Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli | Vulcanisaeta moutnovskia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.05-10 mM, nearly complete loss of activity | Vulcanisaeta moutnovskia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
gamma-(R)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
0.75 | - |
gamma-(S)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
0.93 | - |
5-butyl-4-methyldihydro-2(3H)-furanone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
1.95 | - |
gamma-(S)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
4.56 | - |
gamma-(R)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
11.57 | - |
gamma-butyrolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia | |
Co2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia | |
Mn2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia | |
Ni2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia | |
Zn2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Vulcanisaeta moutnovskia |
82000 | - |
gel filtration | Vulcanisaeta moutnovskia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vulcanisaeta moutnovskia | F0QXN6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Vulcanisaeta moutnovskia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-butyl-4-methyldihydro-2(3H)-furanone + H2O | i.e. whiskey lactone | Vulcanisaeta moutnovskia | 4-hydroxy-3-methyl-octanoate | - |
? | |
9-oxo-N-(2-oxotetrahydrofuran-3-yl)undecanamide + H2O | - |
Vulcanisaeta moutnovskia | 3-hydroxy-2-[(9-oxoundecanoyl)amino]propanoate | - |
? | |
gamma-(R)-caprolactone + H2O | slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone | Vulcanisaeta moutnovskia | 4-hydroxyhexanoate | - |
? | |
gamma-(R)-valerolactone + H2O | slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone | Vulcanisaeta moutnovskia | 4-hydroxypentanoate | - |
? | |
gamma-(S)-caprolactone + H2O | slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone | Vulcanisaeta moutnovskia | 4-hydroxyhexanoate | - |
? | |
gamma-(S)-valerolactone + H2O | slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone | Vulcanisaeta moutnovskia | 4-hydroxypentanoate | - |
? | |
gamma-butyrolactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxybutanoate | - |
? | |
N-(2-oxotetrahydrofuran-3-yl)nonanamide + H2O | - |
Vulcanisaeta moutnovskia | 3-hydroxy-2-(nonanoylamino)propanoate | - |
? | |
N-(2-oxotetrahydrofuran-3-yl)pentanamide + H2O | - |
Vulcanisaeta moutnovskia | 3-hydroxy-2-(pentanoylamino)propanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36000, SDS-PAGE | Vulcanisaeta moutnovskia |
Synonyms | Comment | Organism |
---|---|---|
Vmut2255 | - |
Vulcanisaeta moutnovskia |
VmutPLL | - |
Vulcanisaeta moutnovskia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | 70°C: about 60% of maximal activity, 90°C: about 50% of maximal activity, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
3 h, 30% loss of specific activity, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
80 | - |
half-life: 2 h, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
90 | - |
half-life: 26 min, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.89 | - |
gamma-(S)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
2.68 | - |
gamma-(S)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
2.79 | - |
gamma-butyrolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
3.04 | - |
gamma-(R)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
4.2 | - |
5-butyl-4-methyldihydro-2(3H)-furanone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
6.23 | - |
gamma-(R)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 30% of maximal activity, pH 9.0: about 80% of maximal activity, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
gamma-butyrolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
1.33 | - |
gamma-(R)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
1.38 | - |
gamma-(S)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
2.53 | - |
gamma-(S)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
4.54 | - |
5-butyl-4-methyldihydro-2(3H)-furanone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
5.56 | - |
gamma-(R)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia |