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Information on EC 3.1.1.13 - sterol esterase
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3.1.1.13 sterol esteraseIUBMB Comments
A group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts.
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Word Map
- 3.1.1.13
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cessation
- triglyceride
-
smoking
- infant
-
social
-
services
-
proliferate
-
physician
-
acyltransferase
- oleate
-
decision
- atherosclerosis
-
fire
-
discharge
- triacylglycerols
-
policy
-
esterify
-
awareness
-
foam
-
lipases
-
firing
-
esterification
-
australia
-
psychological
-
ethic
- hepatomegaly
-
practitioner
- taurocholate
-
advice
-
professionals
-
quit
-
financial
- nurses
-
legal
-
abstinence
-
acyl-coa:cholesterol
-
doctor
-
breastfeeding
-
unesterified
-
moral
-
childbearing
-
waking
-
a:cholesterol
- triolein
-
political
-
retirement
-
caregivers
-
breast-fed
-
hepatosplenomegaly
-
profession
- nutrition
- analysis
- paper production
- medicine
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
cease, lysosomal acid lipase, cholesterol ester hydrolase, cholesteryl ester hydrolase, pancreatic cholesterol esterase, bile salt-stimulated lipase, cholesteryl esterase, neutral cholesteryl ester hydrolase, bile salt-dependent lipase, kiaa1363, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acid cholesterol esterase
Acid cholesteryl ester hydrolase
-
-
-
-
acid lipase
acylcholesterol lipase
-
-
-
-
bile salt-activated lipase
bile salt-dependent cholesteryl ester hydrolase
bile salt-dependent lipase
BSDL
carboxylester lipase
CE hydrolase
CEase
CEH
ChE
cholesterase
-
-
-
-
cholesterol ester hydrolase
-
-
-
-
cholesterol ester synthetase
-
-
-
-
cholesterol esterase
cholesteryl ester hydrolase
cholesteryl ester synthetase
-
-
-
-
cholesteryl esterase
-
-
-
-
KIAA1263/AADACL1
KIAA1363
LAL
lysosomal acid lipase
NCEH
Nceh1
neutral cholesterol ester hydrolase
neutral cholesterol ester hydrolase 1
neutral cholesterol esterase
sterol ester hydrolase
-
-
-
-
Sterol esterase
sterol-ester acylhydrolase
-
-
-
-
triterpenol esterase
-
-
-
-
bile salt-dependent lipase
-
contains triacylglycerol hydrolase, sterol ester hydrolase, and wax ester hydrolase activities
bile salt-dependent lipase
-
contains triacylglycerol hydrolase, sterol ester hydrolase, and wax ester hydrolase activities
CEase
-
-
cholesterol esterase
-
-
-
-
cholesteryl ester hydrolase
-
-
-
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LAL
-
-
-
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Sterol esterase
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a steryl ester + H2O = a sterol + a fatty acid
a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, which may also bring about the esterification of sterols. Activated by bile salts; broad specificity
-
-
-
a steryl ester + H2O = a sterol + a fatty acid
a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts, the catalytic tiad is formed by Ser209, Glu341, and His449
-
a steryl ester + H2O = a sterol + a fatty acid
a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts, reaction mechanism, catalytic triad consists of Ser194, His435, and Asp320
-
a steryl ester + H2O = a sterol + a fatty acid
a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts
-
a steryl ester + H2O = a sterol + a fatty acid
a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts
-
a steryl ester + H2O = a sterol + a fatty acid
a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts
-
a steryl ester + H2O = a sterol + a fatty acid
a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts
-
a steryl ester + H2O = a sterol + a fatty acid
a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts
-
a steryl ester + H2O = a sterol + a fatty acid
a group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
esterification
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-
-
-
hydrolysis of carboxylic ester
hydrolysis of carboxylic ester
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-
-
-
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PATHWAY SOURCE
PATHWAYS
KEGG
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SYSTEMATIC NAME
IUBMB Comments
steryl-ester acylhydrolase
A group of enzymes of broad specificity, acting on esters of sterols and long-chain fatty acids, that may also bring about the esterification of sterols. Activated by bile salts.
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CAS REGISTRY NUMBER
COMMENTARY
9026-00-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-(diethylamino)-5,6,7,8-tetrahydro-4H-[1]benzothieno[2,3-d][1,3]oxazin-4-one + H2O
?
-
-
-
?
2-(diethylamino)-6,7-dihydro-4H,5H-cyclopenta[4,5]thieno[2,3-d][1,3]oxazin-4-one + H2O
?
-
-
-
?
2-(diethylamino)-6,7-dihydro-4H,5H-cyclopenta[4,5]thieno[2,3-d][1,3]thiazin-4-one + H2O
?
-
-
-
?
2-(dimethylamino)-1,5,6,7-tetrahydro-2H,4H-cyclopenta(4,5)thieno(2,3-d)(1,3)oxazin-4-one + H2O
?
-
-
-
-
?
4-methylumbelliferyl oleate + H2O
4-methylumbelliferone + oleic acid
-
-
-
?
cholesterol + oleate
cholesteryl oleate + H2O
-
100% esterification with 50 units of enzyme after 20 h. The enzyme is largely independent of water content in the synthesis of cholesteryl oleate, and it achieves near-complete esterification in the presence of an equimolar excess of oleate
-
-
?
cholesterol ester + H2O
cholesterol + fatty acid
-
hydrolysis level greatly exceeds the synthesis level, enzyme can simultaneously perform synthesis and hydrolysis reactions
-
r
cholesterol hexanoate + H2O
cholesterol + hexanoic acid
29% of the activity with cholesteryl linoleate
-
-
?
cholesterol palmitate + H2O
cholesterol + palmitic acid
5% of the activity with cholesteryl linoleate
-
-
?
p-nitrophenyl caprylate + H2O
p-nitrophenol + caprylic acid
-
activity increases with more lipophilic substrates
-
?
p-nitrophenyl laurate + H2O
p-nitrophenol + lauric acid
92% of the activity with cholesteryl linoleate
-
-
?
p-nitrophenyl propionate + H2O
p-nitrophenol + propionic acid
17% of the activity with cholesteryl linoleate
-
-
?
p-nitrophenyl stearate + H2O
p-nitrophenol + stearic acid
38% of the activity with cholesteryl linoleate
-
-
?
sitosterol oleate + H2O
sitosterol + oleate
-
about 60% activity compared to cholesteryl oleate
-
-
?
sitosterol palmitate + H2O
sitosterol + palmitate
-
about 30% activity compared to cholesteryl oleate
-
-
?
sitosterol stearate + H2O
sitosterol + stearate
-
about 30% activity compared to cholesteryl oleate
-
-
?
stigmastanol oleate + H2O
stigmastanol + oleate
-
about 65% activity compared to cholesteryl oleate
-
-
?
stigmastanol palmitate + H2O
stigmastanol + palmitate
-
about 30% activity compared to cholesteryl oleate
-
-
?
stigmastanol stearate + H2O
stigmastanol + stearate
-
about 20% activity compared to cholesteryl oleate
-
-
?
stigmasterol oleate + H2O
stigmasterol + oleate
-
about 35% activity compared to cholesteryl oleate
-
-
?
stigmasterol palmitate + H2O
stigmasterol + palmitate
-
about 15% activity compared to cholesteryl oleate
-
-
?
stigmasterol stearate + H2O
stigmasterol + stearate
-
about 15% activity compared to cholesteryl oleate
-
-
?
4-nitrophenyl laurate + H2O
4-nitrophenol + laurate
Q82JC7
-
-
-
?
4-nitrophenyl myristate + H2O
4-nitrophenol + myristate
Q82JC7
-
-
-
?
4-nitrophenyl palmitate + H2O
4-nitrophenol + palmitate
Q82JC7
-
-
-
?
4-nitrophenyl palmitate + H2O
4-nitrophenol + palmitate
Q82JC7
-
-
-
?
4-nitrophenyl palmitate + H2O
4-nitrophenol + palmitate
-
-
-
-
?
bis(2-ethylhexyl) phthalate + H2O
mono(2-ethylhexyl)phthalate + ?
-
complete hydrolysis within 15 min
-
-
?
bis(2-ethylhexyl) phthalate + H2O
mono(2-ethylhexyl)phthalate + ?
-
complete hydrolysis within 15 min
-
-
?
cholesterol acetate + H2O
cholesterol + acetic acid
3% of the activity with cholesteryl linoleate
-
-
?
cholesterol acetate + H2O
cholesterol + acetic acid
3% of the activity with cholesteryl linoleate
-
-
?
cholesterol butyrate + H2O
cholesterol + butyric acid
18% of the activity with cholesteryl linoleate
-
-
?
cholesterol butyrate + H2O
cholesterol + butyric acid
18% of the activity with cholesteryl linoleate
-
-
?
cholesterol decanoate + H2O
cholesterol + decanoic acid
34% of the activity with cholesteryl linoleate
-
-
?
cholesterol decanoate + H2O
cholesterol + decanoic acid
34% of the activity with cholesteryl linoleate
-
-
?
cholesterol oleate + H2O
cholesterol + oleic acid
53% of the activity with cholesteryl linoleate
-
-
?
cholesterol oleate + H2O
cholesterol + oleic acid
53% of the activity with cholesteryl linoleate
-
-
?
cholesteryl acetate + H2O
cholesterol + acetate
-
low activity, 6% of the activity with cholesteryl linoleate
-
?
cholesteryl acetate + H2O
cholesterol + acetate
Q82JC7
-
-
-
?
cholesteryl acetate + H2O
cholesterol + acetate
-
9% activity compared to linoleate
-
-
?
cholesteryl acetate + H2O
cholesterol + acetate
-
9% activity compared to linoleate
-
-
?
cholesteryl acetate + H2O
cholesterol + acetic acid
-
very slowly hydrolyzed by acid cholesterol esterase, only hydrolyzed by neutral cholestrol esterase at pH 8.0
-
?
cholesteryl butyrate + H2O
cholesterol + butanoic acid
-
very slowly hydrolyzed by acid cholesterol esterase, only hydrolyzed by neutral cholesterol esterase at pH 8.0
-
?
cholesteryl butyrate + H2O
cholesterol + butyrate
-
low activity, 8% of the activity with cholesteryl linoleate
-
?
cholesteryl butyrate + H2O
cholesterol + butyrate
-
121% activity compared to linoleate
-
-
?
cholesteryl caprate + H2O
cholesterol + caprate
-
40% of the activity with cholesteryl linoleate
-
?
cholesteryl caprate + H2O
cholesterol + caprate
-
21% activity compared to linoleate
-
-
?
cholesteryl caproate + H2O
cholesterol + caproate
-
22% of the activity with cholesteryl linoleate
-
?
cholesteryl caproate + H2O
cholesterol + caproate
-
37% activity compared to linoleate
-
-
?
cholesteryl caprylate + H2O
cholesterol + caprylate
-
62% of the activity with cholesteryl linoleate
-
?
cholesteryl caprylate + H2O
cholesterol + caprylate
-
79% activity compared to linoleate
-
-
?
cholesteryl erucate + H2O
cholesterol + erucic acid
-
-
13-docosenoic acid
?
cholesteryl erucate + H2O
cholesterol + erucic acid
-
-
13-docosenoic acid
?
cholesteryl esters + H2O
cholesterol + fatty acid
-
important rate-limiting step in steroidogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important rate-limiting step in steroidogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important rate-limiting step in steroidogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
shows also lipoamidase activity
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
acid cholesterol esterase hydrolized only long-chain cholesteryl esters, activity is low for substrate concentration below the critical micelle concentration
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
important rate-limiting step in steroidogenesis
-
?
cholesteryl esters + H2O
cholesterol + fatty acid
-
long-chain fatty acid cholesterol esters more easily synthesized, synthetic activity highest in chloroform, activity enhanced in degree of unsaturation of fatty acids
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
shows also lipoamidase activity
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in inhibition of spermatogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in inhibition of spermatogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
fatty acid binding site more hydrophobic than steroid binding site
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
hydrolytic activity toward liposomal and microcrystallic substrates
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
absoprtion of free cholesterol
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
fatty acid binding site more hydrophobic than steroid binding site
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
r
cholesteryl laurate + H2O
cholesterol + laurate
-
41% of the activity with cholesteryl linoleate
-
?
cholesteryl laurate + H2O
cholesterol + laurate
-
35% activity compared to linoleate
-
-
?
cholesteryl linoleate + H2O
cholesterol + linoleate
-
best substrate
-
-
?
cholesteryl linoleate + H2O
cholesterol + linoleate
-
best substrate
-
?
cholesteryl linoleate + H2O
cholesterol + linoleate
-
best substrate
-
?
cholesteryl linoleate + H2O
cholesterol + linoleate
Q82JC7
-
-
-
?
cholesteryl linoleate + H2O
cholesterol + linoleate
-
100% activity
-
-
?
cholesteryl linoleate + H2O
cholesterol + linoleic acid
-
most suitable substrate
-
?
cholesteryl linoleate + H2O
cholesterol + linoleic acid
-
synthesis of cholesterol linoleate from polyethylene glycol-modified enzyme only in water-immiscible solvents
-
r
cholesteryl linoleate + H2O
cholesterol + linoleic acid
-
activity lower than with oleate esters
-
r
cholesteryl myristate + H2O
cholesterol + myristate
-
58% of the activity with cholesteryl linoleate
-
?
cholesteryl myristate + H2O
cholesterol + myristate
-
42% activity compared to linoleate
-
-
?
cholesteryl nervonate + H2O
cholesterol + nervonic acid
-
especially for temperature-labile cholesteryl esterase
-
?
cholesteryl oleate
cholesterol + oleate
-
the cholesterol which is incorporated into brush border membrane is not esterified
-
?
cholesteryl oleate
cholesterol + oleate
-
the cholesterol which is incorporated into brush border membrane is not esterified
-
?
cholesteryl oleate + H2O
cholesterol + oleate
-
92% of the activity with cholesteryl linoleate
-
?
cholesteryl oleate + H2O
cholesterol + oleate
-
100% activity, best substrate
-
-
?
cholesteryl oleate + H2O
cholesterol + oleate
-
21% activity compared to linoleate
-
-
?
cholesteryl oleate + H2O
cholesterol + oleate
-
21% activity compared to linoleate
-
-
?
cholesteryl palmitate + H2O
cholesterol + palmitate
-
75% of the activity with cholesteryl linoleate
-
?
cholesteryl palmitate + H2O
cholesterol + palmitate
Q82JC7
-
-
-
?
cholesteryl palmitate + H2O
cholesterol + palmitate
-
about 50% activity compared to cholesteryl oleate
-
-
?
cholesteryl palmitate + H2O
cholesterol + palmitate
-
38% activity compared to linoleate
-
-
?
cholesteryl palmitate + H2O
cholesterol + palmitate
-
38% activity compared to linoleate
-
-
?
cholesteryl palmitate + H2O
cholesterol + palmitic acid
-
low activity
-
?
cholesteryl stearate + H2O
cholesterol + stearate
-
80% of the activity with cholesteryl linoleate
-
?
cholesteryl stearate + H2O
cholesterol + stearate
-
about 45% activity compared to cholesteryl oleate
-
-
?
cholesteryl stearate + H2O
cholesterol + stearate
-
18% activity compared to linoleate
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
5% of the activity with cholesteryl linoleate
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
5% of the activity with cholesteryl linoleate
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoic acid
53% of the activity with cholesteryl linoleate
-
-
?
p-nitrophenyl myristate + H2O
p-nitrophenol + myristic acid
100% of the activity with cholesteryl linoleate
-
-
?
p-nitrophenyl octanoate + H2O
p-nitrophenol + octanoic acid
79% of the activity with cholesteryl linoleate
-
-
?
p-nitrophenyl palmitate + H2O
p-nitrophenol + palmitic acid
68% of the activity with cholesteryl linoleate
-
-
?
triolein + H2O
diolein + oleate
-
positional specificity for sn-2-position, lower affinity for the sn-1- and sn-3-position
-
?
additional information
?
-
-
rank of the relative rates of hydrolysis by Acinetobacter sp. strain CHE4-1 enzyme are linoleate > oleate > acetate > palmitate > butyrate > laurate > caproate
-
-
-
additional information
?
-
-
rank of the relative rates of hydrolysis by Acinetobacter sp. strain CHE4-1 enzyme are linoleate > oleate > acetate > palmitate > butyrate > laurate > caproate
-
-
-
additional information
?
-
-
enzyme is involved in fatty acid catabolism
-
?
additional information
?
-
-
the enzyme accelerates the incorporation of cholesterol into human adenocarcinomal Caco-2 cells, which is not influenced by addition of exogenous oleic acid, the enzyme also significantly increases the incorporation of cholesterol into the intestinal brush border membranes of rat
-
?
additional information
?
-
-
enzyme catalyzes cleavage of fatty acid residues in both the sn-1 and sn-2 positions of phospholipids
-
-
-
additional information
?
-
-
CRL1 slowly hydrolyzes 6-chloro-2-pyrones
-
?
additional information
?
-
-
enzyme prefers substrates of long-chain fatty acids and unsaturated fatty acids
-
?
additional information
?
-
-
in aqueous dispersion, reaction proceeds slowly. Addition of surfactant Thesit accelerates initial reaction rate
-
-
-
additional information
?
-
-
the enzyme accelerates the incorporation of cholesterol into human adenocarcinomal Caco-2 cells, which is not influenced by addition of exogenous oleic acid
-
?
additional information
?
-
-
neutral lipid metabolism in macrophages contributes to organ inflammation and pathogenesis
-
-
-
additional information
?
-
-
neutral cholesterol ester hydrolase is not a target of protein kinase A
-
-
-
additional information
?
-
-
no activity with triacetin, enzyme activity increases with increasing chain lebgth and number of double bonds, no interfacial activation
-
?
additional information
?
-
-
the enzyme does not hydrolyze bis(2-ethylhexyl) phthalate, diethyl phthalate, di-n-propyl phthalate, di-n-butyl phthalate, di-n-pentyl phthalate, di-n-hexyl phthalate, n-butyl benzyl phthalate and dicyclohexyl phthalate
-
-
-
additional information
?
-
-
the enzyme within the pancreatic juice significantly increased the incorporation of cholesterol into the intestinal brush border membranes of rat
-
?
additional information
?
-
-
the enzyme is also active on fatty acid esters of methanol and 4-nitrophenol
-
-
-
additional information
?
-
-
the enzyme catalyzes reactions between cholesterol and methyl oleate/triolein. The degree of esterification of cholesterol with methyl oleate is 31% at the most after 19 h. The use of triolein as acyl donor results in lower esterification
-
-
-
additional information
?
-
-
the hydrolytic activity of the enzyme increases from cholesterol acetate to caproate, then decreases sharply from caproate to caprate, with a further gradual decrease through stearate. In esterification there is virtually no activity with short-chain free fatty acids from acetate through caproate, a marked increase with caprylate, and a constant level from caprylate through stearate
-
-
-
additional information
?
-
-
the enzyme catalyzes reactions between cholesterol and methyl oleate/triolein. The degree of esterification of cholesterol with methyl oleate is 31% at the most after 19 h. The use of triolein as acyl donor results in lower esterification
-
-
-
additional information
?
-
-
the hydrolytic activity of the enzyme increases from cholesterol acetate to caproate, then decreases sharply from caproate to caprate, with a further gradual decrease through stearate. In esterification there is virtually no activity with short-chain free fatty acids from acetate through caproate, a marked increase with caprylate, and a constant level from caprylate through stearate
-
-
-
additional information
?
-
-
the enzyme is also active on fatty acid esters of methanol and 4-nitrophenol
-
-
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cholesteryl esters + H2O
cholesterol + fatty acid
-
important rate-limiting step in steroidogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important rate-limiting step in steroidogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important rate-limiting step in steroidogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
shows also lipoamidase activity
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
acid cholesterol esterase hydrolized only long-chain cholesteryl esters, activity is low for substrate concentration below the critical micelle concentration
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
important rate-limiting step in steroidogenesis
-
?
cholesteryl esters + H2O
cholesterol + fatty acid
-
long-chain fatty acid cholesterol esters more easily synthesized, synthetic activity highest in chloroform, activity enhanced in degree of unsaturation of fatty acids
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
shows also lipoamidase activity
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in inhibition of spermatogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in inhibition of spermatogenesis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
fatty acid binding site more hydrophobic than steroid binding site
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
involvement in arteriosclerosis
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
hydrolytic activity toward liposomal and microcrystallic substrates
-
-
cholesteryl esters + H2O
cholesterol + fatty acid
-
absoprtion of free cholesterol
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
fatty acid binding site more hydrophobic than steroid binding site
-
r
cholesteryl esters + H2O
cholesterol + fatty acid
-
important role in fat digestion in infants
-
r
cholesteryl oleate
cholesterol + oleate
-
the cholesterol which is incorporated into brush border membrane is not esterified
-
?
cholesteryl oleate
cholesterol + oleate
-
the cholesterol which is incorporated into brush border membrane is not esterified
-
?
additional information
?
-
-
enzyme is involved in fatty acid catabolism
-
?
additional information
?
-
-
the enzyme accelerates the incorporation of cholesterol into human adenocarcinomal Caco-2 cells, which is not influenced by addition of exogenous oleic acid, the enzyme also significantly increases the incorporation of cholesterol into the intestinal brush border membranes of rat
-
?
additional information
?
-
-
the enzyme accelerates the incorporation of cholesterol into human adenocarcinomal Caco-2 cells, which is not influenced by addition of exogenous oleic acid
-
?
additional information
?
-
-
neutral lipid metabolism in macrophages contributes to organ inflammation and pathogenesis
-
-
-
additional information
?
-
-
the enzyme within the pancreatic juice significantly increased the incorporation of cholesterol into the intestinal brush border membranes of rat
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cAMP
-
activation only for neutral CEase; as cofactor for cAMP-dependent protein kinase
MgATP2-
-
activation only for neutral CEase; increasing activity with addition of cAMP-dependend protein kinase
MgATP2-
-
increasing activity with addition of cAMP-dependend protein kinase
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Cu2+
Mg2+
additional information
Mg2+
-
activation in a reaction mixture containing cAMP, protein kinase A and ATP
additional information
-
divalent metal ions do not influence the enzyme activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(R)-exo-2-norbornyl-N-n-butylcarbamate
-
the R-enantiomer is 6.8times more potent than the S-enantiomer
(S)-endo-2-norbornyl-N-n-butyl-carbamate
-
the S-enantiomer is 4.6times more potent than the R-enantiomer
2-(5,6,7,8)-tetrahydronaphthyl-N-hexyl carbamate
-
biphasic inhibition time-course
2-(diethylamino)-5,6,7,8-tetrahydro-4H-[1]benzothieno[2,3-d][1,3]oxazin-4-one
-
substrate inhibitor
2-(diethylamino)-5-methyl-5,6,7,8-tetrahydro-4H-(1)benzothieno(2,3-d)(1,3)oxazin-4-one
-
-
2-(diethylamino)-6,7,8,9-tetrahydro-4H,5H-cyclohepta(4,5)thieno(2,3-d)(1,3)oxazin-4-one
-
-
2-(diethylamino)-6,7-dihydro-4H,5H-cyclopenta(4,5)thieno(2,3-d)(1,3)oxazin-4-one
-
i.e. DO-3, significant inhibition of cleavage of cholesteryl esters in high density lipoproteins
2-(diethylamino)-6,7-dihydro-4H,5H-cyclopenta[4,5]thieno[2,3-d][1,3]oxazin-4-one
-
substrate inhibitor
2-(diethylamino)-6,7-dihydro-4H,5H-cyclopenta[4,5]thieno[2,3-d][1,3]thiazin-4-one
-
substrate inhibitor
2-(dimethylamino)-1,2,5,6,7,8-hexahydro-4H-(1)benzothieno(2,3-d)(1,3)oxazin-4-one
-
-
2-(dimethylamino)-1,5,6,7-tetrahydro-2H,4H-cyclopenta(4,5)thieno(2,3-d)(1,3)oxazin-4-one
-
-
2-(dimethylamino)-1,5,6,8-tetrahydro-2H,4H-thiopyrano(4',3':4,5)thieno(2,3-d)(1,3)oxazin-4-one
-
-
2-[3,4-bis[(diethoxyphosphinyl)oxy]phenyl]-4-oxo-4H-1-benzopyran-3,5,7-triyl phosphoric acid hexaethyl ester
-
-
2-[3,4-bis[(diethoxyphosphinyl)oxy]phenyl]-5-hydroxy-4-oxo-4H-1-benzopyran-3,7-diyl phosphoric acid tetraethyl ester
-
-
2-[3,4-bis[(diethoxyphosphoryl)oxy]phenyl]-4-oxo-4H-chromene-5,7-diyl tetraethyl bis(phosphate)
-
-
2-[3-[(diethoxyphosphinyl)oxy]-4-methoxy-phenyl]-4-oxo-4H-1-benzopyran-5,7-diyl phosphoric acid tetraethyl ester
-
-
2-[4-[(diethoxyphosphoryl)oxy]phenyl]-4-oxo-4H-chromene-5,7-diyl tetraethyl bis(phosphate)
-
-
2-[7-chloro-1-ethoxy-3-(4-ethylphenyl)-1-oxido-1H-2,1-benzoxaphosphinin-4-yl]-1-phenylethanone
-
-
2-[[4-(diethoxyphosphinyl)oxy]phenyl]-5-hydroxy-4-oxo-4H-1-benzopyran-7-yl phosphoric acid diethyl ester
-
-
3,3'-[4-([2-[(4-chlorophenyl)carbonyl]hydrazinyl]sulfonyl)-1,2-oxazole-3,5-diyl]bis(N-hydroxypropanamide)
-
-
3-[[4-(diethoxyphosphinyl)oxy]phenyl]-4-oxo-4H-1-benzopyran-5,7-diyl phosphoric acid tetraethyl ester
-
-
3-[[4-(diethoxyphosphinyl)oxy]phenyl]-4-oxo-4H-1-benzopyran-7-yl phosphoric acid diethyl ester
-
-
3-[[4-(diethoxyphosphinyl)oxy]phenyl]-5-hydroxy-4-oxo-4H-1-benzopyran-7-yl phosphoric acid diethyl ester
-
-
4-bromo-7-chloro-1-ethoxy-3-(4-methoxyphenyl)-1H-2,1-benzoxaphosphinine 1-oxide
-
-
4-nitrophenyl-N-allyl carbamate
-
mechanism of inhibition by these pseudo-substrate inhibitors, via a 2-step reaction including the formation of a tetrahedral enzyme-inhibitor adduct and the formation of the carbamyl-enzyme, inhibition kinetics
4-nitrophenyl-N-butyl carbamate
-
mechanism of inhibition by these pseudo-substrate inhibitors, via a 2-step reaction including the formation of a tetrahedral enzyme-inhibitor adduct and the formation of the carbamyl-enzyme, inhibition kinetics
4-nitrophenyl-N-chloroethyl carbamate
-
mechanism of inhibition by these pseudo-substrate inhibitors, via a 2-step reaction including the formation of a tetrahedral enzyme-inhibitor adduct and the formation of the carbamyl-enzyme, inhibition kinetics
4-nitrophenyl-N-hexyl carbamate
-
mechanism of inhibition by these pseudo-substrate inhibitors, via a 2-step reaction including the formation of a tetrahedral enzyme-inhibitor adduct and the formation of the carbamyl-enzyme, inhibition kinetics
4-nitrophenyl-N-methylphenyl carbamate
-
mechanism of inhibition by these pseudo-substrate inhibitors, via a 2-step reaction including the formation of a tetrahedral enzyme-inhibitor adduct and the formation of the carbamyl-enzyme, inhibition kinetics
4-nitrophenyl-N-octyl carbamate
-
mechanism of inhibition by these pseudo-substrate inhibitors, via a 2-step reaction including the formation of a tetrahedral enzyme-inhibitor adduct and the formation of the carbamyl-enzyme, inhibition kinetics
5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-6,7-diyl phosphoric acid tetraethyl ester
-
-
5-[3,5-bis[3-(1H-imidazol-5-yl)propyl]-1,2-oxazol-4-yl]-3-[[(4-chlorophenyl)sulfonyl]methyl]-1,2,4-oxadiazole
-
-
6-chloro-3-(1-ethyl-2-cyclohexyl)-2-pyrone
-
selective, potent, and reversible inhibitor, not toxic in vivo, enzyme inhibition effectively reduces cholesterol uptake in vivo
7-chloro-1-ethoxy-3-(4-ethylphenyl)-4-(phenylethynyl)-1H-2,1-benzoxaphosphinine 1-oxide
-
-
7-chloro-1-ethoxy-4-iodo-3-(4-methoxyphenyl)-1H-2,1-benzoxaphosphinine 1-oxide
-
-
albumin
-
50% inhibition by addition of 0.015-0.045 mg, increasing inhibition after heat treatment of albumin
-
amlodipine
-
mixed-type, study on kinetics in presence of Triton X-100 or taurocholate
AS115
nonspecific inhibitor, the enzyme is about 85% inhibited by 0.01 mM AS115
besylate
-
mixed-type, study on kinetics in presence of Triton X-100 or taurocholate
bisphenol A dimethacrylate
-
computational study on inhibitory effect. Bisphenol A dimethacrylate mimics chemical properties of reported enzyme inhibitors, particularly concerning maximum oxygen valency and molecular shape/size
brefeldin A
-
inhibition to 80%-90% of secreted enzyme into the medium of HepG2 cells
cardiolipin
-
inhibitory if included as aqueous sonicated dispersions at 2.5 mg/ml
cholesteryl esters
-
inhibition at 0.2 mM with cholesteryl-stearate, -oleate, -eicosenoate, -arachidonate, -erucate and -nervonate
heparin
-
50% inhibition for CEase-ISF II at 0.025 mM, no inhibition for CEase-IF I
hydralazine hydrochloride
-
mixed-type, study on kinetics in presence of Triton X-100 or taurocholate
lovastatin
-
mixed-type, study on kinetics in presence of Triton X-100 or taurocholate
methyl 3-[(2-ethoxy-2-oxoethyl)(methyl)sulfamoyl]-5-[2-(1H-pyrrol-2-yl)ethyl]thiophene-2-carboxylate
-
-
monensin
-
inhibition to 50%-60% of secreted enzyme into the medium of HepG2 cells
nifedipine
-
mixed-type, study on kinetics in presence of Triton X-100 or taurocholate
phosphatidylinositol
-
inhibitory if included as aqueous sonicated dispersions at 2.5 mg/ml
simvastatin
-
mixed-type, study on kinetics in presence of Triton X-100 or taurocholate
2-naphthyl-N-butyl carbamate
-
biphasic time course, reversible non-covalent enzyme-carbamate complex
cytosolic protein factor
-
sexual differences in level of inhibitory activity, pregnancy and lactation lead to decreased activity of the inhibitory protein, level of inhibitory protein responses to hormonal and nutritional status of females
-