Cloned (Comment) | Organism |
---|---|
overexpressed in BL21(DE3) cells | uncultured bacterium |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.06 | - |
p-nitrophenyl butyrate | Vmax value is 493.6 microM/min | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | Q0GMU2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
uncultured bacterium |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl acetate + H2O | using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) | uncultured bacterium | ? | - |
? | |
p-nitrophenyl butyrate + H2O | using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) | uncultured bacterium | ? | - |
? | |
p-nitrophenyl caproate + H2O | using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) | uncultured bacterium | ? | - |
? | |
p-nitrophenyl caprylate + H2O | using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) | uncultured bacterium | ? | - |
? | |
p-nitrophenyl decanoate + H2O | using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) | uncultured bacterium | ? | - |
? | |
p-nitrophenyl palmitate + H2O | using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) | uncultured bacterium | ? | - |
? | |
p-nitrophenyl stearate + H2O | using various p-nitrophenyl esters as substrates. Enzyme efficiently hydrolyzes p-nitrophenyl acetate (75%), p-nitrophenyl butyrate (100%), p-nitrophenyl carproate (55%) and p-nitrophenyl caprylate (50%), a lower level of hydrolysis is observed for p-nitrophenyl decanoate (25%), p-nitrophenyl palmitate (2%) and p-nitrophenyl stearate (2%) | uncultured bacterium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EstE5 | - |
uncultured bacterium |
EstE5 protein | - |
uncultured bacterium |
hormone-sensitive lipase | - |
uncultured bacterium |
HSL | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
uncultured bacterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | purified enzyme is broadly stable within a pH range of 5.5-9.0 | uncultured bacterium |