Information on EC 2.8.3.23 - caffeate CoA-transferase

for references in articles please use BRENDA:EC2.8.3.23
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The expected taxonomic range for this enzyme is: Acetobacterium woodii

EC NUMBER
COMMENTARY hide
2.8.3.23
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RECOMMENDED NAME
GeneOntology No.
caffeate CoA-transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-(3,4-dihydroxyphenyl)propanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate = 3-(3,4-dihydroxyphenyl)propanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
3-(3,4-dihydroxyphenyl)propanoyl-CoA:(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate CoA-transferase
The enzyme, isolated from the bacterium Acetobacterium woodii, catalyses an energy-saving CoA loop for caffeate activation. In addition to caffeate, the enzyme can utilize 4-coumarate or ferulate as CoA acceptor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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coenzyme A transferase CarA, acyl-CoA synthetase CarB, and acyl-CoA dehydrogenase CarC are part of the caffeate respiration pathway
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxyphenylpropanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate
3,4-dihydroxyphenylpropanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA
show the reaction diagram
3,4-dihydroxyphenylpropanoyl-CoA + 4-coumarate
3,4-dihydroxyphenylpropanoate + 4-coumaroyl-CoA
show the reaction diagram
3,4-dihydroxyphenylpropanoyl-CoA i.e. hydrocaffeoyl-CoA
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-
?
3,4-dihydroxyphenylpropanoyl-CoA + ferulate
3,4-dihydroxyphenylpropanoate + feruloyl-CoA
show the reaction diagram
86% compared to the activity with 4-coumarate
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?
additional information
?
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no activity with cinnamate, sinapate, or 4-hydroxybenzoate as a CoA acceptor. Neither acetyl-CoA nor butanoyl-CoA serve as the CoA donor for the enzyme
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxyphenylpropanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate
3,4-dihydroxyphenylpropanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA
show the reaction diagram
F1CYZ5
the enzyme catalyzes an energy-saving CoA loop for caffeate activation. 3-(3,4-dihydroxyphenyl)acrylate i.e. caffeate
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.075
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate
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pH 7.5, 40°C
0.008
3,4-dihydroxyphenylpropanoyl-CoA
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pH 7.5, 40°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.008
3,4-dihydroxyphenylpropanoyl-CoA
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pH 7.5, 40°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57700
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x * 57700, calculated
60000
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2 * 60000, SDS-PAGE
130000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 57700, calculated
homodimer
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2 * 60000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overproduction in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of the car operon is induced by caffeate, p-coumarate, ferulate, and cinnamate and also by sinapate
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there is no induction by p-hydroxybenzoate or syringate
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