Cloned (Comment) | Organism |
---|---|
gene chl1, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH1 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis | Arabidopsis thaliana |
gene chl2, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH2 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic vesicle | cytoplasmic | Arabidopsis thaliana | 31410 | - |
additional information | no localization in chloroplasts of isozyme AtCLH1 | Arabidopsis thaliana | - |
- |
additional information | no localization in chloroplasts of isozyme AtCLH2 | Arabidopsis thaliana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | Arabidopsis thaliana | isozyme AtCLH1 is not essential for chlorophyll breakdown during plant senescence, overview | phytol + chlorophyllide | - |
? | |
chlorophyll + H2O | Arabidopsis thaliana | isozyme AtCLH2 is not essential for chlorophyll breakdown during plant senescence, overview | phytol + chlorophyllide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O22527 | isozyme AtCLH1; isozyme AtCLH1 | - |
Arabidopsis thaliana | Q9M7I7 | isozyme AtCLH2; isozyme AtCLH2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | green, isozyme AtCLH1 expression analysis | Arabidopsis thaliana | - |
leaf | green, isozyme AtCLH2 expression analysis | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | isozyme AtCLH1 is not essential for chlorophyll breakdown during plant senescence, overview | Arabidopsis thaliana | phytol + chlorophyllide | - |
? | |
chlorophyll + H2O | isozyme AtCLH2 is not essential for chlorophyll breakdown during plant senescence, overview | Arabidopsis thaliana | phytol + chlorophyllide | - |
? | |
chlorophyll + H2O | removal of the lipophilic phytol moiety of chlorophyll | Arabidopsis thaliana | phytol + chlorophyllide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtCLH1 | - |
Arabidopsis thaliana |
AtCLH2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |