Information on EC 3.1.1.26 - galactolipase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.1.26
-
RECOMMENDED NAME
GeneOntology No.
galactolipase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1,2-diacyl-3-beta-D-galactosyl-sn-glycerol + 2 H2O = 3-beta-D-galactosyl-sn-glycerol + 2 carboxylates
show the reaction diagram
also acts on 2,3-di-O-acyl-1-O-(6-O-alpha-D-galactosyl)-D-glycerol, and phosphatidylcholine and other phospholipids
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerolipid metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
1,2-diacyl-3-beta-D-galactosyl-sn-glycerol acylhydrolase
Also acts on 2,3-di-O-acyl-1-O-(6-O-alpha-D-galactosyl-beta-D-galactosyl)-D-glycerol, and phosphatidylcholine and other phospholipids.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-40-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
L. cv. Goliat
-
-
Manually annotated by BRENDA team
L. napus, cv. Gorczanski, no activity in cvs. Jet Neuf and Start
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
L. cv. Wilanowska, cucumber
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
L. cvs. Afus, Aga and Remik
-
-
Manually annotated by BRENDA team
L. cv Verko, alfalfa
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Bos taurus
-
-
-
Manually annotated by BRENDA team
no activity in Brassica rapa
L. var. silvestris cv. Ludowy
-
-
Manually annotated by BRENDA team
no activity in Capra hircus
-
-
-
Manually annotated by BRENDA team
no activity in Cucumis melo
cv. Kubicki, melon
-
-
Manually annotated by BRENDA team
no activity in Cucurbita maxima
cv. Bambino, pumpkin
-
-
Manually annotated by BRENDA team
no activity in Cucurbita pepo
convar. giromontina cv. Ciemnozielona, squash
-
-
Manually annotated by BRENDA team
no activity in Glycine max
-
-
-
Manually annotated by BRENDA team
no activity in Hordeum vulgare
L. var. Popiel
-
-
Manually annotated by BRENDA team
no activity in Lactuca sativa
var. Amplus
-
-
Manually annotated by BRENDA team
no activity in Meleagris gallopavo
-
-
-
Manually annotated by BRENDA team
no activity in Ovis aries
-
-
-
Manually annotated by BRENDA team
no activity in Passiflora edulis
Sims
-
-
Manually annotated by BRENDA team
no activity in Passiflora edulis Sims
Sims
-
-
Manually annotated by BRENDA team
no activity in Passiflora quadrangularis
L.
-
-
Manually annotated by BRENDA team
no activity in Passiflora quadrangularis L.
L.
-
-
Manually annotated by BRENDA team
no activity in Solanum x ajanhuiri
-
-
-
Manually annotated by BRENDA team
no activity in Struthio camelus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Pisum arvense
Pisum arvense L.
L.
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
L., wheat
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1(3)-monoolein + H2O
?
show the reaction diagram
-
-
-
?
1,2(2,3)-diolein + H2O
?
show the reaction diagram
-
-
-
?
1,2-di-O-dodecanoyl-3-O-beta-D-galactopyranosyl-sn-glycerol + H2O
2,3-dihydroxypropyl beta-D-galactopyranoside + dodecanoate
show the reaction diagram
1,2-di-O-dodecanoyl-3-O-beta-D-galactopyranosyl-sn-glycerol + H2O
?
show the reaction diagram
-
-
-
-
?
1,2-diacyl-3-(6-O-alpha-D-Gal-beta-D-Gal)glycerol + H2O
2,3-dihydroxypropyl 6-O-alpha-D-galactopyranosyl-beta-D-galactopyranoside + 2 fatty acid
show the reaction diagram
-
-
-
-
?
1,2-diacyl-3-O-(6-O-alpha-D-galactosyl-beta-D-galactosyl)-sn-glycerol + H2O
2-acyl-3-O-(6-O-alpha-D-galactosyl-beta-D-galactosyl)-sn-glycerol + fatty acid
show the reaction diagram
1,2-diacyl-3-O-beta-D-galactosyl-sn-glycerol + H2O
? + fatty acid
show the reaction diagram
1,2-dicaprine + H2O
?
show the reaction diagram
-
-
-
-
?
1,2-didodecanoyl phosphatidylglycerol + 2 H2O
2 dodecanoate + sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
1,2-dioctanoyl 3-monogalactosyl glycerol + H2O
?
show the reaction diagram
2,3-di-O-acyl-1-O-(beta-D-galactosyl)-D-glycerol + H2O
?
show the reaction diagram
-
-
-
-
?
3-O-(6-O-alpha-D-galactopyranosyl-beta-D-galactopyranosyl)-1,2-di-O-dodecanoyl-sn-glycerol + 2 H2O
2,3-dihydroxypropyl 6-O-beta-D-galactopyranosyl-beta-D-galactopyranoside + 2 dodecanoate
show the reaction diagram
-
-
-
?
3-O-(6-O-alpha-D-galactopyranosyl-beta-D-galactopyranosyl)-1,2-di-O-dodecanoyl-sn-glycerol + H2O
2,3-dihydroxypropyl 6-O-beta-D-galactopyranosyl-beta-D-galactopyranoside + 2 dodecanoate
show the reaction diagram
3-O-beta-D-galactopyranosyl-1,2-di-O-dodecanoyl-sn-glycerol + fatty acid + H2O
2,3-dihydroxypropyl-beta-D-galactopyranoside + dodecanoate
show the reaction diagram
-
-
-
-
?
acylsterylglucoside + H2O
?
show the reaction diagram
-
500% of the activity with monogalactosyldiacylglycerol
-
-
?
digalactosyldiacylglycerol + H2O
?
show the reaction diagram
digalactosyldiacylglycerol + H2O
digalactosylglycerol + fatty acid
show the reaction diagram
digalactosyldilinolenin + H2O
digalactosylglycerol + linolenic acid
show the reaction diagram
-
-
-
-
?
digalactosylmonoacylglycerol + H2O
digalactosylglycerol + fatty acid
show the reaction diagram
L-alpha-phosphatidylcholine + H2O
?
show the reaction diagram
lysophosphatidylcholine + H2O
?
show the reaction diagram
lysophosphatidylethanolamine + H2O
?
show the reaction diagram
lysophosphatidylglycerol + H2O
?
show the reaction diagram
monogalactosyl dioctanoylglycerol + H2O
galactosylglycerol + 2 octanoate
show the reaction diagram
-
-
-
-
?
monogalactosyldiacylglycerol + H2O
?
show the reaction diagram
monogalactosyldiacylglycerol + H2O
galactosylglycerol + fatty acid
show the reaction diagram
monogalactosyldilinolenin + H2O
monogalactosylglycerol + linolenic acid
show the reaction diagram
-
-
-
-
?
monogalactosylmonoacylglycerol + H2O
monogalactosylglycerol + fatty acid
show the reaction diagram
phosphatidic acid + H2O
?
show the reaction diagram
phosphatidylcholine + H2O
?
show the reaction diagram
phosphatidylcholine + H2O
lysophosphatidylcholine + fatty acid
show the reaction diagram
-
-
-
-
?
phosphatidylethanolamine + H2O
?
show the reaction diagram
phosphatidylglycerol + H2O
?
show the reaction diagram
phosphatidylinositol + H2O
?
show the reaction diagram
-
36% of the activity with monogalactosyldiacylglycerol
-
-
?
sulfoquinovosyldiaclglycerol + H2O
?
show the reaction diagram
triacylglycerol + H2O
?
show the reaction diagram
tributyrin + H2O
?
show the reaction diagram
trioctanoin + H2O
?
show the reaction diagram
triolein + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
digalactosyldiacylglycerol + H2O
?
show the reaction diagram
monogalactosyldiacylglycerol + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
130% relative activity in the presence of 5 mM Ca2+
Mg2+
-
151% relative activity in the presence of 5 mM Mg2+
Mn2+
-
128% relative activity in the presence of 10 mM Mn2+
Sodium dithionite
-
highest activity between 4-7 mM, above decrease of activation
Zn2+
-
128% relative activity in the presence of 1 mM Zn2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4,6-trifluoro-1,3,5-triazine
-
16% inhibition at 7 mM, 93% inhibition at 74 mM, 98% inhibition at 741 mM
2-mercaptoethanol
Ca2+
-
38.9% residual activity in the presence of 50 mM Ca2+
CaCl2
Cetyltrimethylammonium chloride
-
65% inhibition at 1 mM
cysteine
Digitonin
-
complete inhibition, concentration below critical micellar concentration
diisopropylfluorophosphate
dithioerythritol
-
13% inhibition with 13 mM
E600
-
in the absence of bile salts and colipase, the time required to obtain 50% inactivation of PLRP2 by E600 is 3 min, in the presence of 4 mM sodium taurodeoxycholate and colipase, the rate of inhibition of PLRP2 by E600 increases significantly and 50% inhibition is reached within 1 min
EDTA
-
no inhibition at 1 mM, 78% inhibition at 10 mM
glutathione
-
reduced, 48% inhibition 5-10 mM
methyl arachidonyl fluorophosphonate
-
30% residual activity at 0.4 mM
Mg2+
-
90.7% residual activity in the presence of 50 mM Mg2+
MgCl2
-
53% inhibition at 10 mM
Mn2+
-
12.2% residual activity in the presence of 50 mM Mn2+
N-Acetylimidazole
-
30% inhibition at 30fold mol per mol of enzyme, 48% inhibition at 465fold mol per mol of enzyme, complete inhibition at 180000fold mol per mol of enzyme
N-ethylmaleimide
-
20% inhibition at 30 mM, 39% inhibition at 60 mM
Triton X-100
Trypsin
-
1000 units, pH 7.6, phosphate buffer at 24C, completely inactivated
-
Zn2+
-
16.3% residual activity in the presence of 50 mM Zn 2+
Zwittergent 3-19
-
complete inhibition, concentration below critical micellar concentration
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sodium deoxycholate
-
stimulates the activity
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.31
digalactosyldiacylglycerol
-
-
7.8
digalactosyldilinolenin
-
-
-
0.119 - 0.65
monogalactosyldiacylglycerol
1.5
monogalactosyldilinolenin
-
-
-
0.53 - 1.7
phosphatidylcholine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 10
cysteine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000194
-
crude extract
0.00021
-
-
0.00059
-
-
0.00066
-
-
0.00367
-
after 18.9fold purification
0.33
Pisum arvense
-
-
0.71 - 0.72
-
cvs. Afus and Remik
0.82 - 0.98
-
-
1.25
-
cv. Aga
1.26
-
-
1.3
-
wild type enzyme, using 1,2(2,3)-diolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
1.5
-
-
2.8
-
-
3.4
-
mutant enzyme N336Q, using 1,2(2,3)-diolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
3.75
-
-
4.3
-
mutant enzyme N336Q, using triolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
5
-
wild type enzyme, using triolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
40.2
-
mutant enzyme N336Q, using trioctanoin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
43
-
wild type enzyme, using trioctanoin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
212
-
mutant enzyme N336Q, using tributyrin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
247
-
mutant enzyme N336Q, using 1(3)-monoolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
287
-
wild type enzyme, using tributyrin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
317
-
wild type enzyme, using 1(3)-monoolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37C, in the absence of colipase
1756
-
purified enzyme, using natural long chain 1,2-diacyl-3-O-(6-O-alpha-D-galactosyl-beta-D-galactosyl)-sn-glycerol as substrate, at 37C, pH not specified in the publication
1786
-
purified enzyme, using synthetic medium chain 1,2-diacyl-3-O-beta-D-galactosyl-sn-glycerol as substrate, at 37C, pH not specified in the publication
2441
-
purified enzyme, using natural long chain 1,2-diacyl-3-O-beta-D-galactosyl-sn-glycerol as substrate, at 37C, pH not specified in the publication
2976
-
purified enzyme, using synthetic medium chain 1,2-diacyl-3-O-(6-O-alpha-D-galactosyl-beta-D-galactosyl)-sn-glycerol as substrate, at 37C, pH not specified in the publication
3351
-
purified enzyme, using natural long chain 1,2-diacyl-3-O-(6-O-alpha-D-galactosyl-beta-D-galactosyl)-sn-glycerol as substrate, at 37C, pH not specified in the publication
4167
-
purified enzyme, using natural long chain 1,2-diacyl-3-O-beta-D-galactosyl-sn-glycerol as substrate, at 37C, pH not specified in the publication
4375
-
purified enzyme, using synthetic medium chain 1,2-diacyl-3-O-(6-O-alpha-D-galactosyl-beta-D-galactosyl)-sn-glycerol as substrate, at 37C, pH not specified in the publication
5420
-
purified enzyme, using synthetic medium chain 1,2-diacyl-3-O-beta-D-galactosyl-sn-glycerol as substrate, at 37C, pH not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
monogalactosyldiacylglycerol as substrate, at 35C, shift to pH 6.0 in the presence of Triton X-100
5.5 - 6
5.9
-
digalactosyldiacylglycerol as substrate
6
-
monogalactosyldiacylglycerol as substrate, at 35C, shift from pH 5.5 in the presence of Triton X-100
7.2
-
with both monogalactosyldiacylglycerol and digalactosyldiacylglycerol as substrates, Tris-maleate buffer in the presence of 1.5 mM
8.5
-
phosphatidylcholine as substrate, in Tris-HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
-
pH 4: 25% of the maximal activity, pH 9: 50% of the maximal activity
5 - 8
-
pH 5: about 10% of the maximal activity, pH 8: about 50% of the maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
maximally active after 10 min incubation
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 45
-
0C: 50% of the maximal activity, 45C: 40% of the maximal activity
0 - 20
10 - 50
-
the enzymatic activity increases gradually from 10 to 30C, drastically increases at 37C and then continuously decreases over 37C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.55
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
2 * 20000, SDS-PAGE
43480
calculated from amino acid sequence
50078
-
x * 50078, deglycoslyated protein, MALDI-TOF mass spectrometry
50080
-
mature PLRP2, calculated from amino acid sequence
50081
-
x * 50081, calculated from amino acid sequence
52820
-
glycosylated enzyme, LC-ESI-TOF mass spectrometry
52924
-
x * 52924, glycoslyated protein, MALDI-TOF mass spectrometry
70000
-
gel filtration
80000
-
gel filtration
90000
-
SDS-PAGE, gel filtration
110000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 40000, SDS-PAGE
homodimer
-
2 * 20000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method, using 1.17 M (NH4)2SO4, 0.1 M Na-cacodylate pH 5.5, 0.04 M NaCl for wild type enzyme, and 2.05 M ammonium sulfate, 0.1 M HEPES, pH 6.7 for mutant enzyme N336Q
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
the enzyme is inactive above pH 10.0
692412
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
incubation for 10 min at pH 7.5, 95% loss of activity, retains more thant 60% of its activity at pH 5.5
55
-
almost no loss of activity after 30 min
65
-
inactivated, pH 4 after 20 min, 80% of the activity retained by the same heating at pH 7.5
70
-
only little loss of activity after 30 min
80
-
complete loss of activity after 30 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing do not damage the activity
-
Sonication inactivates
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
Ethanol
additional information
-
no effect with ethyl ether, methanol, and chloroform
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, purified enzyme preparation, 3 months stable
-
4C, (NH4)2SO4-precipitation at pH-optimum, after 4 weeks 53.6% of the original activity left
-
reduced activity after 3-4 h storage on ice, though improved stability in the presence of bovine serum albumin
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
268fold
-
ammonium sulfate precipitation, DEAE-Toyopearl 650 M colum chromatography, and HW-55F gel filtration
-
HiTrap Q HP column chromatography
-
Superdex 75 gel filtration and Macro-Prep DEAE column chromatography
Superdex 75 gel filtration and Macro-Prep DEAE column chromatography; Superdex 75 gel filtration and Macro-Prep DEAE column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Aspergillus oryzae
-
expressed in baculovirus system
expressed in BTI-TN-5B1-4 cells
-
expressed in Pichia pastoris
-
expressed in SF9 insect cells (wild type enzyme) and in Pichia pastoris (mutant enzyme N336Q)
-
expression in Pichia pastoris or insect cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
feeding Epiphyas postvittana on a fatty acid-free artificial diet induces a large increase in galactolipase activity
-
switching Tineola bisselliella larvae to wool leads has no effect on the level of galactolipase activity
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N336Q
-
the mutant enzyme is not glycosylated and shows identical kinetic properties to the wild type enzyme
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis