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Information on EC 3.1.2.10 - formyl-CoA hydrolase for references in articles please use BRENDA:EC3.1.2.10
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The expected taxonomic range for this enzyme is: Clostridium
Synonyms
formyl coenzyme A hydrolase, hydrolase, formyl coenzyme A,
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formyl coenzyme A hydrolase
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hydrolase, formyl coenzyme A
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formyl-CoA + H2O = CoA + formate
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hydrolysis of thioester
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formyl-CoA + H2O
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formate-dependent decomposition of acetyl phosphate
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formyl-CoA + H2O
CoA + formate
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formylpantetheine + H2O
pantetheine + formate
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S-formylglutathione + H2O
glutathione + formate
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3% of the rate observed with formylpantetheine
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formyl-CoA + H2O
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formate-dependent decomposition of acetyl phosphate
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0.08
Formylpantetheine
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6 - 7.5
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38% and 27% of optimal activity at pH 6.0 and pH 7.5
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no activity in Clostridium cylindrosporum
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no activity in Clostridium sticklandii
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no activity in Escherichia coli
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55
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5 min, 80% loss of activity
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dilution to 0.1 mg/ml, 50% loss of activity in 2 h
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passage over Sephadex or dialysis for longer than 4 h, loss of activity up to 50%
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reducing agents, crystalline bovine serum albumin have no stabilizing effect
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-20°C, stable for 2 months, in liquid nitrogen, pH 6.5, up to 6 months
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0°C, concentrated solutions, 10 mg/ml, more than 1 week, partially purified
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Sly, W.S.; Stadtman, E.R.
Formate metabolism. I. Formyl coenzyme A an intermediate in the formate-dependent decomposition of acetyl phosphate in Clostridium kluyveri
J. Biol. Chem.
238
2632-2638
1963
Clostridium aminobutyricum, Clostridium kluyveri, no activity in Clostridium cylindrosporum, no activity in Clostridium sticklandii, no activity in Escherichia coli
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3.1.2.10 formyl-CoA hydrolase
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