Cloned (Comment) | Organism |
---|---|
overexpression in Streptomyces lividans strain IAF43, secretion to the culture medium | Streptomyces lividans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methyl beta-D-xylopyranoside | - |
Streptomyces lividans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces lividans | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetylxylan + 3 H2O = 3 acetic acid + xylan | catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. | Streptomyces lividans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000045 | - |
purified enzyme, substrate 2,3,4-tri-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
0.000056 | - |
purified enzyme, substrate 2-deoxy-3,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
0.000063 | - |
purified enzyme, substrate 3-deoxy-2,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
0.00016 | - |
purified enzyme, substrate 2-deoxy-2-fluoro-3,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
0.0016 | - |
purified enzyme, substrate 3-deoxy-3-fluoro-2,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
0.034 | - |
purified enzyme, substrate 3,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
0.175 | - |
purified enzyme, substrate 2,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,4-tri-O-acetyl beta-D-xylopyranoside + H2O | regioselective double deacetylation, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 4-O-acetyl beta-D-xylopyranoside + acetate | - |
? | |
2,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 4-O-acetyl beta-D-xylopyranoside + acetate | - |
? | |
2-deoxy-2-fluoro-3,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 2-deoxy-2-fluoro-4-O-acetyl beta-D-xylopyranoside + acetate | - |
? | |
2-deoxy-3,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 2-deoxy-4-O-acetyl beta-D-xylopyranoside + 2-deoxy-3-O-acetyl beta-D-xylopyranoside + acetate | acetylated xylopyranosides in ratio 2:1 | ? | |
3,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 4-O-acetyl beta-D-xylopyranoside + acetate | - |
? | |
3-deoxy-3-fluoro-2,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 3-deoxy-3-fluoro-4-O-acetyl beta-D-xylopyranoside + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AcXE | - |
Streptomyces lividans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Streptomyces lividans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Streptomyces lividans |