Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 38.9% residual activity in the presence of 50 mM Ca2+ | Agarophyton vermiculophyllum | |
Mg2+ | 90.7% residual activity in the presence of 50 mM Mg2+ | Agarophyton vermiculophyllum | |
Mn2+ | 12.2% residual activity in the presence of 50 mM Mn2+ | Agarophyton vermiculophyllum | |
Zn2+ | 16.3% residual activity in the presence of 50 mM Zn 2+ | Agarophyton vermiculophyllum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 130% relative activity in the presence of 5 mM Ca2+ | Agarophyton vermiculophyllum | |
Mg2+ | 151% relative activity in the presence of 5 mM Mg2+ | Agarophyton vermiculophyllum | |
Mn2+ | 128% relative activity in the presence of 10 mM Mn2+ | Agarophyton vermiculophyllum | |
Zn2+ | 128% relative activity in the presence of 1 mM Zn2+ | Agarophyton vermiculophyllum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 20000, SDS-PAGE | Agarophyton vermiculophyllum |
40000 | - |
gel filtration | Agarophyton vermiculophyllum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Agarophyton vermiculophyllum | the enzyme hydrolyzes the membrane lipids to release various saturated and unsaturated fatty acids, including arachidonic acid as substrate for prostaglandin synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarophyton vermiculophyllum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Toyopearl 650 M colum chromatography, and HW-55F gel filtration | Agarophyton vermiculophyllum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000194 | - |
crude extract | Agarophyton vermiculophyllum |
0.00367 | - |
after 18.9fold purification | Agarophyton vermiculophyllum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-di-O-dodecanoyl-3-O-beta-D-galactopyranosyl-sn-glycerol + H2O | - |
Agarophyton vermiculophyllum | 2,3-dihydroxypropyl beta-D-galactopyranoside + dodecanoate | - |
? | |
3-O-(6-O-alpha-D-galactopyranosyl-beta-D-galactopyranosyl)-1,2-di-O-dodecanoyl-sn-glycerol + H2O | - |
Agarophyton vermiculophyllum | 2,3-dihydroxypropyl 6-O-beta-D-galactopyranosyl-beta-D-galactopyranoside + 2 dodecanoate | - |
? | |
digalactosyldiacylglycerol + H2O | - |
Agarophyton vermiculophyllum | digalactosylglycerol + fatty acid | - |
? | |
monogalactosyldiacylglycerol + H2O | high activity | Agarophyton vermiculophyllum | galactosylglycerol + fatty acid | - |
? | |
additional information | the enzyme hydrolyzes the membrane lipids to release various saturated and unsaturated fatty acids, including arachidonic acid as substrate for prostaglandin synthesis | Agarophyton vermiculophyllum | ? | - |
? | |
phosphatidylcholine + H2O | highest activity | Agarophyton vermiculophyllum | ? | - |
? | |
phosphatidylglycerol + H2O | - |
Agarophyton vermiculophyllum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 20000, SDS-PAGE | Agarophyton vermiculophyllum |
Synonyms | Comment | Organism |
---|---|---|
glycerolipid acyl-hydrolase | - |
Agarophyton vermiculophyllum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Agarophyton vermiculophyllum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | the enzymatic activity increases gradually from 10 to 30°C, drastically increases at 37°C and then continuously decreases over 37°C | Agarophyton vermiculophyllum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Agarophyton vermiculophyllum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Agarophyton vermiculophyllum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
the enzyme is inactive above pH 10.0 | Agarophyton vermiculophyllum |