Literature summary for 3.1.1.29 extracted from

Kumar, A.; Singh, N.; Yadav, R.; Kumar, R.P.; Sharma, S.; Arora, A.; Singh, T.P.
Crystal structure of peptidyl-tRNA hydrolase from Mycobacterium smegmatis reveals novel features related to enzyme dynamics (2012), Int. J. Biochem. Mol. Biol., 3, 58-69.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(lambdaDE3) cells	Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 30% (w/v) PEG-1500 and 10% (v/v) isopropanol in 100 mM HEPES buffer, pH 6.5	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21000	-	1 * 21000, SDS-PAGE	Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-substituted aminoacyl-tRNA + H2O	Mycolicibacterium smegmatis	-	N-substituted amino acid + tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex G-75 gel filtration	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-substituted aminoacyl-tRNA + H2O	-	Mycolicibacterium smegmatis	N-substituted amino acid + tRNA	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 21000, SDS-PAGE	Mycolicibacterium smegmatis

Synonyms

Synonyms	Comment	Organism
peptidyl-tRNA hydrolase	-	Mycolicibacterium smegmatis
PTH	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)