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Literature summary for 3.1.1.14 extracted from

  • Arkus, K.A.; Cahoon, E.B.; Jez, J.M.
    Mechanistic analysis of wheat chlorophyllase (2005), Arch. Biochem. Biophys., 438, 146-155.
    View publication on PubMed

Application

Application Comment Organism
agriculture manipulation of chlorophyll degradation Triticum aestivum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
p-nitrophenyl palmitate final concentration of 10% v/v acetonitrile at 37°C Triticum aestivum
0.52
-
p-nitrophenyl decanoate final concentration of 10% v/v acetonitrile at 37°C Triticum aestivum
69
-
Chlorophyll at 25°C Triticum aestivum
570
-
p-nitrophenyl butyrate final concentration of 10% v/v acetonitrile at 25°C Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33880
-
calculation from sequence of cDNA Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyll + H2O Triticum aestivum
-
chlorophyllide + phytol
-
ir

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
wheat
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf etiolated seedling Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
21 chlorophyllide a at 25°C, 10 chlorophyllide b at 25°C Triticum aestivum

Storage Stability

Storage Stability Organism
-80°C, 25 mM Tris buffer, pH 8.0, 100 mM NaCl Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll + H2O
-
Triticum aestivum chlorophyllide + phytol
-
ir
p-nitrophenyl butyrate + H2O
-
Triticum aestivum p-nitrophenol + butyrate
-
?
p-nitrophenyl decanoate + H2O
-
Triticum aestivum p-nitrophenol + decanoate
-
?
p-nitrophenyl palmitate + H2O
-
Triticum aestivum p-nitrophenol + palmitate
-
?

Synonyms

Synonyms Comment Organism
chlorophyll-chlorophyllidohydrolase
-
Triticum aestivum
chlorophyllase
-
Triticum aestivum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 75
-
Triticum aestivum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
p-nitrophenyl palmitate
-
Triticum aestivum
0.52
-
p-nitrophenyl decanoate
-
Triticum aestivum
10.3
-
p-nitrophenyl butyrate
-
Triticum aestivum
566
-
Chlorophyll
-
Triticum aestivum

pI Value

Organism Comment pI Value Maximum pI Value
Triticum aestivum calculation from sequence of cDNA
-
5.7