Protein Variants | Comment | Organism |
---|---|---|
L109P | mutation shifts the substrate-preference from medium-to-long acyl chains to shorter acyl-chains of triglyceride and p-nitrophenyl ester, and increases the preference for aromatic-amino acid-derived esters. kcat for lauroyl-CoA is 17.8fold lower than wild-type value, Km-value for lauroyl-CoA is 1.3fold higher than wild-type value | Escherichia coli |
P110A | kcat for lauroyl-CoA is 2.7fold lower than wild-type value, Km-value for lauroyl-CoA is 1.3fold lower than wild-type value | Escherichia coli |
P110A/L109P | kcat for lauroyl-CoA is 12fold lower than wild-type value, Km-value for lauroyl-CoA is 2fold lower than wild-type value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
lauroyl-CoA | mutant enzyme P110A/L109P | Escherichia coli | |
0.113 | - |
lauroyl-CoA | mutant enzyme P110A | Escherichia coli | |
0.146 | - |
lauroyl-CoA | native enzyme | Escherichia coli | |
0.185 | - |
lauroyl-CoA | mutant enzyme L109P | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ADA1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lauroyl-CoA + H2O | - |
Escherichia coli | CoA + laurate | - |
? | |
stearoyl-CoA + H2O | - |
Escherichia coli | CoA + stearate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thioesterase I/protease I/lysophospholipase L1 | multifunctional enzyme with thioesterase, esterase, arylesterase, protease and lysophospholipase activities | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | 2.1 | lauroyl-CoA | native enzyme | Escherichia coli | |
0.57 | - |
lauroyl-CoA | mutant enzyme L109P | Escherichia coli | |
0.84 | - |
lauroyl-CoA | mutant enzyme P110A/L109P | Escherichia coli | |
3.8 | - |
lauroyl-CoA | mutant enzyme P110A | Escherichia coli | |
10.13 | - |
lauroyl-CoA | native enzyme | Escherichia coli |