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Literature summary for 3.1.1.53 extracted from

  • Schauer, R.; Reuter, G.; Stoll, S.; Posadas del Rio, F.; Herrler, G.; Klenk, H.D.
    Isolation and characterization of sialate 9(4)-O-acetylesterase from influenza C virus (1988), Biol. Chem. Hoppe-Seyler, 369, 1121-1130.
    View publication on PubMed
Search for more literature for 3.1.1.53

Inhibitors

Inhibitors Comment Organism Structure
Diethyl-4-nitrophenylphosphate 50% inhibition at 0.24 mM influenza C virus
diisopropyl fluorophosphate 50% inhibition at 0.01 mM influenza C virus
physostigmine 25% inhibition at 10 mM influenza C virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 4-yethylumbelliferyl acetate
-
 influenza C virus
0.8
-
 N-acetyl-9-O-acetylneuraminic acid
-
 influenza C virus
0.9
-
 N-acetyl-9-O-lactoylneuraminic acid
-
 influenza C virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 1 * 57000 + 1* 24000, SDS-PAGE influenza C virus
80000
-
 SDS PAGE under non-reducing conditions influenza C virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-O-acetylneuraminate + H2O influenza C virus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
influenza C virus
-
-
-
influenza C virus Johannesburg/1/66
-
-
-

Purification (Commentary)

Purification (Comment) Organism
180fold enrichment influenza C virus

Storage Stability

Storage Stability Organism
-80°C, in lyophilized form, no loss of activity influenza C virus
37°C, in the presence of bovine serum albumin, decrease of 50% of the activity within 2 h influenza C virus
4°C, Tris-HCl, pH7.8, 2 mM mercaptoethanol, 90% loss of activity after 1 day, addition of 15 mM bovine serum albumin prevents loss during 7 days influenza C virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-2,3-didehydro-N-acetyl-9-O-acetylneuraminate + H2O 37% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus ?
-
 ?
4-methylumbelliferyl acetate + H2O
-
 influenza C virus 4-methylumbelliferone + acetate
-
 ?
4-methylumbelliferyl acetate + H2O
-
 influenza C virus Johannesburg/1/66 4-methylumbelliferone + acetate
-
 ?
4-methylumbelliferyl butyrate + H2O 14% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus 4-methylumbelliferone + butyrate
-
 ?
4-nitrophenyl acetate + H2O 3500% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O 3500% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus Johannesburg/1/66 4-nitrophenol + acetate
-
 ?
alpha-naphthyl acetate + H2O 2200% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus 1-naphthol + acetate
-
 ?
bovine submandibular gland mucin + H2O 30% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus ?
-
 ?
N-acetyl-4-O-acetylneuraminate + H2O 3% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus N-acetylneuraminate + acetate
-
 ?
N-acetyl-4-O-acetylneuraminate + H2O 3% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus Johannesburg/1/66 N-acetylneuraminate + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate + H2O
-
 influenza C virus N-acetylneuraminate + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate + H2O
-
 influenza C virus Johannesburg/1/66 N-acetylneuraminate + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate beta-methylglycoside + H2O 70% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus N-acetylneuraminate beta-methylglycoside + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate lactose + H2O 96% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus N-acetylneuraminate lactose + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate lactose + H2O 96% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus Johannesburg/1/66 N-acetylneuraminate lactose + acetate
-
 ?
N-acetyl-9-O-lactoylneuraminic acid + H2O
-
 influenza C virus 9-O-lactoylneuraminic acid + acetate
-
 ?
N-acetyl-O-acetylneuraminate + H2O
-
 influenza C virus ?
-
 ?
rat serum glycoprotein + H2O 90% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 57000 + 1* 24000, SDS-PAGE influenza C virus

Synonyms

Synonyms Comment Organism
9(4)-O-acetylesterase
-
 influenza C virus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
96
-
 in the presence of bovine serum albumin, loss of activity within 1 min influenza C virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 influenza C virus