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Literature summary for 3.1.1.25 extracted from
- Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicus (2012), PLoS One, 7, e47028.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sulfolobus islandicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E14K | Tm-value for the mutant enzyme is 6°C lower than wild-type Tm-value. The efficiency of methyl-paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity | Sulfolobus islandicus |
| E14K/Y34Q | Tm-value for the mutant enzyme is 4°C lower than wild-type Tm-value. The efficiency of methyl-paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity | Sulfolobus islandicus |
| Y34Q | Tm-value for the mutant enzyme is 8°C lower than wild-type Tm-value. The efficiency of methyl-paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity | Sulfolobus islandicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus |  |
| 0.025 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 0.042 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 0.042 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.043 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 0.047 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 0.06 | - |
nonanoic-delta-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.061 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 0.1 | - |
N-(3-oxodecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.12 | - |
dodecanoic-delta-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 0.121 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 0.13 | - |
gamma-heptanolide | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 0.16 | - |
gamma-butyrolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 0.37 | - |
epsilon-caprolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 0.39 | - |
undecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 0.44 | - |
N-(3-oxododecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.48 | - |
dodecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 0.68 | - |
N-octanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.917 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 1 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 1.2 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 1.3 | - |
N-dodecanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 1.9 | - |
delta-valerolactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
dynamic light scattering | Sulfolobus islandicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus islandicus | C4KKZ9 | - |
- |
| Sulfolobus islandicus M.16.4 | C4KKZ9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus islandicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| delta-valerolactone + H2O | - |
Sulfolobus islandicus | ? | - |
? | |
| delta-valerolactone + H2O | - |
Sulfolobus islandicus M.16.4 | ? | - |
? | |
| dodecanoic-delta-lactone + H2O | - |
Sulfolobus islandicus | 5-hydroxydodecanoate | - |
? | |
| dodecanoic-delta-lactone + H2O | - |
Sulfolobus islandicus M.16.4 | 5-hydroxydodecanoate | - |
? | |
| dodecanoic-gamma-lactone + H2O | - |
Sulfolobus islandicus | dodecanoate | - |
? | |
| epsilon-caprolactone + H2O | - |
Sulfolobus islandicus | ? | - |
? | |
| epsilon-caprolactone + H2O | - |
Sulfolobus islandicus M.16.4 | ? | - |
? | |
| gamma-butyrolactone + H2O | - |
Sulfolobus islandicus | 4-hydroxybutanoate | - |
? | |
| gamma-heptanolide + H2O | - |
Sulfolobus islandicus | ? | - |
? | |
| N-(3-oxobutanoyl)-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-(3-oxobutanoyl)-DL-homoserine | - |
? | |
| N-(3-oxodecanoyl)-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-(3-oxodecanoyl)-DL-homoserine | - |
? | |
| N-(3-oxododecanoyl)-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-(3-oxododecanoyl)-DL-homoserine | - |
? | |
| N-(3-oxooctanoyl)-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-(3-oxooctanoyl)-DL-homoserine | - |
? | |
| N-butanoyl-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-butanoyl-L-homoserine | - |
? | |
| N-dodecanoyl-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | N-dodecanoyl-DL-homoserine | - |
? | |
| N-octanoyl-DL-homoserine lactone + H2O | - |
Sulfolobus islandicus | octanoyl-homoserine | - |
? | |
| nonanoic-delta-lactone + H2O | - |
Sulfolobus islandicus | 5-hydroxynonanoate | - |
? | |
| nonanoic-gamma-lactone + H2O | - |
Sulfolobus islandicus | 4-hydroxynonanoate | - |
? | |
| undecanoic-delta-lactone + H2O | - |
Sulfolobus islandicus | 5-hydroxyundecanoate | - |
? | |
| undecanoic-gamma-lactone + H2O | - |
Sulfolobus islandicus | 4-hydroxyundecanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | at 25°C, dynamic light scattering | Sulfolobus islandicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SisLac | the enzyme also shows activity of aryldialkylphosphatase (EC 3.1.8.1) and arylesterase (EC 3.1.1.2) | Sulfolobus islandicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life: 84 min | Sulfolobus islandicus |
| 90 | - |
half-life: 8.5 min | Sulfolobus islandicus |
| 94 | - |
Tm-value, mutant enzyme Y34Q | Sulfolobus islandicus |
| 95 | - |
half-life: 3.6 min | Sulfolobus islandicus |
| 96 | - |
Tm-value, mutant enzyme E14K | Sulfolobus islandicus |
| 98 | - |
Tm-value, mutant enzyme E14K/Y34Q | Sulfolobus islandicus |
| 102 | - |
Tm-value, wild-type enzyme | Sulfolobus islandicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
N-(3-oxododecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.43 | - |
delta-valerolactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.71 | - |
N-octanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.85 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 0.92 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 0.97 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 1.5 | - |
dodecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 1.9 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 1.9 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 1.9 | - |
N-dodecanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 2 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 2.15 | - |
undecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 3.1 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 4.1 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 5.75 | - |
gamma-butyrolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 5.9 | - |
gamma-heptanolide | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 7.27 | - |
epsilon-caprolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 10.65 | - |
N-(3-oxodecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 11.09 | - |
dodecanoic-delta-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 12.8 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 12.9 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 14.1 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 17.65 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 51.7 | - |
nonanoic-delta-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
N-butanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.22 | - |
delta-valerolactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.76 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 0.9 | - |
N-(3-oxododecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 0.93 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 0.95 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 1.05 | - |
N-octanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 1.46 | - |
N-dodecanoyl-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 3.14 | - |
dodecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 5.49 | - |
undecanoic-gamma-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 19.8 | - |
epsilon-caprolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 31.3 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 36.4 | - |
gamma-butyrolactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 42.3 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 46.1 | - |
gamma-heptanolide | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 75.2 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 89.5 | - |
dodecanoic-delta-lactone | pH 8.3, 25°C, wild-type enzyme | Sulfolobus islandicus | |
| 96.3 | - |
N-(3-oxodecanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 97 | - |
N-(3-oxooctanoyl)-DL-homoserine lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 106 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme Y34Q | Sulfolobus islandicus | |
| 204 | - |
nonanoic-gamma-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
| 300 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K/Y34Q | Sulfolobus islandicus | |
| 335 | - |
undecanoic-delta-lactone | 25°C, pH not specified in the publication, mutant enzyme E14K | Sulfolobus islandicus | |
| 828 | - |
nonanoic-delta-lactone | 25°C, pH not specified in the publication, wild-type enzyme | Sulfolobus islandicus | |
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