Information on EC 3.1.1.25 - 1,4-lactonase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.1.1.25
-
RECOMMENDED NAME
GeneOntology No.
1,4-lactonase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 1,4-lactone + H2O = a 4-hydroxyacid
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic ester hydrolysis
-
-
hydrolysis of carboxylic ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-galactose degradation II
-
-
Galactose metabolism
-
-
Ascorbate and aldarate metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
1,4-lactone hydroxyacylhydrolase
The enzyme is specific for 1,4-lactones with 4-8 carbon atoms. It does not hydrolyse simple aliphatic esters, acetylcholine, sugar lactones or substituted aliphatic lactones, e.g. 3-hydroxy-4-butyrolactone; requires Ca2+.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-38-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subsp. Kurstaki
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the presence of a particular amino acid residue at position 192 differentially alters the effect of the H115W substitution, and the H115 residue is not always needed for the lactonase and arylesterase activities of the enzyme. The amino acid residues at position 192 and 115 act in conjunction in modulating the hydrolytic activities of the enzyme
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1R,5S)-oxabicyclooctenone + H2O
?
show the reaction diagram
(1S,5R)-oxabicyclooctenone + H2O
?
show the reaction diagram
(2S,4RS)-2-hydroxy-4-butyrolactone + H2O
(S)-2-hydroxy-4-phenylbutyrate
show the reaction diagram
-
-
-
-
?
(R)-alpha-hydroxy-gamma-butyrolactone + H2O
(R)-2-hydroxybutyric acid
show the reaction diagram
enzyme variant Q192: 0.88% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.95% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
(R)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O
?
show the reaction diagram
enzyme variant Q192: 1.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.29% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
(S)-alpha-hydroxy-gamma-butyrolactone + H2O
(S)-2-hydroxybutyric acid
show the reaction diagram
enzyme variant Q192: 8.14% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 19.6% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
(S)-beta-hydroxy-gamma-butyrolactone + H2O
(S)-3-hydroxybutyric acid
show the reaction diagram
enzyme variant Q192: 0.6% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 0.76% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
(S)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O
?
show the reaction diagram
enzyme variant Q192: 0.78% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 1.12% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
1,3-benzoxazol-2-one + H2O
?
show the reaction diagram
-
-
-
-
?
2-bromobutyrolactone + H2O
?
show the reaction diagram
less than 1% activity compared to DL-pantoyl lactone
-
-
?
2-coumaranone + H2O
(2-hydroxyphenyl)acetic acid
show the reaction diagram
2-coumaranone + H2O
?
show the reaction diagram
2-hydroxybutyrolactone + H2O
?
show the reaction diagram
2450% activity compared to DL-pantoyl lactone
-
-
?
3,4-dihydrocoumarin + H2O
?
show the reaction diagram
3-oxo-decanoyl-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
show the reaction diagram
3-oxo-octanoyl-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
show the reaction diagram
4-(1-propenyloxymethyl)-1,3-dioxolan-2-one + H2O
?
show the reaction diagram
enzyme variant Q192: 2.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 2.52% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
4-butyrolactone + H2O
?
show the reaction diagram
less than 1% activity compared to DL-pantoyl lactone
-
-
?
4-phenyl-2-hydroxyl-4-butyrolactone + H2O
?
show the reaction diagram
22% activity compared to DL-pantoyl lactone
-
-
?
5-(thiobutyl)butyrolactone + H2O
?
show the reaction diagram
5-(thioethyl)butyrolactone + H2O
?
show the reaction diagram
the lactonase and the esterase activity of PON1 is mediated by the His115-His134 dyad
-
-
?
5-butyl-4-methyldihydro-2(3H)-furanone + H2O
4-hydroxy-3-methyl-octanoate
show the reaction diagram
-
i.e. whiskey lactone
-
-
?
5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoic acid 1,5-lactone + H2O
5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoic acid
show the reaction diagram
-
-
-
?
5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 1,5-lactone + H2O
5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid
show the reaction diagram
-
-
-
?
5-thiobutyl-butyro-1,4-lactone + H2O
?
show the reaction diagram
-
-
-
-
?
7-O-diethyl phosphoryl 3-cyano 4-methyl 7-hydroxycoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
7-oxo-N-(2-oxotetrahydrofuran-3-yl)nonanamide + H2O
3-hydroxy-2-[(7-oxononanoyl)amino]propanoic acid
show the reaction diagram
9-oxo-N-(2-oxotetrahydrofuran-3-yl)undecanamide + H2O
3-hydroxy-2-[(9-oxoundecanoyl)amino]propanoate
show the reaction diagram
-
-
-
-
?
9-oxo-N-(2-oxotetrahydrofuran-3-yl)undecanamide + H2O
3-hydroxy-2-[(9-oxoundecanoyl)amino]propanoic acid
show the reaction diagram
alpha-angelica lactone + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-angelicalactone + H2O
4-hydroxy-pent-3-enoic acid
show the reaction diagram
-
-
-
-
alpha-angelicalactone + H2O
4-hydroxypent-3-enoic acid
show the reaction diagram
-
-
-
-
alpha-angelicalactone + H2O
?
show the reaction diagram
enzyme variant Q192: 19.9% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
alpha-bromo-gamma-butyrolactone + H2O
2-bromobutyric acid
show the reaction diagram
enzyme variant Q192: 47.2% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 40.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
alpha-hydroxy-beta,beta-dimethyl-gamma-butyrolactone + H2O
2,4-dihydroxy-3,3-dimethylbutanoate
show the reaction diagram
-
-
-
-
?
alpha-hydroxy-gamma-butyrolactone + H2O
2,4-dihydroxybutanoate
show the reaction diagram
-
-
-
-
?
beta-butyrolactone + H2O
3-hydroxybutanoate
show the reaction diagram
-
-
-
-
?
beta-hydroxy-gamma-butyrolactone + H2O
3,4-dihydroxybutanoate
show the reaction diagram
-
-
-
-
?
beta-hydroxybutyrolactone + H2O
?
show the reaction diagram
canrenone + H2O
?
show the reaction diagram
not hydrolyzed by PON1 (P27169 (UniProt)) and PON2 (Q15165 (UniProt))
-
-
?
D-pantolactone + H2O
D-pantoic acid
show the reaction diagram
-
-
-
-
-
delta-decanolactone + H2O
5-hydroxydecanoic acid
show the reaction diagram
delta-decanolactone + H2O
?
show the reaction diagram
-
-
-
-
?
delta-dodecalactone + H2O
5-hydroxydecanoate
show the reaction diagram
-
-
-
-
?
delta-dodecanolactone + H2O
5-hydroxydodecanoate
show the reaction diagram
-
-
-
?
delta-dodecanolactone + H2O
?
show the reaction diagram
-
-
-
-
?
delta-hexalactone + H2O
5-hydroxyhexanoic acid
show the reaction diagram
-
-
-
?
delta-hexalactone + H2O
?
show the reaction diagram
-
-
-
-
?
delta-nonalactone + H2O
5-hydroxynonanoate
show the reaction diagram
delta-nonalactone + H2O
5-hydroxynonanoic acid
show the reaction diagram
delta-nonalactone + H2O
?
show the reaction diagram
-
-
-
-
?
delta-tetradecanolactone + H2O
5-hydroxytetradecanoic acid
show the reaction diagram
-
-
-
?
delta-undecalactone + H2O
5-hydroxydecanoate
show the reaction diagram
-
-
-
-
?
delta-undecanolactone + H2O
5-hydroxyundecanoate
show the reaction diagram
-
-
-
?
delta-undecanolactone + H2O
5-hydroxyundecanoic acid
show the reaction diagram
-
-
-
?
delta-undecatiolactone + H2O
?
show the reaction diagram
-
-
-
-
?
delta-valerolactone + H2O
5-hydroxypentanoic acid
show the reaction diagram
delta-valerolactone + H2O
?
show the reaction diagram
dihydrocoumarin + H2O
3-(2-hydroxyphenyl)-propionic acid
show the reaction diagram
dihydrocoumarin + H2O
3-(2-hydroxyphenyl)propanoic acid
show the reaction diagram
DL-3-oxo-hexanoyl-homoserine lactone + H2O
DL-3-oxo-hexanoyl-homoserine
show the reaction diagram
-
-
-
?
DL-3-oxo-hexanoylhomoserine lactone + H2O
DL-3-oxo-hexanoylhomoserine
show the reaction diagram
-
-
-
?
DL-dodecanoyl-homoserine lactone + H2O
DL-dodecanoyl-homoserine
show the reaction diagram
-
-
-
?
DL-dodecanoylhomoserine lactone + H2O
DL-dodecanoylhomoserine
show the reaction diagram
-
-
-
?
DL-heptanoyl-homoserine lactone + H2O
DL-heptanoyl-homoserine
show the reaction diagram
-
-
-
?
DL-heptanoylhomoserine lactone + H2O
DL-heptanoylhomoserine
show the reaction diagram
-
-
-
?
DL-pantoyl lactone + H2O
pantoic acid
show the reaction diagram
-
-
-
-
?
DL-pantoyllactone + H2O
pantoic acid
show the reaction diagram
100% activity
-
-
?
DL-tetradecanoyl-homoserine lactone + H2O
DL-tetradecanoyl-homoserine
show the reaction diagram
-
-
-
?
DL-tetradecanoylhomoserine lactone + H2O
DL-tetradecanoyl-homoserine
show the reaction diagram
-
-
-
?
DL-tetradecanoylhomoserine lactone + H2O
DL-tetradecanoylhomoserine
show the reaction diagram
-
-
-
?
dodecanoic-1,4-lactone + H2O
4-hydroxydodecanoate
show the reaction diagram
-
-
-
?
dodecanoic-1,5-lactone + H2O
5-hydroxydodecanoate
show the reaction diagram
-
-
-
?
dodecanoic-delta-lactone + H2O
dodecanoate
show the reaction diagram
dodecanoic-gamma-lactone + H2O
dodecanoate
show the reaction diagram
-
-
-
?
epsilon-caprolactone + H2O
5-hydroxyhexanoate
show the reaction diagram
-
-
-
?
epsilon-caprolactone + H2O
5-hydroxyhexanoic acid
show the reaction diagram
enzyme variant Q192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 25.7% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
epsilon-caprolactone + H2O
?
show the reaction diagram
ethyl acetate + H2O
?
show the reaction diagram
less than 1% activity compared to DL-pantoyl lactone
-
-
?
ethyl acetate + H2O
ethanol + acetate
show the reaction diagram
-
-
-
-
?
gamma -nonalactone + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-(R)-caprolactone + H2O
4-hydroxyhexanoate
show the reaction diagram
-
slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone
-
-
?
gamma-(R)-valerolactone + H2O
4-hydroxypentanoate
show the reaction diagram
-
slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone
-
-
?
gamma-(S)-caprolactone + H2O
4-hydroxyhexanoate
show the reaction diagram
-
slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone
-
-
?
gamma-(S)-valerolactone + H2O
4-hydroxypentanoate
show the reaction diagram
-
slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone
-
-
?
gamma-butyrolactone + H2O
4-hydroxy-butyric acid
show the reaction diagram
-
-
-
?
gamma-butyrolactone + H2O
4-hydroxybutanoate
show the reaction diagram
gamma-butyrolactone + H2O
4-hydroxybutanoic acid
show the reaction diagram
-
-
-
-
?
gamma-butyrolactone + H2O
4-hydroxybutyric acid
show the reaction diagram
-
-
-
?
gamma-butyrolactone + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-butyrolactone + H2O
butanoate
show the reaction diagram
-
-
-
?
gamma-butyrolactone + H2O
butyric acid
show the reaction diagram
enzyme variant Q192: 2.46% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 9.05% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
gamma-butyrolactone + H2O
gamma-hydroxybutyrate
show the reaction diagram
gamma-caprolactone + H2O
4-hydroxyhexanoic acid
show the reaction diagram
-
-
-
-
?
gamma-caprolactone + H2O
4-hydroxypentanoate
show the reaction diagram
-
-
-
-
?
gamma-caprolactone + H2O
gamma-hydroxycaproate
show the reaction diagram
gamma-decanolactone + H2O
4-hydroxydecanoic acid
show the reaction diagram
gamma-decanolactone + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-dodecalactone + H2O
4-hydroxydodecanoate
show the reaction diagram
-
-
-
-
?
gamma-dodecanoic acid lactone + H2O
4-hydroxydodecanoic acid
show the reaction diagram
-
-
-
-
?
gamma-dodecanoic lactone + H2O
4-hydroxydodecanoic acid
show the reaction diagram
-
-
-
?
gamma-dodecanolactone + H2O
4-hydroxydodecanoate
show the reaction diagram
-
-
-
?
gamma-heptalactone + H2O
4-hydroxyheptanoate
show the reaction diagram
-
-
-
-
?
gamma-heptalactone + H2O
4-hydroxyheptanoic acid
show the reaction diagram
-
-
-
?
gamma-heptalactone + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-heptanolactone + H2O
4-hydroxyheptanoate
show the reaction diagram
-
-
-
?
gamma-heptanolide + H2O
4-hydroxyheptanoate
show the reaction diagram
-
-
-
?
gamma-heptanolide + H2O
4-hydroxyheptanoic acid
show the reaction diagram
-
-
-
-
?
gamma-heptanolide + H2O
?
show the reaction diagram
-
-
-
?
gamma-hexalactone + H2O
4-hydroxyhexanoic acid
show the reaction diagram
-
-
-
?
gamma-hexalactone + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-nonalactone + H2O
4-hydroxynonanoate
show the reaction diagram
gamma-nonalactone + H2O
4-hydroxynonanoic acid
show the reaction diagram
gamma-nonanoic acid lactone + H2O
4-hydroxynonanoic acid
show the reaction diagram
-
-
-
-
?
gamma-octalactone + H2O
4-hydroxyoctanoate
show the reaction diagram
gamma-octalactone + H2O
4-hydroxyoctanoic acid
show the reaction diagram
-
-
-
?
gamma-octalactone + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-phenyl-gamma-butyrolactone + H2O
4-hydroxy-4-phenyl-butanoic acid
show the reaction diagram
-
-
-
?
gamma-phenyl-gamma-butyrolactone + H2O
4-hydroxy-4-phenylbutanoic acid
show the reaction diagram
-
-
-
?
gamma-undecalactone + H2O
4-hydroxyundecanoate
show the reaction diagram
-
-
-
-
?
gamma-undecanoic acid lactone + H2O
4-hydroxyundecanoic acid
show the reaction diagram
-
-
-
-
?
gamma-undecanoic lactone + H2O
4-hydroxyundecanoic acid
show the reaction diagram
-
-
-
?
gamma-undecanolactone + H2O
4-hydroxyundecanoate
show the reaction diagram
-
-
-
?
gamma-undecanolactone + H2O
4-hydroxyundecanoic acid
show the reaction diagram
-
-
-
?
gamma-undecanolactone + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-valerolactone + H2O
4-hydroxypentanoic acid
show the reaction diagram
gamma-valerolactone + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-valerolactone + H2O
gamma-hydroxyvalerate
show the reaction diagram
homocysteine thiolactone + H2O
?
show the reaction diagram
-
-
-
-
?
homocysteinthiolactone + H2O
(2S)-2-aminobutanethioic S-acid
show the reaction diagram
-
-
-
-
?
homogenestic acid lactone + H2O
?
show the reaction diagram
-
-
-
-
?
homogentisic acid lactone + H2O
homogentisic acid
show the reaction diagram
L-pantoyl lactone + H2O
L-pantoic acid
show the reaction diagram
lovastatin + H2O
?
show the reaction diagram
methyl 2-chloroacetate + H2O
?
show the reaction diagram
11.6% activity compared to DL-pantoyl lactone
-
-
?
methyl 2-chloropropanoate + H2O
?
show the reaction diagram
2.3% activity compared to DL-pantoyl lactone
-
-
?
methyl lactate + H2O
?
show the reaction diagram
less than 1% activity compared to DL-pantoyl lactone
-
-
?
methyl mandelate + H2O
?
show the reaction diagram
less than 1% activity compared to DL-pantoyl lactone
-
-
?
mevastatin + H2O
?
show the reaction diagram
activity with enzyme variant Q192 and R192
-
-
?
N-(2-oxotetrahydrofuran-3-yl)nonanamide + H2O
3-hydroxy-2-(nonanoylamino)propanoate
show the reaction diagram
-
-
-
-
?
N-(2-oxotetrahydrofuran-3-yl)pentanamide + H2O
3-hydroxy-2-(pentanoylamino)propanoate
show the reaction diagram
-
-
-
-
?
N-(2-oxotetrahydrofuran-3-yl)pentanamide + H2O
3-hydroxy-2-(pentanoylamino)propanoic acid
show the reaction diagram
-
-
-
?
N-(3-oxobutanoyl)-DL-homoserine lactone + H2O
N-(3-oxobutanoyl)-DL-homoserine
show the reaction diagram
-
-
-
?
N-(3-oxodecanoyl)-DL-homoserine lactone + H2O
N-(3-oxodecanoyl)-DL-homoserine
show the reaction diagram
-
-
-
?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
show the reaction diagram
N-(3-oxododecanoyl)-DL-homoserine lactone + H2O
N-(3-oxododecanoyl)-DL-homoserine
show the reaction diagram
-
-
-
?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
N-(3-oxododecanoyl)-L-homoserine
show the reaction diagram
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
show the reaction diagram
N-(3-oxohexanoyl)homoserine lactone + H2O
N-(3-oxohexanoyl)homoserine
show the reaction diagram
-
-
-
?
N-(3-oxooctanoyl)-DL-homoserine lactone + H2O
N-(3-oxooctanoyl)-DL-homoserine
show the reaction diagram
-
-
-
?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
show the reaction diagram
N-(3-oxooctanoyl)homoserine lactone + H2O
N-(3-oxooctanoyl)homoserine
show the reaction diagram
-
-
-
?
N-butanoyl-DL-homoserine lactone + H2O
N-butanoyl-L-homoserine
show the reaction diagram
-
-
-
?
N-butanoylhomoserine lactone + H2O
N-butanoylhomoserine
show the reaction diagram
-
-
-
?
N-dodecanoyl-DL-homoserine lactone + H2O
N-dodecanoyl-DL-homoserine
show the reaction diagram
-
-
-
?
N-dodecanoylhomoserine lactone + H2O
N-dodecanoylhomoserine
show the reaction diagram
-
-
-
?
N-hexanoyl-(S)-homoserine lactone
N-hexanoyl-(S)-homoserine
show the reaction diagram
N-hexanoylhomoserine lactone + H2O
N-hexanoylhomoserine
show the reaction diagram
-
-
-
?
N-octanoyl-DL-homoserine lactone + H2O
octanoyl-homoserine
show the reaction diagram
-
-
-
?
N-octanoylhomoserine lactone + H2O
N-octanoylhomoserine
show the reaction diagram
-
-
-
?
N-oxododecanoyl-DL-homoserine lactone + H2O
?
show the reaction diagram
-
-
-
-
?
nonanoic-1,4-lactone + H2O
4-hydroxynonanoate
show the reaction diagram
-
-
-
?
nonanoic-1,5-lactone + H2O
5-hydroxynonanoate
show the reaction diagram
-
-
-
?
nonanoic-delta-lactone + H2O
nonanoate
show the reaction diagram
-
-
-
?
nonanoic-gamma-lactone + H2O
nonanoate
show the reaction diagram
-
-
-
?
octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
show the reaction diagram
-
-
-
-
?
pantoyl lactone + H2O
2,4-dihydroxy-3,3-dimethylbutanoic acid
show the reaction diagram
-
-
-
-
?
paraoxon + H2O
?
show the reaction diagram
propylene carbonate + H2O
?
show the reaction diagram
enzyme variant Q192: 5.02% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 8.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
simvastatin + H2O
?
show the reaction diagram
activity with enzyme variant Q192 and R192
-
-
?
spironolactone + H2O
?
show the reaction diagram
thiobutyl butyrolactone + H2O
?
show the reaction diagram
-
-
-
-
?
undecano-gamma-lactone + H2O
4-hydroxyundecanoic acid
show the reaction diagram
enzyme variant Q192: 11.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 12.7% of the activity with phenyl acetate (cf. EC 3.1.1.2)
-
-
?
undecanoic-1,4-lactone + H2O
4-hydroxyundecanoate
show the reaction diagram
-
-
-
?
undecanoic-1,5-lactone + H2O
5-hydroxyundecanoate
show the reaction diagram
-
-
-
?
undecanoic-delta-lactone + H2O
undecanoate
show the reaction diagram
-
-
-
?
undecanoic-gamma-lactone + H2O
undecanoate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
homocysteine thiolactone + H2O
?
show the reaction diagram
-
-
-
-
?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
show the reaction diagram
Q97VT7
the best, possibly natural, substrate of wild-type enzyme
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+)
Mn2+
-
the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+)
Ni2+
-
the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+)
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-hydroxyquinoline
-
-
8-hydroxyquinoline
-
at 2 mM, 50% residual activity
Ag+
33.2% residual activity at 1 mM
Al3+
22.4% residual activity at 1 mM
AlCl3
-
at 2 mM, 66% residual activity
ascorbate
-
ascorbate/Cu2+ system is the most potent in inactivating the lactonase activity (by 90%). Cu2+ remarkably enhances the inactivation in the presence of ascorbate. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 95% inactivation. Ascorbate/Cu2+-mediated inactivation of lactonase activity is prevented by catalase (90%), oleic acid (73% at 0.01 mM) and dioleoylphosphatidylcholine (68% at 0.1 mM). Ascorbate/Fe2+ (0.5 mM/0.002 mM) system shows 20% inactivation
Co2+
10.7% residual activity at 1 mM
Cu+
13.6% residual activity at 1 mM
delta-undecanolactone
contrary to the wild-type enzyme and mutant enzyme W263F, the mutant enzymes W263M, W263L, W263I, W263V and W263T exhibit a substrate inhibition
eserine
-
weak inhibition
ethanol
-
6%, 85% inhibition
ethyl beta-hydroxybutyrate
-
weak inhibition
Fe3+
3.3% residual activity at 1 mM
FeCl2
-
at 2 mM, 69% residual activity
FeCl3
-
at 2 mM, 43% residual activity
Hg2+
2.1% residual activity at 1 mM
high density lipoprotein
-
incubation of serum or high density lipoprotein from healthy subjects with very low density lipoprotein significantly decreases serum paraoxonase 1 lactonase or arylesterase activities by up to 11% or 24%, and HDL-associated paraoxonase 1 lactonase or arylesterase activities by up to 32% or 46%, respectively
-
Li+
24.7% residual activity at 1 mM
Mn2+
22.7% residual activity at 1 mM
NaN3
-
at 2 mM, 84% residual activity
Neostigmine
-
-
Ni2+
55.4% residual activity at 1 mM
nitrilotriacetic acid
-
-
Pb2+
13.9% residual activity at 1 mM
PbCl2
-
at 2 mM, 62% residual activity
PMSF
-
1 mM
SnCl2
-
at 2 mM, 62% residual activity
sodium hypochlorite
-
at 1 mM and 10 mM causes approximately 18 and 34% decrease in lactonase activity, respectively
very low density lipoprotein
-
inhibits recombinant paraoxonase 1 lactonase or arylesterase activities by up to 20% or 42%, respectively
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
apolipoprotein apoA-I
-
only apolipoprotein apoA-I is capable of stimulating the lactonase activity, while apoA-IV and apoA-II have almost no effect
-
dilauroyl phosphatidylcholine
stimulates activity of enzyme variants Q192 and R192
phosphate
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.55
2-coumaranone
-
pH 7.4, 30C
290.2
2-hydroxybutyrolactone
recombinant enzyme, at pH 6.7-6.9, 30C
2.56
3,4-dihydrocoumarin
-
pH 7.4, 30C
4.5
3-hydroxyoctanoate
-
-
0.231
3-oxo-decanoyl-L-homoserine lactone
-
pH 8.3, 40C
0.186
3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 40C
0.27 - 1.83
5-(thiobutyl)butyrolactone
0.93
5-butyl-4-methyldihydro-2(3H)-furanone
-
pH 8.3, temperature not specified in the publication
0.836
7-oxo-N-(2-oxotetrahydrofuran-3-yl)nonanamide
pH 8.3, 25C
0.213
9-oxo-N-(2-oxotetrahydrofuran-3-yl)undecanamide
pH 8.3, 25C
0.013 - 0.02
coumaranone
0.839
delta-dodecalactone
-
pH 8.3, 40C
1.678
delta-dodecanolactone
pH 8.3, 25C, wild-type enzyme
0.359 - 1.22
delta-nonalactone
0.105
delta-undecalactone
-
pH 8.3, 40C
0.057 - 0.219
delta-undecanolactone
0.4 - 8.5
delta-valerolactone
0.013 - 1.376
dihydrocoumarin
121
DL-pantoyllactone
recombinant enzyme, at pH 6.7-6.9, 30C
0.242
dodecanoic-1,4-lactone
pH 8.3, 25C
0.185
dodecanoic-1,5-lactone
pH 8.3, 25C
0.12
dodecanoic-delta-lactone
pH 8.3, 25C, wild-type enzyme
0.48
dodecanoic-gamma-lactone
pH 8.3, 25C, wild-type enzyme
0.234 - 1.031
epsilon-caprolactone
0.55
gamma-(R)-caprolactone
-
pH 8.3, temperature not specified in the publication
4.56
gamma-(R)-valerolactone
-
pH 8.3, temperature not specified in the publication
0.75
gamma-(S)-caprolactone
-
pH 8.3, temperature not specified in the publication
1.95
gamma-(S)-valerolactone
-
pH 8.3, temperature not specified in the publication
0.16 - 21
gamma-butyrolactone
0.9 - 3.8
Gamma-caprolactone
0.0021
gamma-dodecalactone
-
pH 8.3, 40C
0.22
gamma-dodecanoic acid lactone
-
pH 8.0
0.3 - 0.7
gamma-dodecanoic lactone
1.22
gamma-dodecanolactone
pH 8.3, 25C, wild-type enzyme
0.872
gamma-heptalactone
-
pH 8.3, 40C
0.166
gamma-heptanolactone
pH 8.3, 25C, wild-type enzyme
0.13 - 0.58
gamma-heptanolide
140
gamma-hydroxybutyrate
-
-
5.7
Gamma-hydroxycaproate
-
-
9 - 20
Gamma-hydroxyvalerate
0.047 - 2.943
gamma-nonalactone
0.39
gamma-nonanoic acid lactone
-
pH 8.0
1.3 - 3.1
Gamma-octalactone
2.8 - 3.5
gamma-thiobutyrolactone
0.12
gamma-undecalactone
-
pH 8.3, 40C
0.6
gamma-undecanoic acid lactone
-
pH 8.0
0.6
gamma-undecanoiclactone
-
recombinant enzyme
0.013 - 2.099
gamma-undecanolactone
3.3 - 4
gamma-valerolactone
21.2 - 23.5
homocysteine thiolactone
0.45 - 0.52
Homogentisic acid lactone
3.59
L-pantoyl lactone
-
pH 7.4, 30C
0.178
N-(2-oxotetrahydrofuran-3-yl)pentanamide
pH 8.3, 25C
0.1
N-(3-oxodecanoyl)-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
0.143 - 1.605
N-(3-oxodecanoyl)-L-homoserine lactone
0.44
N-(3-oxododecanoyl)-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
0.0178 - 0.456
N-(3-oxododecanoyl)-L-homoserine lactone
0.558
N-(3-oxohexanoyl)-L-homoserine lactone
pH 8.3, 25C, wild-type enzyme
0.592
N-(3-oxohexanoyl)homoserine lactone
pH 8.3, 25C, wild-type enzyme
0.042 - 1.2
N-(3-oxooctanoyl)-DL-homoserine lactone
0.123
N-(3-oxooctanoyl)-L-homoserine lactone
pH 8.3, 25C, wild-type enzyme
0.256
N-(3-oxooctanoyl)homoserine lactone
pH 8.3, 25C, wild-type enzyme
1.3
N-dodecanoyl-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
0.345
N-dodecanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
0.459
N-hexanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
0.68
N-octanoyl-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
0.145
N-octanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
0.109
nonanoic-1,4-lactone
pH 8.3, 25C
0.348
nonanoic-1,5-lactone
pH 8.3, 25C
0.06
nonanoic-delta-lactone
25C, pH not specified in the publication, wild-type enzyme
0.015 - 0.061
nonanoic-gamma-lactone
0.262
octanoyl-L-homoserine lactone
-
pH 8.3, 40C
4.3
pantoyl lactone
-
pH 8.0
0.7 - 24.2
Paraoxon
0.83
phenyl acetate
-
pH 8.0, 37C
0.578
undecanoic-1,4-lactone
pH 8.3, 25C
0.168
undecanoic-1,5-lactone
pH 8.3, 25C
0.042 - 0.121
undecanoic-delta-lactone
0.39
undecanoic-gamma-lactone
pH 8.3, 25C, wild-type enzyme
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.47
3-oxo-decanoyl-L-homoserine lactone
-
pH 8.3, 40C
0.35
3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 40C
39
4-methoxy phenyl acetate
-
pH 8.0
1.74 - 116
5-(thiobutyl)butyrolactone
4.2
5-butyl-4-methyldihydro-2(3H)-furanone
-
pH 8.3, temperature not specified in the publication
0.89
7-oxo-N-(2-oxotetrahydrofuran-3-yl)nonanamide
pH 8.3, 25C
1.03
9-oxo-N-(2-oxotetrahydrofuran-3-yl)undecanamide
pH 8.3, 25C
28.08
delta-dodecalactone
-
pH 8.3, 40C
15.32 - 88.91
delta-nonalactone
60.78
delta-undecalactone
-
pH 8.3, 40C
7.38 - 93.3
delta-undecanolactone
0.43 - 632
delta-valerolactone
7.32 - 152
dihydrocoumarin
0.53
dodecanoic-1,4-lactone
pH 8.3, 25C
3.34
dodecanoic-1,5-lactone
pH 8.3, 25C
11.09
dodecanoic-delta-lactone
pH 8.3, 25C, wild-type enzyme
1.5
dodecanoic-gamma-lactone
pH 8.3, 25C, wild-type enzyme
4.45 - 15.04
epsilon-caprolactone
3.04
gamma-(R)-caprolactone
-
pH 8.3, temperature not specified in the publication
6.23
gamma-(R)-valerolactone
-
pH 8.3, temperature not specified in the publication
1.89
gamma-(S)-caprolactone
-
pH 8.3, temperature not specified in the publication
2.68
gamma-(S)-valerolactone
-
pH 8.3, temperature not specified in the publication
2.79 - 111
gamma-butyrolactone
2.27 - 112.3
Gamma-caprolactone
0.16
gamma-dodecalactone
-
pH 8.3, 40C
26.4
gamma-dodecanoic acid lactone
-
pH 8.0
34 - 101
gamma-dodecanoic lactone
2.72
gamma-dodecanolactone
pH 8.3, 25C, wild-type enzyme
27.25
gamma-heptalactone
-
pH 8.3, 40C
2.92
gamma-heptanolactone
pH 8.3, 25C, wild-type enzyme
5.9 - 34
gamma-heptanolide
0.38 - 44.49
gamma-nonalactone
31
gamma-nonanoic acid lactone
-
pH 8.0
8.86
gamma-undecalactone
-
pH 8.3, 40C
62
gamma-undecanoic acid lactone
-
pH 8.0
62
gamma-undecanoiclactone
-
recombinant enzyme
1.94 - 5.64
gamma-undecanolactone
0.94
N-(2-oxotetrahydrofuran-3-yl)pentanamide
pH 8.3, 25C
10.65
N-(3-oxodecanoyl)-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
0.107 - 4.52
N-(3-oxodecanoyl)-L-homoserine lactone
0.39
N-(3-oxododecanoyl)-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
0.41 - 6.44
N-(3-oxododecanoyl)-L-homoserine lactone
0.083
N-(3-oxohexanoyl)-L-homoserine lactone
pH 8.3, 25C, wild-type enzyme
0.041
N-(3-oxohexanoyl)homoserine lactone
pH 8.3, 25C, wild-type enzyme
0.85 - 4.1
N-(3-oxooctanoyl)-DL-homoserine lactone
0.54
N-(3-oxooctanoyl)-L-homoserine lactone
pH 8.3, 25C, wild-type enzyme
0.42
N-(3-oxooctanoyl)homoserine lactone
pH 8.3, 25C, wild-type enzyme
1.9
N-dodecanoyl-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
1.7
N-dodecanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
0.36
N-hexanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
0.71
N-octanoyl-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
1
N-octanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
2.64
nonanoic-1,4-lactone
pH 8.3, 25C
4.55
nonanoic-1,5-lactone
pH 8.3, 25C
51.7
nonanoic-delta-lactone
25C, pH not specified in the publication, wild-type enzyme
1.9 - 3.1
nonanoic-gamma-lactone
0.59
octanoyl-L-homoserine lactone
-
pH 8.3, 40C
11.9
pantoyl lactone
-
pH 8.0
6.82 - 12.6
Paraoxon
0.34
undecanoic-1,4-lactone
pH 8.3, 25C
1.05
undecanoic-1,5-lactone
pH 8.3, 25C
12.8 - 17.65
undecanoic-delta-lactone
2.15
undecanoic-gamma-lactone
pH 8.3, 25C, wild-type enzyme
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.3 - 131.6
2-coumaranone
2.05
3-oxo-decanoyl-L-homoserine lactone
-
pH 8.3, 40C
1.88
3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 40C
4.54
5-butyl-4-methyldihydro-2(3H)-furanone
-
pH 8.3, temperature not specified in the publication
1.07
7-oxo-N-(2-oxotetrahydrofuran-3-yl)nonanamide
pH 8.3, 25C
4.88
9-oxo-N-(2-oxotetrahydrofuran-3-yl)undecanamide
pH 8.3, 25C
0.0335
delta-dodecalactone
-
pH 8.3, 40C
7.54
delta-dodecanolactone
pH 8.3, 25C, wild-type enzyme
0.073 - 42.7
delta-nonalactone
0.058
delta-undecalactone
-
pH 8.3, 40C
78.6 - 792
delta-undecanolactone
0.22 - 19.8
delta-valerolactone
5.32
dihydrocoumarin
pH 8.3, 25C, wild-type enzyme
2.21
dodecanoic-1,4-lactone
pH 8.3, 25C
18.1
dodecanoic-1,5-lactone
pH 8.3, 25C
89.5
dodecanoic-delta-lactone
pH 8.3, 25C, wild-type enzyme
3.14
dodecanoic-gamma-lactone
pH 8.3, 25C, wild-type enzyme
14.6 - 19.8
epsilon-caprolactone
5.56
gamma-(R)-caprolactone
-
pH 8.3, temperature not specified in the publication
1.33
gamma-(R)-valerolactone
-
pH 8.3, temperature not specified in the publication
2.53
gamma-(S)-caprolactone
-
pH 8.3, temperature not specified in the publication
1.38
gamma-(S)-valerolactone
-
pH 8.3, temperature not specified in the publication
0.24 - 36.4
gamma-butyrolactone
0.03
Gamma-caprolactone
-
pH 8.3, 40C
0.078
gamma-dodecalactone
-
pH 8.3, 40C
2.23
gamma-dodecanolactone
pH 8.3, 25C, wild-type enzyme
0.031
gamma-heptalactone
-
pH 8.3, 40C
17.6
gamma-heptanolactone
pH 8.3, 25C, wild-type enzyme
26.4 - 46.1
gamma-heptanolide
0.948 - 1.88
gamma-nonalactone
0.074
gamma-undecalactone
-
pH 8.3, 40C
2.36 - 349
gamma-undecanolactone
1.11 - 21.2
homocysteine thiolactone
5.28
N-(2-oxotetrahydrofuran-3-yl)pentanamide
pH 8.3, 25C
96.3
N-(3-oxodecanoyl)-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
0.106 - 31.6
N-(3-oxodecanoyl)-L-homoserine lactone
0.9
N-(3-oxododecanoyl)-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
2.2 - 121
N-(3-oxododecanoyl)-L-homoserine lactone
0.15
N-(3-oxohexanoyl)-L-homoserine lactone
pH 8.3, 25C, wild-type enzyme
0.069
N-(3-oxohexanoyl)homoserine lactone
pH 8.3, 25C, wild-type enzyme
0.76 - 97
N-(3-oxooctanoyl)-DL-homoserine lactone
4.39
N-(3-oxooctanoyl)-L-homoserine lactone
pH 8.3, 25C, wild-type enzyme
1.63
N-(3-oxooctanoyl)homoserine lactone
pH 8.3, 25C, wild-type enzyme
0.004
N-butanoyl-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
0.012
N-butanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
1.46
N-dodecanoyl-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
4.9
N-dodecanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
0.78
N-hexanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
1.05
N-octanoyl-DL-homoserine lactone
25C, pH not specified in the publication, wild-type enzyme
6.9
N-octanoylhomoserine lactone
pH 8.3, 25C, wild-type enzyme
24.4
nonanoic-1,4-lactone
pH 8.3, 25C
13.1
nonanoic-1,5-lactone
pH 8.3, 25C
828
nonanoic-delta-lactone
25C, pH not specified in the publication, wild-type enzyme
31.3 - 204
nonanoic-gamma-lactone
2.23
octanoyl-L-homoserine lactone
-
pH 8.3, 40C
0.519 - 12.1
Paraoxon
0.59
undecanoic-1,4-lactone
pH 8.3, 25C
6.22
undecanoic-1,5-lactone
pH 8.3, 25C
106 - 335
undecanoic-delta-lactone
5.49
undecanoic-gamma-lactone
pH 8.3, 25C, wild-type enzyme
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.789 - 7.4
delta-undecanolactone
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0035
pH 8.0, 25C, substrate: DL-tetradecanoyl-homoserine lactone, purified recombinant enzyme
0.0036
pH 8.0, 25C, substrate: DL-heptanoyl-homoserine lactone, purified recombinant enzyme
0.0049
pH 8.0, 25C, substrate: DL-heptanoyl-homoserine lactone, purified recombinant enzyme
0.011
pH 8.0, 25C, substrate: spironolactone, purified recombinant enzyme
0.013
pH 8.0, 25C, substrate: canrenone, purified recombinant enzyme
0.0152
-
substrate gamma-butyrolactone
0.0167
pH 8.0, 25C, substrate: DL-dodecanoyl-homoserine lactone, purified recombinant enzyme
0.0255
pH 8.0, 25C, substrate: DL-tetradecanoyl-homoserine lactone, purified recombinant enzyme
0.0268
pH 8.0, 25C, substrate: DL-3-oxo-hexanoyl-homoserine lactone, purified recombinant enzyme
0.0311
pH 8.0, 25C, substrate: DL-heptanoyl-homoserine lactone, purified recombinant enzyme
0.0334
pH 8.0, 25C, substrate: DL-3-oxo-hexanoyl-homoserine lactone, purified recombinant enzyme
0.0977
pH 8.0, 25C, substrate: DL-dodecanoyl-homoserine lactone, purified recombinant enzyme
0.266
pH 8.0, 25C, substrate: lovastatin, purified recombinant enzyme
0.4239
pH 8.0, 25C, substrate: DL-tetradecanoyl-homoserine lactone, purified recombinant enzyme
0.4588
pH 8.0, 25C, substrate: DL-dodecanoyl-homoserine lactone, purified recombinant enzyme
0.68
pH 8.0, 25C, substrate: gamma-phenyl-gamma-butyrolactone, purified recombinant enzyme
0.81
pH 8.0, 25C, substrate: gamma-butyrolactone, purified recombinant enzyme
1.83
pH 8.0, 25C, substrate: 5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 1,5-lactone, purified recombinant enzyme
3.1
pH 8.0, 25C, substrate: dihydrocoumarin, purified recombinant enzyme
6.2
pH 8.0, 25C, substrate: gamma-valerolactone, purified recombinant enzyme
10.9
pH 8.0, 25C, substrate: 2-coumaranone, purified recombinant enzyme
11.1
pH 8.0, 25C, substrate: delta-nonalactone, purified recombinant enzyme
11.4
pH 8.0, 25C, substrate: gamma-phenyl-gamma-butyrolactone, purified recombinant enzyme
11.7
pH 8.0, 25C, substrate: delta-hexalactone, purified recombinant enzyme
14.5
pH 8.0, 25C, substrate: delta-valerolactone, purified recombinant enzyme
20.7
pH 8.0, 25C, substrate: alpha-angelicalactone, purified recombinant enzyme
22.7
pH 8.0, 25C, substrate: delta-tetradecanolactone, purified recombinant enzyme
23.9
pH 8.0, 25C, substrate: gamma-hexalactone, purified recombinant enzyme
25.6
pH 8.0, 25C, substrate: gamma-octalactone, purified recombinant enzyme
27.5
pH 8.0, 25C, substrate: 5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 1,5-lactone, purified recombinant enzyme
27.7
pH 8.0, 25C, substrate: gamma-heptalactone, purified recombinant enzyme
30.9
pH 8.0, 25C, substrate: gamma-nonalactone, purified recombinant enzyme
32.1
pH 8.0, 25C, substrate: gamma-butyrolactone, purified recombinant enzyme
40.7
pH 8.0, 25C, substrate: 2-coumaranone, purified recombinant enzyme
44.3
pH 8.0, 25C, substrate: delta-decanolactone, purified recombinant enzyme
45
pH 8.0, 25C, substrate: gamma-valerolactone, purified recombinant enzyme
45.6
pH 8.0, 25C, substrate: gamma-decanolactone, purified recombinant enzyme
51.7
pH 8.0, 25C, substrate: gamma-hexalactone, purified recombinant enzyme
57.2
pH 8.0, 25C, substrate: gamma-heptalactone, purified recombinant enzyme
63
pH 8.0, 25C, substrate: gamma-phenyl-gamma-butyrolactone, purified recombinant enzyme
69.2
pH 8.0, 25C, substrate: gamma-octalactone, purified recombinant enzyme
71.4
pH 8.0, 25C, substrate: gamma-undecanolactone, purified recombinant enzyme
72
pH 8.0, 25C, substrate: delta-hexalactone, purified recombinant enzyme
75.4
pH 8.0, 25C, substrate: 5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 1,5-lactone, purified recombinant enzyme
84.4
pH 8.0, 25C, substrate: delta-undecanolactone, purified recombinant enzyme
126.1
pH 8.0, 25C, substrate: dihydrocoumarin, purified recombinant enzyme
127.6
pH 8.0, 25C, substrate: gamma-undecanolactone, purified recombinant enzyme
129.9
pH 8.0, 25C, substrate: dihydrocoumarin, purified recombinant enzyme
135.7
pH 8.0, 25C, substrate: 2-coumaranone, purified recombinant enzyme
144.7
pH 8.0, 25C, substrate: gamma-nonalactone, purified recombinant enzyme
150
pH 8.0, 25C, substrate: delta-nonalactone, purified recombinant enzyme
154
pH 8.0, 25C, substrate: delta-tetradecanolactone, purified recombinant enzyme
173.8
pH 8.0, 25C, substrate: gamma-decanolactone, purified recombinant enzyme
183
pH 8.0, 25C, substrate: alpha-angelicalactone, purified recombinant enzyme
251
pH 8.0, 25C, substrate: delta-decanolactone, purified recombinant enzyme
287
pH 8.0, 25C, substrate: delta-undecanolactone, purified recombinant enzyme
329.5
pH 8.0, 25C, substrate: homogentisic acid lactone, purified recombinant enzyme
341.2
recombinant enzyme after 14.4fold purification, using DL-pantoyl lactone as substrate, at pH 6.7-6.9, 30C
549
-
substrate: 5-thiobutyl-butyro-1,4-lactone, pH 8.5, 70C, Mn2+-containing enzyme
671
pH 8.0, 25C, substrate: delta-valerolactone, purified recombinant enzyme
additional information
-
new enzymatic tests are developed that detect total PON1 levels, irrespective of high-density lipoprotein status and R/Q polymorphism, as well as the degree of catalytic stimulation and increased stability that follows tight binding of PON1 to HDLapoA-I. The tests are based on measuring total PON1 levels with a fluorogenic phosphotriester, measuring the lipolactonase activity with a chromogenic lactone, and assaying the chelator-mediated inactivation rate of the enzyme. The latter two are affected by tight binding of high-density lipoprotein and thereby derive the levels of the serum PON1-HDL complex. The new tests are demonstrated with a group of healthy individuals and show that the levels of PON1-HDL vary by a factor of 12
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 7.2
-
-
7.5
-
measured for gamma-butyrolactone hydrolysis
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
no activity below
5 - 10
-
pH 5.0: about 55% of maximal activity, pH 10.0: about 35% of maximal activity
6 - 9.6
pH 6.0: about 70% of maximal activity, pH 9.6: about 55% of maximal activity, hydrolysis of dihydrocoumarin
7 - 9
-
pH 7.0: about 30% of maximal activity, pH 9.0: about 80% of maximal activity, substrate: methyl paraoxon
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
measured for gamma-butyrolactone hydrolysis
80
-
substrate: methyl paraoxon
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 37
70 - 90
-
70C: about 60% of maximal activity, 90C: about 50% of maximal activity, substrate: methyl paraoxon
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
-
determined by two-dimensional electrophoresis
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycoplasma synoviae (strain 53)
Mycoplasma synoviae (strain 53)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17500
recombinant enzyme, nondenaturing PAGE
26500
-
2 * 26500, SDS-PAGE
35000
-
and 70000, SDS-PAGe and mass spectrometry
35548
-
and dimer, 1 * 35000, SDS-PAGE, 1 * 35548, mass spectrometry; and monomer, 2 * 35000, SDS-PAGE, 2 * 35548, mass spectrometry
36000
-
2 * 36000, SDS-PAGE
39000
2 * 39000, recombinant enzyme
42000
3 * 42000, recombinant enzyme, SDS-PAGE
43000
x * 43000, SDS-PAGE
45000
-
x * 45000, SDS-PAGE
50000
x * 50000, recombinant protein, SDS-PAGE
62000
-
gel filtration
68000
-
TSK-gel column
70000
-
and 35000, SDS-PAGe and mass spectrometry
80000
dynamic light scattering
82000
-
gel filtration
91900
serum enzyme, nondenaturing PAGE
94900
recombinant enzyme, nondenaturing PAGE
95600
recombinant enzyme, nondenaturing PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
monomer
trimer
3 * 42000, recombinant enzyme, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, in order to understand the structural consequences of variations at position 263, the crystal structures of the mutant enzymes (W263F, W263M, W263L, W263I, W263V, W263T) is solved
phosphate ion is bound to the catalytic calcium in the structure of crystallized recombinant G2E6
-
protein at pH 6.5 and in complex with 2-hydroxyquinoline. The models suggest that promiscuity is driven by coincidental overlaps between the reactive intermediate for the native lactonase reaction and the ground and/or intermediate states of the promiscuous reactions. This overlap is also enabled by different active-site conformations: the lactonase activity utilizes one active-site conformation whereas the promiscuous phosphotriesterase activity utilizes another
-
sitting drop vapor diffusion method at 20C, X-ray structures of the enzyme bound to a fatty acid and to its substrate acyl-homoserine lactone
-
to 2.4 A resolution, space group P64, with unit-cell parameters a=b=174.06, c = 61.32 A. The asymmetric unit contains one homodimer
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 9
-
lactonase activity is quite stable
663985
5 - 10
almost 100% activity is maintained during the incubation period of 180 min
649767
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 55
180 min, quite stable
50
-
stable below
60
90 min, 50% loss of activity
84
Tm-value of mutant enzyme W263L
92
Tm-value of mutant enzyme W263L
94
Tm-value, mutant enzyme Y34Q
95
half-life: 3.6 min
96
Tm-value, mutant enzyme E14K
98
Tm-value, mutant enzyme E14K/Y34Q
102
Tm-value, wild-type enzyme
additional information
mutant enzymes (W263F, W263M, W263L, W263I, W263V, W263T) exhibit a lower Tm-value compared to the wild-type enzyme