Literature summary for 3.1.1.13 extracted from

Natarajan, R.; Ghosh, S.; McLean Grogan, W.
Catalytic properties of the purified rat hepatic cytosolic cholesteryl ester hydrolase (1996), Biochem. Biophys. Res. Commun., 225, 413-419.

Search for more literature for 3.1.1.13

Activating Compound

Activating Compound	Comment	Organism	Structure
sodium taurocholic acid	0.25 mM prevents aggregation of the enzyme into an inactive state, 3fold stimulation with more lipophilic substrates at 5 mM	Rattus sp.

General Stability

General Stability	Organism
mild digestion results in progressive and selective loss of activity	Rattus sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Rattus sp.
Hg2+	-	Rattus sp.
iodoacetamide	modest inactivation	Rattus sp.
N-ethylmaleimide	modest inactivation	Rattus sp.
p-mercuribenzoate	30% inhibition at 3 mM	Rattus sp.
Phenylmethylsulfonylfluoride	50% inhibition at 0.1 mM	Rattus sp.
sodium taurocholic acid	inhibition higher than 2.5 mM for cholesteryl oleate and triolein hydrolysis	Rattus sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus sp.	5829	-
microsome	-	Rattus sp.	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cholesteryl esters + H2O	Rattus sp.	-	cholesterol + fatty acid	-	r

Organism

Organism	UniProt	Comment	Textmining
Rattus sp.	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus sp.	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesteryl esters + H2O	-	Rattus sp.	cholesterol + fatty acid	-	r
cholesteryl linoleate + H2O	activity lower than with oleate esters	Rattus sp.	cholesterol + linoleic acid	-	r
cholesteryl oleate + H2O	-	Rattus sp.	cholesterol + oleic acid	-	r
p-nitrophenyl acetate + H2O	low activity	Rattus sp.	p-nitrophenol + acetic acid	-	r
p-nitrophenyl caprylate + H2O	activity increases with more lipophilic substrates	Rattus sp.	p-nitrophenol + caprylic acid	-	?
triolein + H2O	-	Rattus sp.	dioleoylglycerol + oleic acid	-	r

Synonyms

Synonyms	Comment	Organism
CEH	-	Rattus sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.1	-	Phenylmethylsulfonylfluoride	-	Rattus sp.

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Release 2023.1 (January 2023)