Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
in complex with the tRNA CCA-acceptor-TpsiC domain of tRNAAla, sitting drop vapor diffusion method, using 100 mM sodium acetate buffer (pH 5.2), 20% (w/v) 1,4-butanediol and 30 mM glycyl-glycylglycine, at 20°C | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H188A | the mutation results in a 5.4fold decrease in the kcat/Km value compared to the wild type enzyme | Escherichia coli |
N185A | the mutation results in a 5.7fold decrease in the kcat/Km value compared to the wild type enzyme | Escherichia coli |
N185A/H188A | the mutation results in a 7.7fold decrease in the kcat/Km value compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00471 | - |
N-acetyl-Ala-tRNA(Ala) | wild type enzyme, in 20 mM HEPES-KOH (pH 7.6), 10 mM MgCl2, at 28°C | Escherichia coli | |
0.0134 | - |
N-acetyl-Ala-tRNA(Ala) | mutant enzyme N185A, in 20 mM HEPES-KOH (pH 7.6), 10 mM MgCl2, at 28°C | Escherichia coli | |
0.0171 | - |
N-acetyl-Ala-tRNA(Ala) | mutant enzyme H188A, in 20 mM HEPES-KOH (pH 7.6), 10 mM MgCl2, at 28°C | Escherichia coli | |
0.0269 | - |
N-acetyl-Ala-tRNA(Ala) | mutant enzyme N185A/H188A, in 20 mM HEPES-KOH (pH 7.6), 10 mM MgCl2, at 28°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-substituted aminoacyl-tRNA + H2O | Escherichia coli | - |
N-substituted amino acid + tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7D1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelating column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-Ala-tRNA(Ala) + H2O | - |
Escherichia coli | N-acetyl-Ala + tRNA(Ala) | - |
? | |
N-substituted aminoacyl-tRNA + H2O | - |
Escherichia coli | N-substituted amino acid + tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptidyl-tRNA hydrolase | - |
Escherichia coli |
PTH | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.8 | - |
N-acetyl-Ala-tRNA(Ala) | mutant enzyme N185A, in 20 mM HEPES-KOH (pH 7.6), 10 mM MgCl2, at 28°C | Escherichia coli | |
7.93 | - |
N-acetyl-Ala-tRNA(Ala) | mutant enzyme H188A, in 20 mM HEPES-KOH (pH 7.6), 10 mM MgCl2, at 28°C | Escherichia coli | |
8.7 | - |
N-acetyl-Ala-tRNA(Ala) | mutant enzyme N185A/H188A, in 20 mM HEPES-KOH (pH 7.6), 10 mM MgCl2, at 28°C | Escherichia coli | |
11.7 | - |
N-acetyl-Ala-tRNA(Ala) | wild type enzyme, in 20 mM HEPES-KOH (pH 7.6), 10 mM MgCl2, at 28°C | Escherichia coli |