Literature summary for 3.1.1.2 extracted from

Otto, T.C.; Kasten, S.A.; Kovaleva, E.; Liu, Z.; Buchman, G.; Tolosa, M.; Davis, D.; Smith, J.R.; Balcerzak, R.; Lenz, D.E.; Cerasoli, D.M.
Purification and characterization of functional human paraoxonase-1 expressed in Trichoplusia ni larvae (2010), Chem. Biol. Interact., 187, 388-392.

Search for more literature for 3.1.1.2

Application

Application	Comment	Organism
analysis	the enzyme has the potential to be used as a catalytic bioscavenger of nerve agents. Insect production of the enzyme may provide a source for both in vitro enzymatic and crystallographic studies and in vivo stability and anti-nerve agent efficacy testing	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed from Trichoplusia ni larvae	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.5	-	phenyl acetate	in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41000	-	x * 41000, glycosylated enzyme, SDS-PAGE or mass spectrometry, x * 42000, glycosylated enzyme, SDS-PAGE or mass spectrometry, x * 44000, glycosylated enzyme, SDS-PAGE or mass spectrometry	Homo sapiens
42000	-	x * 41000, glycosylated enzyme, SDS-PAGE or mass spectrometry, x * 42000, glycosylated enzyme, SDS-PAGE or mass spectrometry, x * 44000, glycosylated enzyme, SDS-PAGE or mass spectrometry	Homo sapiens
44000	-	x * 41000, glycosylated enzyme, SDS-PAGE or mass spectrometry, x * 42000, glycosylated enzyme, SDS-PAGE or mass spectrometry, x * 44000, glycosylated enzyme, SDS-PAGE or mass spectrometry	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by immobilized metal-ion affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Storage Stability

Storage Stability	Organism
4°C, 2 weeks	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phenyl acetate + H2O	-	Homo sapiens	phenol + acetate	-	?

Subunits

Subunits	Comment	Organism
?	x * 41000, glycosylated enzyme, SDS-PAGE or mass spectrometry, x * 42000, glycosylated enzyme, SDS-PAGE or mass spectrometry, x * 44000, glycosylated enzyme, SDS-PAGE or mass spectrometry	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
850	-	phenyl acetate	in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	caterpillar of the Trichoplusia ni moth can be used as an expression system to produce large quantities of functional recombinant enzyme	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1670	-	phenyl acetate	in 50 mM Tris-HCl buffer, 10 mM CaCl2, pH 7.4	Homo sapiens

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Release 2023.1 (January 2023)