Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | activates DNase1/3-like activity | Mus musculus | |
heparin | activates DNase1 | Mus musculus |
Cloned (Comment) | Organism |
---|---|
NIH-3T3 cells with expression vectors for murine DNase1 and DNase1/3 cDNA | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | DNase1 knockout mice, contain residual nuclease activity. Addition of aprotinin or plasminogen activator inhibitor 1 to the sera of DNase1 KO mice completes chromatolysis to mononucleosomes and even to oligonucleotides | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | inhibits DNase1 as a result of plasmin inhibition | Mus musculus | |
heparin | directly inhibits recombinant DNase1/3 | Homo sapiens | |
heparin | directly inhibits recombinant DNase1/3 | Mus musculus | |
additional information | DNase1/3-like nuclease is inhibited by proteolysis of DNA-bound structural proteins but not by thrombin. When serum frozen at -20°C to thawing to room temperature and subsequently stored at 4°C, it looses its DNase1/3-like activity within 2 weeks | Mus musculus | |
NaCl | increasing concentrations of NaCl (approximately half activity in the presence of 50 mM NaCl) have a greater inhibitory influence on rmDNase1/3 than on rmDNase1 (approximately half activity in the presence of 150 mM NaCl) | Mus musculus | |
plasmin | directly inhibits recombinant DNase1/3, but does not inactivate recombinant DNase1 | Mus musculus | |
Tris | increasing concentrations of Tris (approximately half activity in the presence of 80 mM Tris) have a greater inhibitory influence on rmDNase1/3 than on rmDNase1 (no inhibitory influence of Tris) | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus | |
Mn2+ | activity of recombinant DNase1 in the presence of Mn2+ is increased only slightly, whereas recombinant DNase1/3 displays strongly enhanced nucleolysis | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29800 | - |
calculated molecular mass of DNase1 | Mus musculus |
33100 | - |
calculated molecular mass of DNase1/3 | Mus musculus |
34000 | - |
DNase1/3, by DPZ | Mus musculus |
37000 | - |
DNase1, by DPZ | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | DNase1 and DNase1/3 may substitute or cooperate with each other during DNA degradation, providing effective clearance after exposure or release from dying cells | ? | - |
? | |
additional information | Mus musculus C57BL/6 | DNase1 and DNase1/3 may substitute or cooperate with each other during DNA degradation, providing effective clearance after exposure or release from dying cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | O55070 | C57BL/6 mice | - |
Mus musculus C57BL/6 | O55070 | C57BL/6 mice | - |
Purification (Comment) | Organism |
---|---|
DNase1/3-like nuclease, by affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | high DNase1 content | Mus musculus | - |
serum | contains two nucleases, DNase1 and DNase1/3. DNase1/3 is not N-glycosylated, whereas DNase1 is di-N-glycosylated | Mus musculus | - |
serum | DNase1/3 | Homo sapiens | - |
spleen | high DNase1/3 content | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chromatin + H2O | recombinant DNase1 degrades chromatin effectively only in cooperation with serine proteases, such as plasmin or thrombin, which remove DNA-bound proteins. Recombinant DNase1/3 degrades chromatin without proteolytic help | Mus musculus | ? | - |
? | |
chromatin + H2O | recombinant DNase1 degrades chromatin effectively only in cooperation with serine proteases, such as plasmin or thrombin, which remove DNA-bound proteins. Recombinant DNase1/3 degrades chromatin without proteolytic help | Mus musculus C57BL/6 | ? | - |
? | |
additional information | DNase1 and DNase1/3 may substitute or cooperate with each other during DNA degradation, providing effective clearance after exposure or release from dying cells | Mus musculus | ? | - |
? | |
additional information | DNase1 and DNase1/3 may substitute or cooperate with each other during DNA degradation, providing effective clearance after exposure or release from dying cells | Mus musculus C57BL/6 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dnase1 | - |
Homo sapiens |
Dnase1 | - |
Mus musculus |
DNase1-like 3 | - |
Homo sapiens |
DNase1-like 3 | - |
Mus musculus |
DNase1/3 | - |
Homo sapiens |
DNase1/3 | - |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | pH optimum of recombinant DNase1 is 6.5-7.5 in the presence of Mg2+, and 7.5-8.5 in the presence of Mn2+. PH optimum of recombinant DNase1/3 in the presence of Mg2+ shifts from pH 4.5-5.5 to pH 5.5-6.5 by Mn2+ | Mus musculus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mus musculus | DNase1 | - |
4.9 |
Mus musculus | DNase1/3 | - |
8.7 |