Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.25 extracted from

  • Draganov, D.I.; Teiber, J.F.; Speelman, A.; Osawa, Y.; Sunahara, R.; La Du, B.N.
    Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities (2005), J. Lipid Res., 46, 1239-1247.
    View publication on PubMed
Search for more literature for 3.1.1.25

Cloned(Commentary)

Cloned (Comment) Organism
the recombinant enzyme is expressed in Trichoplusia ni High Five insect cells Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17500
-
 recombinant enzyme, nondenaturing PAGE Homo sapiens
39000
-
 2 * 39000, recombinant enzyme Homo sapiens
40000
-
 2 * 40000, SDS-PAGE Homo sapiens
42000
-
 3 * 42000, recombinant enzyme, SDS-PAGE Homo sapiens
91900
-
 serum enzyme, nondenaturing PAGE Homo sapiens
94900
-
 recombinant enzyme, nondenaturing PAGE Homo sapiens
95600
-
 recombinant enzyme, nondenaturing PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27169
-
-
Homo sapiens Q15165
-
-
Homo sapiens Q15166
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0035
-
 pH 8.0, 25°C, substrate: DL-tetradecanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.0036
-
 pH 8.0, 25°C, substrate: DL-heptanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.0049
-
 pH 8.0, 25°C, substrate: DL-heptanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.011
-
 pH 8.0, 25°C, substrate: spironolactone, purified recombinant enzyme Homo sapiens
0.013
-
 pH 8.0, 25°C, substrate: canrenone, purified recombinant enzyme Homo sapiens
0.0167
-
 pH 8.0, 25°C, substrate: DL-dodecanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.0255
-
 pH 8.0, 25°C, substrate: DL-tetradecanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.0268
-
 pH 8.0, 25°C, substrate: DL-3-oxo-hexanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.0311
-
 pH 8.0, 25°C, substrate: DL-heptanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.0334
-
 pH 8.0, 25°C, substrate: DL-3-oxo-hexanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.0977
-
 pH 8.0, 25°C, substrate: DL-dodecanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.266
-
 pH 8.0, 25°C, substrate: lovastatin, purified recombinant enzyme Homo sapiens
0.4239
-
 pH 8.0, 25°C, substrate: DL-tetradecanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.4588
-
 pH 8.0, 25°C, substrate: DL-dodecanoyl-homoserine lactone, purified recombinant enzyme Homo sapiens
0.68
-
 pH 8.0, 25°C, substrate: gamma-phenyl-gamma-butyrolactone, purified recombinant enzyme Homo sapiens
0.81
-
 pH 8.0, 25°C, substrate: gamma-butyrolactone, purified recombinant enzyme Homo sapiens
1.83
-
 pH 8.0, 25°C, substrate: 5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 1,5-lactone, purified recombinant enzyme Homo sapiens
3.1
-
 pH 8.0, 25°C, substrate: dihydrocoumarin, purified recombinant enzyme Homo sapiens
6.2
-
 pH 8.0, 25°C, substrate: gamma-valerolactone, purified recombinant enzyme Homo sapiens
10.9
-
 pH 8.0, 25°C, substrate: 2-coumaranone, purified recombinant enzyme Homo sapiens
11.1
-
 pH 8.0, 25°C, substrate: delta-nonalactone, purified recombinant enzyme Homo sapiens
11.4
-
 pH 8.0, 25°C, substrate: gamma-phenyl-gamma-butyrolactone, purified recombinant enzyme Homo sapiens
11.7
-
 pH 8.0, 25°C, substrate: delta-hexalactone, purified recombinant enzyme Homo sapiens
14.5
-
 pH 8.0, 25°C, substrate: delta-valerolactone, purified recombinant enzyme Homo sapiens
20.7
-
 pH 8.0, 25°C, substrate: alpha-angelicalactone, purified recombinant enzyme Homo sapiens
22.7
-
 pH 8.0, 25°C, substrate: delta-tetradecanolactone, purified recombinant enzyme Homo sapiens
23.9
-
 pH 8.0, 25°C, substrate: gamma-hexalactone, purified recombinant enzyme Homo sapiens
25.6
-
 pH 8.0, 25°C, substrate: gamma-octalactone, purified recombinant enzyme Homo sapiens
27.5
-
 pH 8.0, 25°C, substrate: 5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 1,5-lactone, purified recombinant enzyme Homo sapiens
27.7
-
 pH 8.0, 25°C, substrate: gamma-heptalactone, purified recombinant enzyme Homo sapiens
30.9
-
 pH 8.0, 25°C, substrate: gamma-nonalactone, purified recombinant enzyme Homo sapiens
32.1
-
 pH 8.0, 25°C, substrate: gamma-butyrolactone, purified recombinant enzyme Homo sapiens
40.7
-
 pH 8.0, 25°C, substrate: 2-coumaranone, purified recombinant enzyme Homo sapiens
44.3
-
 pH 8.0, 25°C, substrate: delta-decanolactone, purified recombinant enzyme Homo sapiens
45
-
 pH 8.0, 25°C, substrate: gamma-valerolactone, purified recombinant enzyme Homo sapiens
45.6
-
 pH 8.0, 25°C, substrate: gamma-decanolactone, purified recombinant enzyme Homo sapiens
51.7
-
 pH 8.0, 25°C, substrate: gamma-hexalactone, purified recombinant enzyme Homo sapiens
57.2
-
 pH 8.0, 25°C, substrate: gamma-heptalactone, purified recombinant enzyme Homo sapiens
63
-
 pH 8.0, 25°C, substrate: gamma-phenyl-gamma-butyrolactone, purified recombinant enzyme Homo sapiens
69.2
-
 pH 8.0, 25°C, substrate: gamma-octalactone, purified recombinant enzyme Homo sapiens
71.4
-
 pH 8.0, 25°C, substrate: gamma-undecanolactone, purified recombinant enzyme Homo sapiens
72
-
 pH 8.0, 25°C, substrate: delta-hexalactone, purified recombinant enzyme Homo sapiens
75.4
-
 pH 8.0, 25°C, substrate: 5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 1,5-lactone, purified recombinant enzyme Homo sapiens
84.4
-
 pH 8.0, 25°C, substrate: delta-undecanolactone, purified recombinant enzyme Homo sapiens
126.1
-
 pH 8.0, 25°C, substrate: dihydrocoumarin, purified recombinant enzyme Homo sapiens
127.6
-
 pH 8.0, 25°C, substrate: gamma-undecanolactone, purified recombinant enzyme Homo sapiens
129.9
-
 pH 8.0, 25°C, substrate: dihydrocoumarin, purified recombinant enzyme Homo sapiens
135.7
-
 pH 8.0, 25°C, substrate: 2-coumaranone, purified recombinant enzyme Homo sapiens
144.7
-
 pH 8.0, 25°C, substrate: gamma-nonalactone, purified recombinant enzyme Homo sapiens
150
-
 pH 8.0, 25°C, substrate: delta-nonalactone, purified recombinant enzyme Homo sapiens
154
-
 pH 8.0, 25°C, substrate: delta-tetradecanolactone, purified recombinant enzyme Homo sapiens
173.8
-
 pH 8.0, 25°C, substrate: gamma-decanolactone, purified recombinant enzyme Homo sapiens
183
-
 pH 8.0, 25°C, substrate: alpha-angelicalactone, purified recombinant enzyme Homo sapiens
251
-
 pH 8.0, 25°C, substrate: delta-decanolactone, purified recombinant enzyme Homo sapiens
287
-
 pH 8.0, 25°C, substrate: delta-undecanolactone, purified recombinant enzyme Homo sapiens
329.5
-
 pH 8.0, 25°C, substrate: homogentisic acid lactone, purified recombinant enzyme Homo sapiens
671
-
 pH 8.0, 25°C, substrate: delta-valerolactone, purified recombinant enzyme Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 6 months, less than 15% loss of activity Homo sapiens
4°C, purified recombinant enzyme preparations typically lose about 20% of the arylesterase activity within the first month after purification, compared with less than 10% loss for the enzyme purified from serum. There is no further significant loss of the enzyme activity afterward, suggesting the existence of two pools in the purified enzyme, a labile one and a stable one Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-coumaranone + H2O
-
 Homo sapiens (2-hydroxyphenyl)acetic acid
-
 ?
5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoic acid 1,5-lactone + H2O
-
 Homo sapiens 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoic acid
-
 ?
5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 1,5-lactone + H2O
-
 Homo sapiens 5-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid
-
 ?
alpha-angelicalactone + H2O
-
 Homo sapiens 4-hydroxy-pent-3-enoic acid
-
 ?
alpha-angelicalactone + H2O
-
 Homo sapiens 4-hydroxypent-3-enoic acid
-
 ?
canrenone + H2O not hydrolyzed by PON1 (P27169 (UniProt)) and PON2 (Q15165 (UniProt)) Homo sapiens ?
-
 ?
delta-decanolactone + H2O
-
 Homo sapiens 5-hydroxydecanoic acid
-
 ?
delta-hexalactone + H2O
-
 Homo sapiens 5-hydroxyhexanoic acid
-
 ?
delta-nonalactone + H2O
-
 Homo sapiens 5-hydroxynonanoic acid
-
 ?
delta-tetradecanolactone + H2O
-
 Homo sapiens 5-hydroxytetradecanoic acid
-
 ?
delta-undecanolactone + H2O
-
 Homo sapiens 5-hydroxyundecanoic acid
-
 ?
delta-valerolactone + H2O
-
 Homo sapiens 5-hydroxypentanoic acid
-
 ?
dihydrocoumarin + H2O
-
 Homo sapiens 3-(2-hydroxyphenyl)propanoic acid
-
 ?
DL-3-oxo-hexanoyl-homoserine lactone + H2O
-
 Homo sapiens DL-3-oxo-hexanoyl-homoserine
-
 ?
DL-3-oxo-hexanoylhomoserine lactone + H2O
-
 Homo sapiens DL-3-oxo-hexanoylhomoserine
-
 ?
DL-dodecanoyl-homoserine lactone + H2O
-
 Homo sapiens DL-dodecanoyl-homoserine
-
 ?
DL-dodecanoylhomoserine lactone + H2O
-
 Homo sapiens DL-dodecanoylhomoserine
-
 ?
DL-heptanoyl-homoserine lactone + H2O
-
 Homo sapiens DL-heptanoyl-homoserine
-
 ?
DL-heptanoylhomoserine lactone + H2O
-
 Homo sapiens DL-heptanoylhomoserine
-
 ?
DL-tetradecanoyl-homoserine lactone + H2O
-
 Homo sapiens DL-tetradecanoyl-homoserine
-
 ?
DL-tetradecanoylhomoserine lactone + H2O
-
 Homo sapiens DL-tetradecanoyl-homoserine
-
 ?
DL-tetradecanoylhomoserine lactone + H2O
-
 Homo sapiens DL-tetradecanoylhomoserine
-
 ?
gamma-butyrolactone + H2O
-
 Homo sapiens 4-hydroxy-butyric acid
-
 ?
gamma-butyrolactone + H2O
-
 Homo sapiens 4-hydroxybutyric acid
-
 ?
gamma-decanolactone + H2O
-
 Homo sapiens 4-hydroxydecanoic acid
-
 ?
gamma-heptalactone + H2O
-
 Homo sapiens 4-hydroxyheptanoic acid
-
 ?
gamma-hexalactone + H2O
-
 Homo sapiens 4-hydroxyhexanoic acid
-
 ?
gamma-nonalactone + H2O
-
 Homo sapiens 4-hydroxynonanoic acid
-
 ?
gamma-octalactone + H2O
-
 Homo sapiens 4-hydroxyoctanoic acid
-
 ?
gamma-phenyl-gamma-butyrolactone + H2O
-
 Homo sapiens 4-hydroxy-4-phenyl-butanoic acid
-
 ?
gamma-phenyl-gamma-butyrolactone + H2O
-
 Homo sapiens 4-hydroxy-4-phenylbutanoic acid
-
 ?
gamma-undecanolactone + H2O
-
 Homo sapiens 4-hydroxyundecanoic acid
-
 ?
gamma-valerolactone + H2O
-
 Homo sapiens 4-hydroxypentanoic acid
-
 ?
homogentisic acid lactone + H2O
-
 Homo sapiens homogentisic acid
-
 ?
lovastatin + H2O not hydrolyzed by PON1 (P27169 (UniProt)) and PON2 (Q15165 (UniProt)) Homo sapiens ?
-
 ?
additional information no activity with homogentisic acid lactone and DL-3-oxo-hexanoyl-homoserine lactone Homo sapiens ?
-
 ?
additional information no activity with lovastatin, spironolactone or canrenone Homo sapiens ?
-
 ?
additional information no activity with lovastatin, spironolactone, canrenone, homogentisic acid lactone, gamma-butyrolactone, gamma-valerolactone, gamma-hexalactone, gamma-heptalactone, gamma-octalactone, gamma-nonalactone, gamma-decanolactone, gamma-undecanolactone, alpha-angelicalactone, delta-valerolactone, delta-hexalactone, delta-nonalactone, delta-decanolactone, delta-undecanolactone, delta-tetradecanolactone Homo sapiens ?
-
 ?
spironolactone + H2O not hydrolyzed by PON1 (P27169 (UniProt)) and PON2 (Q15165 (UniProt)) Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Homo sapiens
dimer 2 * 39000, recombinant enzyme Homo sapiens
trimer 3 * 42000, recombinant enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens