Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 50 mM sodium-citrate buffer, pH 4.5 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
D17S/L162R | the mutant shows 2.2fold increased thioesterase activity compared to the wild type | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.78 | - |
1-lauryl-glycerophosphocholine | at pH 8.0 and 37°C | Pseudomonas aeruginosa | |
2.04 | - |
1-palmitoyl-glycerophosphocholine | at pH 8.0 and 37°C | Pseudomonas aeruginosa | |
7.22 | - |
1-hexyl-glycerophosphocholine | at pH 8.0 and 37°C | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hexyl-glycerophosphocholine + H2O | - |
Pseudomonas aeruginosa | hexanoate + glycerophosphocholine | - |
? | |
1-lauryl-glycerophosphocholine + H2O | - |
Pseudomonas aeruginosa | laurate + glycerophosphocholine | - |
? | |
1-oleoyl-glycerophosphocholine + H2O | - |
Pseudomonas aeruginosa | oleate + glycerophosphocholine | - |
? | |
1-palmitoyl-glycerophosphocholine + H2O | - |
Pseudomonas aeruginosa | palmitate + glycerophosphocholine | - |
? | |
1-stearoyl-glycerophosphocholine + H2O | - |
Pseudomonas aeruginosa | stearate + glycerophosphocholine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
lysophospholipase A | - |
Pseudomonas aeruginosa |
TesA | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
921.1 | - |
1-palmitoyl-glycerophosphocholine | at pH 8.0 and 37°C | Pseudomonas aeruginosa | |
1016 | - |
1-hexyl-glycerophosphocholine | at pH 8.0 and 37°C | Pseudomonas aeruginosa | |
1975 | - |
1-lauryl-glycerophosphocholine | at pH 8.0 and 37°C | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme exerts important functions in the homeostasis of phospholipids or detoxification of lysophospholipids | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
1-hexyl-glycerophosphocholine | at pH 8.0 and 37°C | Pseudomonas aeruginosa | |
450 | - |
1-palmitoyl-glycerophosphocholine | at pH 8.0 and 37°C | Pseudomonas aeruginosa | |
1100 | - |
1-lauryl-glycerophosphocholine | at pH 8.0 and 37°C | Pseudomonas aeruginosa |