BRENDA - Enzyme Database
show all sequences of 3.1.2.10

Formate metabolism. I. Formyl coenzyme A an intermediate in the formate-dependent decomposition of acetyl phosphate in Clostridium kluyveri

Sly, W.S.; Stadtman, E.R.; J. Biol. Chem. 238, 2632-2638 (1963)

Data extracted from this reference:

General Stability
General Stability
Organism
dilution to 0.1 mg/ml, 50% loss of activity in 2 h
Clostridium kluyveri
passage over Sephadex or dialysis for longer than 4 h, loss of activity up to 50%
Clostridium kluyveri
reducing agents, crystalline bovine serum albumin have no stabilizing effect
Clostridium kluyveri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
formyl-CoA
-
Clostridium kluyveri
0.08
-
Formylpantetheine
-
Clostridium kluyveri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formyl-CoA + H2O
Clostridium kluyveri
formate-dependent decomposition of acetyl phosphate
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium aminobutyricum
-
-
-
Clostridium kluyveri
-
-
-
no activity in Clostridium cylindrosporum
-
-
-
no activity in Clostridium sticklandii
-
-
-
no activity in Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Clostridium kluyveri
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
90
100
-
Clostridium kluyveri
Storage Stability
Storage Stability
Organism
-20°C, stable for 2 months, in liquid nitrogen, pH 6.5, up to 6 months
Clostridium kluyveri
0°C, concentrated solutions, 10 mg/ml, more than 1 week, partially purified
Clostridium kluyveri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formyl-CoA + H2O
-
80970
Clostridium kluyveri
CoA + formate
-
80970
Clostridium kluyveri
?
formyl-CoA + H2O
formate-dependent decomposition of acetyl phosphate
80970
Clostridium kluyveri
?
-
-
-
-
formylpantetheine + H2O
-
80970
Clostridium kluyveri
pantetheine + formate
-
80970
Clostridium kluyveri
?
S-formylglutathione + H2O
3% of the rate observed with formylpantetheine
80970
Clostridium kluyveri
glutathione + formate
-
80970
Clostridium kluyveri
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
5 min, 80% loss of activity
Clostridium kluyveri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.1
-
Clostridium kluyveri
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7.5
38% and 27% of optimal activity at pH 6.0 and pH 7.5
Clostridium kluyveri
General Stability (protein specific)
General Stability
Organism
dilution to 0.1 mg/ml, 50% loss of activity in 2 h
Clostridium kluyveri
passage over Sephadex or dialysis for longer than 4 h, loss of activity up to 50%
Clostridium kluyveri
reducing agents, crystalline bovine serum albumin have no stabilizing effect
Clostridium kluyveri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
formyl-CoA
-
Clostridium kluyveri
0.08
-
Formylpantetheine
-
Clostridium kluyveri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formyl-CoA + H2O
Clostridium kluyveri
formate-dependent decomposition of acetyl phosphate
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Clostridium kluyveri
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
90
100
-
Clostridium kluyveri
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for 2 months, in liquid nitrogen, pH 6.5, up to 6 months
Clostridium kluyveri
0°C, concentrated solutions, 10 mg/ml, more than 1 week, partially purified
Clostridium kluyveri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formyl-CoA + H2O
-
80970
Clostridium kluyveri
CoA + formate
-
80970
Clostridium kluyveri
?
formyl-CoA + H2O
formate-dependent decomposition of acetyl phosphate
80970
Clostridium kluyveri
?
-
-
-
-
formylpantetheine + H2O
-
80970
Clostridium kluyveri
pantetheine + formate
-
80970
Clostridium kluyveri
?
S-formylglutathione + H2O
3% of the rate observed with formylpantetheine
80970
Clostridium kluyveri
glutathione + formate
-
80970
Clostridium kluyveri
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
5 min, 80% loss of activity
Clostridium kluyveri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.1
-
Clostridium kluyveri
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7.5
38% and 27% of optimal activity at pH 6.0 and pH 7.5
Clostridium kluyveri
Other publictions for EC 3.1.2.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
80970
Sly
Formate metabolism. I. Formyl ...
Clostridium aminobutyricum, Clostridium kluyveri, no activity in Clostridium cylindrosporum, no activity in Clostridium sticklandii, no activity in Escherichia coli
J. Biol. Chem.
238
2632-2638
1963
-
-
-
-
-
3
-
2
-
-
-
1
-
5
-
-
1
-
-
-
1
2
4
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
2
-
-
-
1
-
-
-
1
-
-
1
2
4
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-