Literature summary for 3.1.1.29 extracted from

Goodall, J.J.; Chen, G.J.; Page, M.G.
Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase (2004), Biochemistry, 43, 4583-4591.

Search for more literature for 3.1.1.29

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure at 1.3 A resolution	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D93N	4% of wild-type kcat	Escherichia coli
H20N	no activity	Escherichia coli
H20Q	no activity	Escherichia coli
K103Q	54% of wild-type kcat	Escherichia coli
K103R	68% of wild-type kcat	Escherichia coli
K103S	28% of wild-type kcat	Escherichia coli
K105Q	20% of wild-type kcat	Escherichia coli
K105R	26% of wild-type kcat	Escherichia coli
K105S	16% of wild-type kcat	Escherichia coli
K113Q	98% of wild-type kacat	Escherichia coli
M67E	0.5% of wild-type kcat	Escherichia coli
N10D	0.05% of wild-type kcat	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
diethyldicarbonate	0.5 mM, 90% inactivation after 10 min, activity can be recovered to 41% of initial activity by treatment wit 200 mM hydroxylamine	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0055	-	Oregon Green-methionine-tRNA	25°C, pH 7.5	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-substituted aminoacyl-tRNA + H2O	Escherichia coli	-	N-substituted amino acid + tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant Pth	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-substituted aminoacyl-tRNA + H2O	-	Escherichia coli	N-substituted amino acid + tRNA	-	?
Oregon Green-methionine-tRNA + H2O	-	Escherichia coli	Oregon Green-methionine + tRNA	-	?

Synonyms

Synonyms	Comment	Organism
PTH	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9.3	-	Oregon Green-methionine-tRNA	25°C, pH 7.5	Escherichia coli

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Release 2023.1 (January 2023)