Information on EC 3.1.1.72 - acetylxylan esterase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.1.72
-
RECOMMENDED NAME
GeneOntology No.
acetylxylan esterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Deacetylation of xylans and xylo-oligosaccharides
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
SYSTEMATIC NAME
IUBMB Comments
acetylxylan esterase
Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-napthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.
CAS REGISTRY NUMBER
COMMENTARY hide
188959-24-2
-
9000-82-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain ATCC 20236
-
-
Manually annotated by BRENDA team
strain ATCC 24919
-
-
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
PS213
-
-
Manually annotated by BRENDA team
gene Cah
-
-
Manually annotated by BRENDA team
subsp. tengcongensis, gene TTE0866
UniProt
Manually annotated by BRENDA team
subsp. tengcongensis, gene TTE0866
UniProt
Manually annotated by BRENDA team
gene xynA, enzyme with a double specificity as xylanase and acetylxylan esterase
SwissProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
V583
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene xynS20E
UniProt
Manually annotated by BRENDA team
no activity in Butyrivibrio fibrisolvens
-
-
-
Manually annotated by BRENDA team
no activity in Butyrivibrio fibrisolvens NCFB 2249
-
-
-
Manually annotated by BRENDA team
no activity in Fibrobacter succinogenes
-
-
-
Manually annotated by BRENDA team
no activity in Fibrobacter succinogenes S85
-
-
-
Manually annotated by BRENDA team
no activity in Neocallimastix sp.
-
-
-
Manually annotated by BRENDA team
no activity in Neocallimastix sp. MC-2
-
-
-
Manually annotated by BRENDA team
no activity in Orpinomyces sp.
-
-
-
Manually annotated by BRENDA team
no activity in Orpinomyces sp. PC-3
-
-
-
Manually annotated by BRENDA team
gene axe1
UniProt
Manually annotated by BRENDA team
very low activity
-
-
Manually annotated by BRENDA team
strain ATTC 21713, strain NRCC2258
-
-
Manually annotated by BRENDA team
strain C-248, strain C-254
-
-
Manually annotated by BRENDA team
very low activity
-
-
Manually annotated by BRENDA team
gene axeA
-
-
Manually annotated by BRENDA team
3 isozymes
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(glucose)4 + triacetin
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(mannose)2 + triacetin
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(mannose)5 + triacetin
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(mannose)6 + triacetin
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(xylose)2 + triacetin
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
(xylose)5 + triacetin
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
show the reaction diagram
1-naphthyl acetate + H2O
naphthol + acetate
show the reaction diagram
-
-
-
?
1-naphthyl caprate + H2O
naphthol + caprate
show the reaction diagram
-
-
-
?
1-naphthyl myristate + H2O
naphthol + myristate
show the reaction diagram
-
-
-
?
2,3,4-tri-O-acetyl beta-D-xylopyranoside + H2O
4-O-acetyl beta-D-xylopyranoside + acetate
show the reaction diagram
-
regioselective double deacetylation, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
2,3,4-tri-O-acetyl-beta-D-xylopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-di-O-acetyl beta-D-xylopyranoside + H2O
4-O-acetyl beta-D-xylopyranoside + acetate
show the reaction diagram
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
2,4-di-O-acetyl-methyl beta-D-xylopyranoside + H2O
?
show the reaction diagram
-
-
-
?
2-deoxy-2-fluoro-3,4-di-O-acetyl beta-D-xylopyranoside + H2O
2-deoxy-2-fluoro-4-O-acetyl beta-D-xylopyranoside + acetate
show the reaction diagram
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
2-deoxy-3,4-di-O-acetyl beta-D-xylopyranoside + H2O
2-deoxy-4-O-acetyl beta-D-xylopyranoside + 2-deoxy-3-O-acetyl beta-D-xylopyranoside + acetate
show the reaction diagram
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
acetylated xylopyranosides in ratio 2:1
?
2-naphthyl acetate + H2O
2-naphthol + acetate
show the reaction diagram
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
acetate + 4-nitrophenyl-beta-D-xylopyranoside
show the reaction diagram
3,4-di-O-acetyl beta-D-xylopyranoside + H2O
4-O-acetyl beta-D-xylopyranoside + acetate
show the reaction diagram
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
3,4-di-O-acetyl-methyl beta-D-xylopyranoside + H2O
?
show the reaction diagram
-
-
-
?
3-deoxy-3-fluoro-2,4-di-O-acetyl beta-D-xylopyranoside + H2O
3-deoxy-3-fluoro-4-O-acetyl beta-D-xylopyranoside + acetate
show the reaction diagram
-
regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity
-
?
3-naphthyl acetate + H2O
3-naphthol + acetate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl acetate
acetate + 4-methylumbelliferol
show the reaction diagram
4-methylumbelliferyl acetate + H2O
4-methylumbelliferol + acetate
show the reaction diagram
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
show the reaction diagram
4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
show the reaction diagram
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
show the reaction diagram
4-nitrophenyl 3-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
show the reaction diagram
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
show the reaction diagram
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl beta-D-xylose + acetate
show the reaction diagram
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl alpha-L-arabinose + acetate
show the reaction diagram
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
show the reaction diagram
4-nitrophenyl glucopyranoside + H2O
4-nitrophenol + glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
show the reaction diagram
-
-
-
?
4-nitrophenyl xylopyranoside + H2O
4-nitrophenol + xylose
show the reaction diagram
-
-
-
-
?
4-O-methyl-D-glucuronosyl-alpha-1,2-D-xylopyranosyl-beta-1,4-xylopyranosyl-beta-1,4-xylopyranose + H2O
-
show the reaction diagram
-
-
-
-
?
7-aminocephalosporanic acid
acetate + ?
show the reaction diagram
-
-
-
-
-
7-aminocephalosporanic acid + H2O
?
show the reaction diagram
Ac-MeGlcAXylan + H2O
?
show the reaction diagram
acetyl-xylobiose
acetate + ?
show the reaction diagram
-
very low activity
-
-
-
acetylated birch-wood xylan + H2O
acetate + birch-wood xylan
show the reaction diagram
acetylated birchwood xylan + H2O
acetate + birchwood xylan
show the reaction diagram
-
-
-
?
acetylated chitin + H2O
acetate + chitin
show the reaction diagram
enzymatic deacetylation of chitinous substrates by AxeA is influenced by both substrate accessibility and mode of enzyme action. Barely acts on solid pure chitin whatever its form: native alpha-chitin and whiskers or native beta-chitin. But when chemically treated in strong alkali, chitin is partially deacetylated. The longer the alkali treatment, the more efficient the deacetylation of alkali-treated chitins
-
-
?
acetylated chitosan + H2O
? + acetate
show the reaction diagram
acetylated colloidal chitin + H2O
? + acetate
show the reaction diagram
acetylated glycol chitin + H2O
? + acetate
show the reaction diagram
acetylated xylan + H2O
?
show the reaction diagram
acetylated xylan + H2O
partially deacetylated xylan + acetate
show the reaction diagram
acetylated xylan + H2O
xylan + acetate
show the reaction diagram
acetylated xylooligosaccharide + H2O
?
show the reaction diagram
acetylated xylooligosaccharides + H2O
?
show the reaction diagram
acetylxylan
acetate + ?
show the reaction diagram
acetylxylan + H2O
?
show the reaction diagram
acetylxylanacetate + ?
?
show the reaction diagram
alpha-naphthyl acetate
acetate + 2-naphthol
show the reaction diagram
alpha-naphthyl acetate + H2O
naphthol + acetate
show the reaction diagram
alpha-naphthyl propionate + H2O
naphthol + propionate
show the reaction diagram
amyl acetate
acetate + amyl alcohol
show the reaction diagram
-
-
-
-
-
aspen acetyl glucuronoxylan + H2O
?
show the reaction diagram
beechwood acetyl glucuronoxylan + H2O
?
show the reaction diagram
beechwood xylan + H2O
?
show the reaction diagram
beta-D-xylose tetraacetate + H2O
?
show the reaction diagram
beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc + H2O
?
show the reaction diagram
-
native AxeA and two variants display about 50% deacetylation of the oligohexamer substrate after reaction at 50C for 24 h in diluted culture supernatant. Characterization of the deacetylation products from reaction of wild-type and mutant AXEs, overview
-
-
?
beta-naphthyl propionate + H2O
naphthol + propionate
show the reaction diagram
birch acetyl glucuronoxylan + H2O
?
show the reaction diagram
-
positional specificity of the enzyme
-
-
?
birchwood acetyl glucuronoxylan + H2O
?
show the reaction diagram
birchwood xylan + H2O
?
show the reaction diagram
-
-
-
?
butyl acetate
acetate + butanol
show the reaction diagram
-
-
-
-
-
cellobiose octaacetate + H2O
?
show the reaction diagram
-
-
-
?
cellulose acetate
acetate + ?
show the reaction diagram
-
-
-
-
-
cellulose acetate + H2O
?
show the reaction diagram
different samples, CA0.88 is the best substrate, efficient complete degradation when the enzyme is mixed with a cellulolytic endo-1,4-beta-glucanase, EC 3.2.1.4
-
-
?
cellulose acetate + H2O
cellulose + acetate
show the reaction diagram
cephalosporin C
acetate + ?
show the reaction diagram
-
-
-
-
-
cephalosporin C + H2O
deacetylcephalosporin C + acetate
show the reaction diagram
D-galactose pentaacetate + H2O
D-galactose + acetate
show the reaction diagram
low activity
-
?
D-glucose penta-acetate + H2O
?
show the reaction diagram
-
deacetylation
-
-
?
D-glucose pentaacetate + H2O
?
show the reaction diagram
D-glucose pentaacetate + H2O
D-glucose + acetate
show the reaction diagram
-
-
-
?
D-xylose tetraacetate + H2O
?
show the reaction diagram
glucose pentaacetate
acetate + ?
show the reaction diagram
glucose pentaacetate + H2O
?
show the reaction diagram
-
-
-
?
glucose pentaacetate + H2O
glucose + acetate
show the reaction diagram
-
-
?
glucose pentacetate + H2O
?
show the reaction diagram
-
-
-
-
?
hardwood acetyl glucuronoxylan
?
show the reaction diagram
insoluble oat spelt xylan
?
show the reaction diagram
-
-
-
?
methyl 2,3,4-tri-O-acetyl-beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
show the reaction diagram
methyl 2,3-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 2-O-acetyl-beta-D-xylopyranoside + methyl 3-O-acetyl-beta-D-xylopyranoside
show the reaction diagram
methyl 2,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
show the reaction diagram
methyl 3,4-di-O-acetyl beta-D-xylopyranoside
acetate + methyl 4-O-acetyl-beta-D-xylopyranoside
show the reaction diagram
methyl beta-cellobiose + triacetin
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
methyl beta-cellobiose + vinyl acetate
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 2,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 2-deoxy-2-fluoro-3,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 2-deoxy-3,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 3 deoxy-3-fluoro-2,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 3,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 3-deoxy-2,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-xylopyranoside + triacetin
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
methyl beta-xylopyranoside + vinyl acetate
?
show the reaction diagram
-
transesterification in a microemulsion of n-hexane-vinyl acetate-sodium dioctylsulfosuccinate-water, water content of 0.4% v/v is absolutely required for activity
-
?
N,N'-diacetylchitobiose + H2O
?
show the reaction diagram
-
-
-
-
?
N,N'-diacetylchitobiose + H2O
chitobiose + acetate
show the reaction diagram
-
-
?
oat spelt xylan + H2O
?
show the reaction diagram
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
p-nitrophenyl acetate + H2O
p-nitrophenyl + acetate
show the reaction diagram
p-nitrophenyl butyrate + H2O
p-nitrophenol + butyrate
show the reaction diagram
p-nitrophenyl caprylate + H2O
p-nitrophenol + caprylate
show the reaction diagram
p-nitrophenyl palmitate + H2O
p-nitrophenol + palmitate
show the reaction diagram
penta-O-acetyl-D-glucose
acetate + ?
show the reaction diagram
-
-
-
-
-
sucrose octaacetate + H2O
?
show the reaction diagram
-
-
-
?
tetraacetyl-xylopyranoside + H2O
? + acetate
show the reaction diagram
-
-
-
?
tri-O-acetyl-D-galactal + H2O
D-galactal + acetate
show the reaction diagram
-
-
?
tri-O-acetylglycerol
acetate + ?
show the reaction diagram
-
-
-
-
-
triacetin
acetate + ?
show the reaction diagram
-
-
-
-
-
xanthan + H2O
?
show the reaction diagram
xylan + H2O
?
show the reaction diagram
-
deacetylation of acetylated oat spelt xylan and DMSO-extracted beechwood xylan
-
-
?
xylose tetraacetate
acetate + ?
show the reaction diagram
xylose tetraacetate + H2O
?
show the reaction diagram
xylose tetraacetate + H2O
xylose + acetate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetylated xylan + H2O
partially deacetylated xylan + acetate
show the reaction diagram
acetylated xylan + H2O
xylan + acetate
show the reaction diagram
Q8GH59
enzyme is a key component of xylan and cell wall degradation
-
?
methyl beta-D-xylopyranoside 2,3,4-tri-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 2,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 2-deoxy-2-fluoro-3,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 2-deoxy-3,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 3 deoxy-3-fluoro-2,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 3,4-di-O-acetate + H2O
?
show the reaction diagram
methyl beta-D-xylopyranoside 3-deoxy-2,4-di-O-acetate + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
9.6% activation at 1 mM
Fe2+
-
about 25% activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ba2+
-
low inhibition at 1 mM
Cd2+
-
low inhibition at 1 mM
CO2
50% inhibition at 1 mM
di-isopropylfluorophosphate
-
diethyl phosphate
AXE shows low sensitivity to the organophosphate, binding structure, overview
diethyldicarbonate
-
-
diisopropyl fluorophosphate
-
-
dimethyl 4-nitrophenyl phosphate
-
Fe3+
-
strong inhibition at 1 mM
glucose
represses enzyme expression, gene axeII
guanidinium hydrochloride
-
3.5-4.0 M, inactivation
methyl beta-D-xylopyranoside
-
-
NiCl2
-
1 mM, 8% inhibition
Paraoxon
binding structure, overview
Pb(NO3)2
-
1 mM
Pepsin
-
25-36% inhibition by 0.001 mg/0.05 ml pepsin
-
phenylmethanesulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
Triton X-100
Trypsin
-
Urea
-
7.0 M, inactivation
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
109% of initial activity
corncob
-
in the medium, induces enzyme production
-
Mg2+
112% of initial activity
papain
-
enzyme activity is increased by 17-29% by 0.001 mg/0.05 ml papain
-
xylan
xylitol
slightly induces enzyme expression, gene axeII
xylose
slightly induces enzyme expression, gene axeII
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7
2-naphthyl acetate
1
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
-
0.092 - 11
4-nitrophenyl acetate
0.11
4-nitrophenyl butyrate
pH 8.0, 40C, recombinant enzyme
0.3077 - 0.56
4-yethylumbelliferyl acetate
0.056 - 2.66
Alpha-naphthyl acetate
16.72
birchwood xylan
pH 8.2, 58C
-
0.00186
p-nitrophenyl acetate
-
30C, pH 8.0
0.07 - 7
tetraacetyl-xylopyranoside
additional information
additional information
1.42 mg/mL with glycol chitin as substrate, in 50 mM Tris-HCl buffer, pH 7.0, at 60C for 20 min
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
118
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
-