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Literature summary for 3.1.2.10 extracted from
- Formate metabolism. I. Formyl coenzyme A an intermediate in the formate-dependent decomposition of acetyl phosphate in Clostridium kluyveri (1963), J. Biol. Chem., 238, 2632-2638.
General Stability
| General Stability | Organism |
|---|---|
| dilution to 0.1 mg/ml, 50% loss of activity in 2 h | Clostridium kluyveri |
| passage over Sephadex or dialysis for longer than 4 h, loss of activity up to 50% | Clostridium kluyveri |
| reducing agents, crystalline bovine serum albumin have no stabilizing effect | Clostridium kluyveri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
formyl-CoA | - |
Clostridium kluyveri |  |
| 0.08 | - |
Formylpantetheine | - |
Clostridium kluyveri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formyl-CoA + H2O | Clostridium kluyveri | formate-dependent decomposition of acetyl phosphate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium aminobutyricum | - |
- |
- |
| Clostridium kluyveri | - |
- |
- |
| no activity in Clostridium cylindrosporum | - |
- |
- |
| no activity in Clostridium sticklandii | - |
- |
- |
| no activity in Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Clostridium kluyveri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 90 | 100 | - |
Clostridium kluyveri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 2 months, in liquid nitrogen, pH 6.5, up to 6 months | Clostridium kluyveri |
| 0°C, concentrated solutions, 10 mg/ml, more than 1 week, partially purified | Clostridium kluyveri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formyl-CoA + H2O | - |
Clostridium kluyveri | CoA + formate | - |
? | |
| formyl-CoA + H2O | formate-dependent decomposition of acetyl phosphate | Clostridium kluyveri | ? | - |
? | |
| formylpantetheine + H2O | - |
Clostridium kluyveri | pantetheine + formate | - |
? | |
| S-formylglutathione + H2O | 3% of the rate observed with formylpantetheine | Clostridium kluyveri | glutathione + formate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min, 80% loss of activity | Clostridium kluyveri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.1 | - |
Clostridium kluyveri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | 38% and 27% of optimal activity at pH 6.0 and pH 7.5 | Clostridium kluyveri |
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Release 2023.1 (January 2023)
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