Cloned (Comment) | Organism |
---|---|
overproduction in Escherichia coli | Acetobacterium woodii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
3,4-dihydroxyphenylpropanoyl-CoA | pH 7.5, 40°C | Acetobacterium woodii | |
0.075 | - |
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate | pH 7.5, 40°C | Acetobacterium woodii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Acetobacterium woodii |
130000 | - |
gel filtration | Acetobacterium woodii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylpropanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate | Acetobacterium woodii | the enzyme catalyzes an energy-saving CoA loop for caffeate activation. 3-(3,4-dihydroxyphenyl)acrylate i.e. caffeate | 3,4-dihydroxyphenylpropanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacterium woodii | F1CYZ5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetobacterium woodii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-(3,4-dihydroxyphenyl)propanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate = 3-(3,4-dihydroxyphenyl)propanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA | the enzyme uses a ping-pong mechanism for CoA transfer, it has two catalytic domains on one polypeptide chain. (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate = caffeate. 3,4-dihydroxyphenylpropanoate = hydrocaffeate | Acetobacterium woodii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylpropanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate | the enzyme catalyzes an energy-saving CoA loop for caffeate activation. 3-(3,4-dihydroxyphenyl)acrylate i.e. caffeate | Acetobacterium woodii | 3,4-dihydroxyphenylpropanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA | - |
? | |
3,4-dihydroxyphenylpropanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate | 84% compared to the activity with 4-coumarate. (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate i.e. caffeate. 3,4-dihydroxyphenylpropanoate i.e. hydrocaffeate | Acetobacterium woodii | 3,4-dihydroxyphenylpropanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA | - |
? | |
3,4-dihydroxyphenylpropanoyl-CoA + 4-coumarate | 3,4-dihydroxyphenylpropanoyl-CoA i.e. hydrocaffeoyl-CoA | Acetobacterium woodii | 3,4-dihydroxyphenylpropanoate + 4-coumaroyl-CoA | - |
? | |
3,4-dihydroxyphenylpropanoyl-CoA + ferulate | 86% compared to the activity with 4-coumarate | Acetobacterium woodii | 3,4-dihydroxyphenylpropanoate + feruloyl-CoA | - |
? | |
additional information | no activity with cinnamate, sinapate, or 4-hydroxybenzoate as a CoA acceptor. Neither acetyl-CoA nor butanoyl-CoA serve as the CoA donor for the enzyme | Acetobacterium woodii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, SDS-PAGE | Acetobacterium woodii |
Synonyms | Comment | Organism |
---|---|---|
CarA | - |
Acetobacterium woodii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Acetobacterium woodii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
3,4-dihydroxyphenylpropanoyl-CoA | pH 7.5, 40°C | Acetobacterium woodii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Acetobacterium woodii |