Crystallization (Comment) | Organism |
---|---|
purified catalytic core of the enzyme at 10 mg/ml, hanging drop vapour diffusion method, room temperature, protein solution: sodium acetate, pH 5.0, reservoir solution: potassium/sodium tartrate 1.1 M, TES buffer 0.1 M, pH 8.2, in the ratio 2:1:1 with a Trixton X-100 solution, few weeks, X-ray diffraction structure determination and analysis at 1.9 A, structure model | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | Q99034 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetylxylan + 3 H2O = 3 acetic acid + xylan | catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin, reaction mechanism, enzyme is a serine esterase containing an oxyanion hole and a catalytic triad build of Ser90, His187, and Asp175, substrate binding model | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated xylan + H2O | catalytic mechanism, substrate with mono- and double acetylated residues, the enzyme cannot remove acetyl groups located close to large side groups such as 4-O-methylglucuronic acid | Trichoderma reesei | xylan + acetate | - |
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