Information on EC 3.1.2.10 - formyl-CoA hydrolase

for references in articles please use BRENDA:EC3.1.2.10
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Clostridium

EC NUMBER
COMMENTARY hide
3.1.2.10
-
RECOMMENDED NAME
GeneOntology No.
formyl-CoA hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
formyl-CoA + H2O = CoA + formate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of thioester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
fatty acid alpha-oxidation II
-
-
fatty acid alpha-oxidation III
-
-
oxalate degradation III
-
-
Glyoxylate and dicarboxylate metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
formyl-CoA hydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-91-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Clostridium cylindrosporum
-
-
-
Manually annotated by BRENDA team
no activity in Clostridium sticklandii
-
-
-
Manually annotated by BRENDA team
no activity in Escherichia coli
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
formyl-CoA + H2O
?
show the reaction diagram
-
formate-dependent decomposition of acetyl phosphate
-
-
-
formyl-CoA + H2O
CoA + formate
show the reaction diagram
-
-
-
?
formylpantetheine + H2O
pantetheine + formate
show the reaction diagram
-
-
-
?
S-formylglutathione + H2O
glutathione + formate
show the reaction diagram
-
3% of the rate observed with formylpantetheine
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
formyl-CoA + H2O
?
show the reaction diagram
-
formate-dependent decomposition of acetyl phosphate
-
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045
formyl-CoA
-
-
0.08
Formylpantetheine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 7.1
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
-
38% and 27% of optimal activity at pH 6.0 and pH 7.5
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
5 min, 80% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dilution to 0.1 mg/ml, 50% loss of activity in 2 h
-
passage over Sephadex or dialysis for longer than 4 h, loss of activity up to 50%
-
reducing agents, crystalline bovine serum albumin have no stabilizing effect
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for 2 months, in liquid nitrogen, pH 6.5, up to 6 months
-
0°C, concentrated solutions, 10 mg/ml, more than 1 week, partially purified
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE