Literature summary for 2.8.4.1 extracted from

Lyu, Z.; Shao, N.; Chou, C.W.; Shi, H.; Patel, R.; Duin, E.C.; Whitman, W.B.
Posttranslational methylation of arginine in methyl coenzyme M reductase has a profound impact on both methanogenesis and growth of Methanococcus maripaludis (2020), J. Bacteriol., 202, e00654-19 .

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Application

Application	Comment	Organism
biofuel production	CH4 is an important biofuel as well as a potential feedstock for the chemical industry if it can be converted by Mcr to a liquid biofuel with a high energy density	Methanococcus maripaludis
environmental protection	metabolization of methane can positively influence the environment	Methanococcus maripaludis

Protein Variants

Protein Variants	Comment	Organism
additional information	markerless deletion of methanogenesis marker protein 10 (Mmp10), encoded by gene mmpX, can play an important role in methanogenesis. To systematically examine its physiological role, mmpX (locus tag MMP1554), the deletion of gene encoding Mmp10 in Methanococcus maripaludis results in the complete loss of the 5-C-(S)-methylarginine PTM of residue 275 in the McrA subunit. When the DELTAmmpX mutant is complemented with the wild-type gene expressed by either a strong or a weak promoter, methylation is fully restored. Compared to the parental strain, maximal rates of methane formation by whole cells are reduced by 40 to 60% in the DELTAmmpX mutant. The reduction in activity is fully reversed by the complement with the strong promoter. Site-directed mutagenesis of mmpX result in a differential loss of arginine methylation among the mutants in vivo. Construction of diverse mutant strains from parental strain S0001, and phenotypes, overview	Methanococcus maripaludis

General Stability

General Stability	Organism
over 90% of the activity of resting cells harboring enzyme Mcr is lost within 1 h, Mcr from Methanococcus maripaludis is very unstable even in cell extracts	Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
methyl-CoM + CoB	Methanococcus maripaludis	-	CoM-S-S-CoB + methane	-	r
methyl-CoM + CoB	Methanococcus maripaludis LL	-	CoM-S-S-CoB + methane	-	r

Organism

Organism	UniProt	Comment	Textmining
Methanococcus maripaludis	Q6LWZ5 AND Q6LWZ9 AND Q6LWZ6	encoding subunit A, B, and G	-
Methanococcus maripaludis LL	Q6LWZ5 AND Q6LWZ9 AND Q6LWZ6	encoding subunit A, B, and G	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
alkylation	the posttranslational methylation of arginine in methyl coenzyme M reductase has a profound impact on both methanogenesis and growth of Methanococcus maripaludis. Protein Mmp10 is necessary for arginine methylation. Replacement of the only conserved His residue in the C-terminal half of Mmp10 with either G or F results in a loss of less than 1% of Arg275 methylation	Methanococcus maripaludis
additional information	the McrA subunit possesses up to five highly conserved but not universal posttranslational modifications (PTMs) at its active site, including 1-N-methyl-His261, 5-C-(S)-methyl-Arg275, 2-(S)-methyl-Gln403, and thio-Gly448, which are all found within the McrA subunit	Methanococcus maripaludis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl-CoM + CoB	-	Methanococcus maripaludis	CoM-S-S-CoB + methane	-	r
methyl-CoM + CoB	-	Methanococcus maripaludis LL	CoM-S-S-CoB + methane	-	r

Subunits

Subunits	Comment	Organism
heterotrimer	-	Methanococcus maripaludis

Synonyms

Synonyms	Comment	Organism
MCR	-	Methanococcus maripaludis
mcrA	subunit A	Methanococcus maripaludis
mcrB	subunit B	Methanococcus maripaludis
mcrG	subunit G	Methanococcus maripaludis
MMP1555	-	Methanococcus maripaludis
MMP1558	-	Methanococcus maripaludis
MMP1559	-	Methanococcus maripaludis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Methanococcus maripaludis

Cofactor

Cofactor	Comment	Organism	Structure
F-430	coenzyme F430, during enzymatic catalysis, the Ni(I) of coenzyme F430 seen to attack the sulfur atom of methyl coenzyme M, producing a methyl radical intermediate	Methanococcus maripaludis

General Information

General Information	Comment	Organism
additional information	the enzyme possesses two identical active sites, each of which contains up to five posttranslationally modified amino acid residues. Role of Mmp10 in Mcr methylation on Arg275 and function in methogenesis,mutational analysis, overview	Methanococcus maripaludis
physiological function	roles of Mcr in the global carbon cycle. CH4 is an important biofuel as well as a potential feedstock for the chemical industry if it can be converted by Mcr to a liquid biofuel with a high energy density. CH4 is also a potent greenhouse gas, increases of which are contributing to global warming	Methanococcus maripaludis

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Release 2023.1 (January 2023)