BRENDA - Enzyme Database
show all sequences of 2.9.1.1

Towards understanding selenocysteine incorporation into bacterial proteins

Fischer, N.; Paleskava, A.; Gromadski, K.B.; Konevega, A.L.; Wahl, M.C.; Stark, H.; Rodnina, M.V.; Biol. Chem. 388, 1061-1067 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
selA, expression in Escherichia coli strain DH5alpha
Moorella thermoacetica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics study of selenocysteine incorporation
Moorella thermoacetica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-seryl-tRNASec + selenophosphate
Moorella thermoacetica
the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview
L-selenocysteinyl-tRNASec + phosphate
-
-
?
additional information
Moorella thermoacetica
SelA interacts with the transcription factor SelB, mutational interaction study, overview
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Moorella thermoacetica
-
gene selA
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant SelA from Escherichia coli strain DH5alpha by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration
Moorella thermoacetica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-seryl-tRNASec + selenophosphate
-
691146
Moorella thermoacetica
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
L-seryl-tRNASec + selenophosphate
the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview
691146
Moorella thermoacetica
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
additional information
SelA interacts with the transcription factor SelB, mutational interaction study, overview
691146
Moorella thermoacetica
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
decamer
the homodecamer has a ring-like structure with five bilobed wings, similar to the structure of the Escherichia coli complex
Moorella thermoacetica
More
formation of the ternary enzyme complex and binding to the ribosome, structure analysis by electron microscopy, 5fold rotational symmetry, overview
Moorella thermoacetica
Synonyms
Synonyms
Commentary
Organism
SelA
-
Moorella thermoacetica
Selenocysteine synthase
-
Moorella thermoacetica
Cloned(Commentary) (protein specific)
Commentary
Organism
selA, expression in Escherichia coli strain DH5alpha
Moorella thermoacetica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics study of selenocysteine incorporation
Moorella thermoacetica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-seryl-tRNASec + selenophosphate
Moorella thermoacetica
the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview
L-selenocysteinyl-tRNASec + phosphate
-
-
?
additional information
Moorella thermoacetica
SelA interacts with the transcription factor SelB, mutational interaction study, overview
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant SelA from Escherichia coli strain DH5alpha by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration
Moorella thermoacetica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-seryl-tRNASec + selenophosphate
-
691146
Moorella thermoacetica
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
L-seryl-tRNASec + selenophosphate
the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview
691146
Moorella thermoacetica
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
additional information
SelA interacts with the transcription factor SelB, mutational interaction study, overview
691146
Moorella thermoacetica
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
decamer
the homodecamer has a ring-like structure with five bilobed wings, similar to the structure of the Escherichia coli complex
Moorella thermoacetica
More
formation of the ternary enzyme complex and binding to the ribosome, structure analysis by electron microscopy, 5fold rotational symmetry, overview
Moorella thermoacetica
Other publictions for EC 2.9.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738683
Manhas
Leishmania donovani encodes a ...
Leishmania donovani
J. Biol. Chem.
291
1203-1220
2016
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725024
Manzine
Assembly stoichiometry of bact ...
Escherichia coli
FEBS Lett.
587
906-911
2013
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739586
Manzine
An efficient protocol for the ...
Escherichia coli
Protein Expr. Purif.
88
80-84
2013
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739706
Itoh
Decameric SelA-tRNA(Sec) ring ...
Aquifex aeolicus
Science
340
75-78
2013
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693212
Ganichkin
Structure and catalytic mechan ...
Mus musculus
J. Biol. Chem.
283
5849-5865
2008
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691146
Fischer
Towards understanding selenocy ...
Moorella thermoacetica
Biol. Chem.
388
1061-1067
2007
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691179
Xu
New developments in selenium b ...
Homo sapiens, Mus musculus
Biol. Trace Elem. Res.
119
234-241
2007
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660828
Gursinsky
A selDABC cluster for selenocy ...
Peptoclostridium acidaminophilum
Arch. Microbiol.
174
200-212
2000
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661290
Mizutani
Selenophosphate as a substrate ...
Bos taurus
Biofactors
9
27-36
1999
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22917
Tormay
Bacterial selenocysteine synth ...
Desulfomicrobium baculatum, Escherichia coli, Haemophilus influenzae, Moorella thermoacetica
Eur. J. Biochem.
254
655-661
1998
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2
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22916
Tanabe
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Selenophosphate as a substrate ...
Bos taurus
Nucleic acids Symp. Ser.
35
287-288
1996
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22915
Engelhardt
Structure of selenocysteine sy ...
Escherichia coli
Mol. Microbiol.
6
3461-3467
1992
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22912
Forchhammer
Selenocysteine synthase from E ...
Escherichia coli
J. Biol. Chem.
266
6324-6328
1991
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22913
Forchhammer
Selenocysteine synthase from E ...
Escherichia coli
J. Biol. Chem.
266
6318-6323
1991
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22914
Forchhammer
The function of selenocysteine ...
Escherichia coli
Biochimie
73
1481-1486
1991
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