General Stability | Organism |
---|---|
lyophilization and reconstitution results in loss of 30% of activity | Rattus norvegicus |
single cycle of freeze-thaw results in little loss of activity, repeated freeze-thaw results in gradual loss of activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Butanedione | irreversibly inactivated | Rattus norvegicus | |
9-acetamido-N-acetyl-O-acetylneuraminate | no effect up to 5 mM | Rattus norvegicus | |
bis-p-nitrophenyl phosphate | little effect on the activity | Rattus norvegicus | |
CHAPS | lowers the activity by 40% | Rattus norvegicus | |
deoxycholate | 75-80% loss of activity at 2% concentration | Rattus norvegicus | |
Diethyl-4-nitrophenylphosphate | irreversibly inactivated | Rattus norvegicus | |
diisopropyl fluorophosphate | irreversibly inactivated | Rattus norvegicus | |
F- | little effect on the activity | Rattus norvegicus | |
Hg2+ | little effect on the activity | Rattus norvegicus | |
additional information | not affected by detergents, i.e. Triton X-100, Triton CF-54, saponin; not affected by EDTA | Rattus norvegicus | |
p-chloromercuribenzoate | little effect on the activity | Rattus norvegicus | |
Phenylglyoxal | irreversibly inactivated | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.8 | - |
N-acetyl-9-O-acetylneuraminic acid | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no divalent cation requirement | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36400 | - |
1 * 36400 + 1* 30300, SDS-PAGE | Rattus norvegicus |
62800 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | O-acetyl esters | ? | - |
? | |
additional information | Rattus norvegicus | specific for sialic acids and selectively cleaves acetyl groups at the 9-position. it deacetylates di-O-acetyl- and tri-O-acetyl-N-acetylneuraminic acids by first cleaving the O-acetyl ester at the 9-position. The 7- and 8-acetylesters then undergo spontaneous migration to the 9-position, where they can be cleaved | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | contains N-linked oligosaccarides, after treatment with peptide N-glycosidase decrease of 10000 Da in the apparent molecular weight, endoglycosidase H causes a much smaller shift | Rattus norvegicus |
side-chain modification | glycoprotein, linked with N-oligosaccharides, LSE, cleaved by endo-beta-N-acetylglycosaminidase H and peptide:N-glycosidase F | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
3000fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | almost exclusively the nonglycosylated form of the enzyme | Rattus norvegicus | - |
colonic mucosa | high levels of activity of both forms of the enzyme, nonglycosylated and glycosylated | Rattus norvegicus | - |
heart | almost exclusively the nonglycosylated form of the enzyme | Rattus norvegicus | - |
kidney | high levels of activity of both forms, nonglycosylated and glycosylated | Rattus norvegicus | - |
liver | acetone powder, equal amounts of the glycosylated and nonglycosylated form of the enzyme | Rattus norvegicus | - |
skeletal muscle | only the glycosylated form of the enzyme | Rattus norvegicus | - |
testis | high levels of activity of both forms, nonglycosylated and glycosylated | Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
4°C, concentrated purified enzyme, stable for at least 1 month | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-O-acetylthymidine + H2O | 8% of the activity compared to N-acetyl-9-O-acetylneuraminate | Rattus norvegicus | thymidine + acetate | - |
? | |
4-methylumbelliferyl butyrate + H2O | less than 5% of the activity compared to 4-methylumbelliferyl acetate at pH 5.5 | Rattus norvegicus | 4-methylumbelliferone + butyrate | - |
? | |
4-nitrophenyl acetate + H2O | 72% of the activity compared to 4-methylumbelliferyl acetate at pH 5.5 | Rattus norvegicus | 4-nitrophenol + acetate | - |
? | |
alpha-naphthyl acetate + H2O | 65% of the activity compared to 4-methylumbelliferyl acetate at pH 5.5 | Rattus norvegicus | 1-naphthol + acetate | - |
? | |
additional information | strict substrate specificity for O-acetylated acids and a broad specificity for small synthetic esters, little or no activity against natural acetylated compounds other than sialic acids | Rattus norvegicus | ? | - |
? | |
additional information | O-acetyl esters | Rattus norvegicus | ? | - |
? | |
additional information | specific for sialic acids and selectively cleaves acetyl groups at the 9-position. it deacetylates di-O-acetyl- and tri-O-acetyl-N-acetylneuraminic acids by first cleaving the O-acetyl ester at the 9-position. The 7- and 8-acetylesters then undergo spontaneous migration to the 9-position, where they can be cleaved | Rattus norvegicus | ? | - |
? | |
N-acetyl-9-O-acetylneuraminate + H2O | - |
Rattus norvegicus | N-acetylneuraminate + acetate | - |
? | |
O-acetylserine + H2O | less than 5% of the activity compared to N-acetyl-9-O-acetylneuraminate | Rattus norvegicus | serine + acetate | - |
? | |
triacetin + H2O | 26% of the activity compared to N-acetyl-9-O-acetylneuraminate | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 36400 + 1* 30300, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
sialic acid O-acetylesterase | - |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
40% activity is lost in 20 h | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5: 16% of the activity, pH 8: 80% of the activity | Rattus norvegicus |