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Literature summary for 3.1.1.14 extracted from

  • Schenk, N.; Schelbert, S.; Kanwischer, M.; Goldschmidt, E.E.; Doermann, P.; Hoertensteiner, S.
    The chlorophyllases AtCLH1 and AtCLH2 are not essential for senescence-related chlorophyll breakdown in Arabidopsis thaliana (2007), FEBS Lett., 581, 5517-5525.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene chl1, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH1 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis Arabidopsis thaliana
gene chl2, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH2 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic vesicle cytoplasmic Arabidopsis thaliana 31410
-
additional information no localization in chloroplasts of isozyme AtCLH1 Arabidopsis thaliana
-
-
additional information no localization in chloroplasts of isozyme AtCLH2 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyll + H2O Arabidopsis thaliana isozyme AtCLH1 is not essential for chlorophyll breakdown during plant senescence, overview phytol + chlorophyllide
-
?
chlorophyll + H2O Arabidopsis thaliana isozyme AtCLH2 is not essential for chlorophyll breakdown during plant senescence, overview phytol + chlorophyllide
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O22527 isozyme AtCLH1; isozyme AtCLH1
-
Arabidopsis thaliana Q9M7I7 isozyme AtCLH2; isozyme AtCLH2
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf green, isozyme AtCLH1 expression analysis Arabidopsis thaliana
-
leaf green, isozyme AtCLH2 expression analysis Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll + H2O isozyme AtCLH1 is not essential for chlorophyll breakdown during plant senescence, overview Arabidopsis thaliana phytol + chlorophyllide
-
?
chlorophyll + H2O isozyme AtCLH2 is not essential for chlorophyll breakdown during plant senescence, overview Arabidopsis thaliana phytol + chlorophyllide
-
?
chlorophyll + H2O removal of the lipophilic phytol moiety of chlorophyll Arabidopsis thaliana phytol + chlorophyllide
-
?

Synonyms

Synonyms Comment Organism
AtCLH1
-
Arabidopsis thaliana
AtCLH2
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Arabidopsis thaliana