...
Pseudomonas fluorescens, Pseudomonas fluorescens SBW25
Appl. Environ. Microbiol.
83
e02361
2017
-
-
1
-
-
-
2
2
-
1
-
-
-
5
-
-
-
-
-
-
-
-
8
1
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
1
-
-
-
-
-
-
-
-
-
-
8
1
-
-
-
2
-
-
-
-
-
1
1
-
2
2
730458
Guggisberg
A sugar phosphatase regulates ...
Plasmodium falciparum
Nat. Commun.
5
4467
2014
-
-
1
1
1
-
-
-
1
1
1
1
-
3
-
-
1
-
-
-
-
-
16
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
1
1
1
1
-
-
-
1
-
-
-
-
16
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
730667
Caparros-Martin
The kinetic analysis of the su ...
Arabidopsis thaliana
Planta
240
479-487
2014
-
-
1
-
5
-
-
22
-
1
1
-
-
2
-
-
1
-
-
-
-
-
8
1
-
-
-
17
1
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
22
-
1
1
-
-
-
-
1
-
-
-
-
8
1
-
-
-
17
1
-
-
-
-
-
-
-
23
23
714982
Godinho
Characterization and regulatio ...
Bacillus subtilis
FEBS J.
278
2511-2524
2011
-
-
1
-
3
-
-
7
-
1
1
1
-
3
-
-
1
-
-
-
-
-
8
1
1
1
-
7
1
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
7
-
1
1
1
-
-
-
1
-
-
-
-
8
1
1
1
-
7
1
1
-
-
-
2
2
-
7
7
94728
Ye
-
Inducer expulsion and the occu ...
Enterococcus faecalis, Streptococcus pyogenes
Microbiology
142
585-592
1996
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94725
Cook
Properties of two sugar phosph ...
Streptococcus equinus
J. Bacteriol.
177
7007-7009
1995
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94727
Ye
Purification and characterizat ...
Lactococcus lactis
J. Biol. Chem.
270
16740-16744
1995
1
-
-
-
-
-
-
1
1
5
-
-
-
1
-
-
-
-
-
-
-
-
10
-
-
-
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
5
-
-
-
-
-
-
-
-
-
-
10
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
94726
Choy
Survey, purification, and prop ...
Escherichia acidilactici, Escherichia coli, Klebsiella aerogenes, Neisseria meningitidis, Saccharomyces cerevisiae
Can. J. Biochem. Cell Biol.
61
1292-1303
1983
-
-
-
-
-
-
-
-
-
-
7
-
-
31
-
-
5
-
-
-
7
-
35
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
5
-
-
7
-
35
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94724
Lau
Effects of fluoride on sugar p ...
Escherichia coli, Neisseria meningitidis
Int. J. Biochem.
14
565-567
1982
-
-
-
-
-
-
2
-
1
-
-
-
-
5
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94723
Lee
-
Molecular weight and some phys ...
Escherichia coli, Neisseria meningitidis
J. Biol. Chem.
250
3729-3737
1975
-
-
-
1
-
-
11
16
-
-
4
-
-
2
-
-
1
-
-
-
1
1
32
1
-
-
4
-
1
1
3
-
-
-
-
-
-
-
-
1
-
-
-
11
-
16
-
-
4
-
-
-
-
1
-
-
1
1
32
1
-
-
4
-
1
1
3
-
-
-
-
-
-
-
94722
Lee
Sugar phosphate phosphohydrola ...
Neisseria meningitidis
J. Biol. Chem.
242
2264-2271
1967
-
-
-
-
-
-
-
-
3
-
-
-
-
3
-
-
1
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
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