Information on EC 3.1.2.10 - formyl-CoA hydrolase

for references in articles please use BRENDA:EC3.1.2.10
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The expected taxonomic range for this enzyme is: Clostridium

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EC NUMBER
COMMENTARY hide
3.1.2.10
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RECOMMENDED NAME
GeneOntology No.
formyl-CoA hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
formyl-CoA + H2O = CoA + formate
show the reaction diagram
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-
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of thioester
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
fatty acid alpha-oxidation II
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fatty acid alpha-oxidation III
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oxalate degradation III
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oxalate degradation VI
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Glyoxylate and dicarboxylate metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
formyl-CoA hydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9025-91-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Clostridium cylindrosporum
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Manually annotated by BRENDA team
no activity in Clostridium sticklandii
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Manually annotated by BRENDA team
no activity in Escherichia coli
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
formyl-CoA + H2O
?
show the reaction diagram
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formate-dependent decomposition of acetyl phosphate
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formyl-CoA + H2O
CoA + formate
show the reaction diagram
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-
-
?
formylpantetheine + H2O
pantetheine + formate
show the reaction diagram
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-
-
?
S-formylglutathione + H2O
glutathione + formate
show the reaction diagram
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3% of the rate observed with formylpantetheine
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?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
formyl-CoA + H2O
?
show the reaction diagram
-
formate-dependent decomposition of acetyl phosphate
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-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045
formyl-CoA
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0.08
Formylpantetheine
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 7.1
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-
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
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38% and 27% of optimal activity at pH 6.0 and pH 7.5
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
5 min, 80% loss of activity
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dilution to 0.1 mg/ml, 50% loss of activity in 2 h
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passage over Sephadex or dialysis for longer than 4 h, loss of activity up to 50%
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reducing agents, crystalline bovine serum albumin have no stabilizing effect
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for 2 months, in liquid nitrogen, pH 6.5, up to 6 months
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0°C, concentrated solutions, 10 mg/ml, more than 1 week, partially purified
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 3.1.2.10)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)