Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | optimal concentration is 3.5% at 35°C | Lactobacillus helveticus |
Cloned (Comment) | Organism |
---|---|
gene estA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha as C-terminally HIs-tagged protein | Lactobacillus helveticus |
Protein Variants | Comment | Organism |
---|---|---|
C105A | site-directed mutagenesis, 55.9% of the wild-type activity with substrate 4-nitrophenyl butyrate | Lactobacillus helveticus |
H137A | site-directed mutagenesis, 17.4% of the wild-type activity with substrate 4-nitrophenyl butyrate | Lactobacillus helveticus |
H164A | site-directed mutagenesis, inactive mutant | Lactobacillus helveticus |
H56A | site-directed mutagenesis, 30.0% of the wild-type activity with substrate 4-nitrophenyl butyrate | Lactobacillus helveticus |
S10A | site-directed mutagenesis, inactive mutant | Lactobacillus helveticus |
S112A | site-directed mutagenesis, 99.5% of the wild-type activity with substrate 4-nitrophenyl butyrate | Lactobacillus helveticus |
S138G | site-directed mutagenesis, 99.2% of the wild-type activity with substrate 4-nitrophenyl butyrate | Lactobacillus helveticus |
S155A | site-directed mutagenesis, 99.0% of the wild-type activity with substrate 4-nitrophenyl butyrate | Lactobacillus helveticus |
S42A | site-directed mutagenesis, 33.2% of the wild-type activity with substrate 4-nitrophenyl butyrate | Lactobacillus helveticus |
S44A | site-directed mutagenesis, 33.7% of the wild-type activity with substrate 4-nitrophenyl butyrate | Lactobacillus helveticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | - |
Lactobacillus helveticus | |
additional information | no inhibition by EDTA and iodoacetic acid | Lactobacillus helveticus | |
pepstatin A | - |
Lactobacillus helveticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22600 | 25100 | gel filtration on Sephacryl 100 and 300 | Lactobacillus helveticus |
23400 | - |
1 * 23400, SDS-PAGE | Lactobacillus helveticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus helveticus | Q9LAH7 | enzyme EstA, gene estA | - |
Lactobacillus helveticus CNRZ32 | Q9LAH7 | enzyme EstA, gene estA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli, to homogeneity | Lactobacillus helveticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Lactobacillus helveticus |
486 | 519 | purified recombinant wild-type enzyme, substrate phenyl acetate | Lactobacillus helveticus |
623 | - |
purified recombinant wild-type enzyme, substrate phenyl acetate in presence of 3.5% NaCl | Lactobacillus helveticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-acetoxypyridine + H2O | - |
Lactobacillus helveticus | ? | - |
? | |
4-acetoxybenzaldehyde + H2O | - |
Lactobacillus helveticus | ? | - |
? | |
4-nitrophenyl acetate + H2O | - |
Lactobacillus helveticus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Lactobacillus helveticus CNRZ32 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Lactobacillus helveticus | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Lactobacillus helveticus | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caprylate + H2O | - |
Lactobacillus helveticus | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Lactobacillus helveticus | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Lactobacillus helveticus CNRZ32 | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl valerate + H2O | - |
Lactobacillus helveticus | 4-nitrophenol + valerate | - |
? | |
additional information | substrate specificity, no activity with 4-nitrophenyl caprate, 4-nitrophenyl laurate, 4-nitrophenyl phosphate, 4-nitrophenyl sulfate, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl phenylphosphonate, stearoyl-CoA, palmitoyl-CoA, methylacetylsalicylate, ethyl acetate, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, isoamyl acetate, and caproyl acetate | Lactobacillus helveticus | ? | - |
? | |
additional information | substrate specificity, no activity with 4-nitrophenyl caprate, 4-nitrophenyl laurate, 4-nitrophenyl phosphate, 4-nitrophenyl sulfate, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl phenylphosphonate, stearoyl-CoA, palmitoyl-CoA, methylacetylsalicylate, ethyl acetate, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, isoamyl acetate, and caproyl acetate | Lactobacillus helveticus CNRZ32 | ? | - |
? | |
phenyl acetate + H2O | best substrate | Lactobacillus helveticus | phenol + acetate | - |
? | |
phenyl acetate + H2O | best substrate | Lactobacillus helveticus CNRZ32 | phenol + acetate | - |
? | |
phenylthioacetate + H2O | - |
Lactobacillus helveticus | thiophenol + acetate | - |
? | |
phenylthioacetate + H2O | - |
Lactobacillus helveticus CNRZ32 | thiophenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 23400, SDS-PAGE | Lactobacillus helveticus |
Synonyms | Comment | Organism |
---|---|---|
ESt-A | - |
Lactobacillus helveticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Lactobacillus helveticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Lactobacillus helveticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lactobacillus helveticus | isoelectric focusing | - |
8 |