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Literature summary for 2.8.3.14 extracted from

  • Eikmanns, U.; Buckel, W.
    Properties of 5-hydroxyvalerate CoA-transferase from Clostridium aminovalericum (1990), Biol. Chem. Hoppe-Seyler, 371, 1077-1082.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP slightly Anaerocolumna aminovalerica
ATP
-
Anaerocolumna aminovalerica
CTP
-
Anaerocolumna aminovalerica
GTP slightly Anaerocolumna aminovalerica
additional information not inhibited by AMP Anaerocolumna aminovalerica
Sodium boranate only in presence of a CoA substrate Anaerocolumna aminovalerica
UTP slightly Anaerocolumna aminovalerica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
5-hydroxypentanoyl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
0.009
-
propionyl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
0.018
-
acetyl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
0.029
-
butyryl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
0.03
-
(Z)-5-hydroxypent-2-enoyl-CoA pH 8.0, 25°C, cosubstrate acetate Anaerocolumna aminovalerica
0.037
-
pentanoyl-CoA pH 8.0, 25°C, cosubstrate (Z)-5-hydroxy-2-pentenoate Anaerocolumna aminovalerica
0.07
-
butyryl-CoA pH 8.0, 25°C, cosubstrate acetate Anaerocolumna aminovalerica
0.1
-
5-hydroxypentanoyl-CoA pH 8.0, 25°C, cosubstrate acetate Anaerocolumna aminovalerica
0.12
-
propionyl-CoA pH 8.0, 25°C, acetate Anaerocolumna aminovalerica
0.45
-
pentanoyl-CoA pH 8.0, 25°C, cosubstrate acetate Anaerocolumna aminovalerica
4
-
(Z)-5-hydroxy-2-pentenoate pH 8.0, 25°C, cosubstrate acetyl-CoA Anaerocolumna aminovalerica
20
-
acetate pH 8.0, 25°C, cosubstrate propionyl-CoA Anaerocolumna aminovalerica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
4 * 47000, homotetramer, SDS-PAGE Anaerocolumna aminovalerica
155000 160000 gradient PAGE Anaerocolumna aminovalerica
178000
-
average molecular mass Anaerocolumna aminovalerica
180000 195000 gel filtration, Superose 6 Anaerocolumna aminovalerica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-hydroxypentanoyl-CoA + acetate Anaerocolumna aminovalerica
-
acetyl-CoA + 5-hydroxypentanoate
-
?
5-hydroxypentanoyl-CoA + acetate Anaerocolumna aminovalerica T2-7
-
acetyl-CoA + 5-hydroxypentanoate
-
?

Organism

Organism UniProt Comment Textmining
Anaerocolumna aminovalerica
-
T2-7
-
Anaerocolumna aminovalerica T2-7
-
T2-7
-

Purification (Commentary)

Purification (Comment) Organism
-
Anaerocolumna aminovalerica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38 46
-
Anaerocolumna aminovalerica

Storage Stability

Storage Stability Organism
-20°C, no loss of activity observed after storage for several months Anaerocolumna aminovalerica
4°C, lose 50% of its activity within 2 months when kept in 20 mM potassium phosphate buffer, pH 6.8 Anaerocolumna aminovalerica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxypentanoyl-CoA + acetate
-
Anaerocolumna aminovalerica acetyl-CoA + 5-hydroxypentanoate
-
?
5-hydroxypentanoyl-CoA + acetate
-
Anaerocolumna aminovalerica T2-7 acetyl-CoA + 5-hydroxypentanoate
-
?
acetate + (Z)-5-hydroxypent-2-enoyl-CoA
-
Anaerocolumna aminovalerica acetyl-CoA + (Z)-5-hydroxypent-2-enoate
-
?
acetyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
Anaerocolumna aminovalerica acetate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
r
acetyl-CoA + 3-pentenoate
-
Anaerocolumna aminovalerica acetate + 3-pentenoyl-CoA
-
?
acetyl-CoA + 4-pentenoate
-
Anaerocolumna aminovalerica acetate + 4-pentenoyl-CoA
-
?
acetyl-CoA + 5-hydroxypentanoate
-
Anaerocolumna aminovalerica acetate + 5-hydroxypentanoyl-CoA
-
r
acetyl-CoA + acetate
-
Anaerocolumna aminovalerica acetyl-CoA + acetate
-
?
acetyl-CoA + acetate
-
Anaerocolumna aminovalerica T2-7 acetyl-CoA + acetate
-
?
acetyl-CoA + propionate
-
Anaerocolumna aminovalerica acetate + propanoyl-CoA
-
r
butyryl-CoA + (Z)-5-hydroxy-2-pentenoate
-
Anaerocolumna aminovalerica butyrate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
?
butyryl-CoA + acetate
-
Anaerocolumna aminovalerica acetyl-CoA + butanoate
-
?
butyryl-CoA + acetate
-
Anaerocolumna aminovalerica T2-7 acetyl-CoA + butanoate
-
?
additional information all substrates are bond in a (Z)-2,3-conformation to the enzyme, crotonate, (E)-5-hydroxy-2-pentenoate, (E)-2-pentenoate and 2,4-pentadienoate are no substrates Anaerocolumna aminovalerica ?
-
?
additional information all substrates are bond in a (Z)-2,3-conformation to the enzyme, crotonate, (E)-5-hydroxy-2-pentenoate, (E)-2-pentenoate and 2,4-pentadienoate are no substrates Anaerocolumna aminovalerica T2-7 ?
-
?
pentanoyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
Anaerocolumna aminovalerica pentanoate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
?
pentanoyl-CoA + acetate
-
Anaerocolumna aminovalerica acetyl-CoA + pentanoate
-
?
propionyl-CoA + (Z)-5-hydroxy-2-pentenoate
-
Anaerocolumna aminovalerica propionate + (Z)-5-hydroxy-2-pentenoyl-CoA
-
?
propionyl-CoA + acetate
-
Anaerocolumna aminovalerica acetyl-CoA + propanoate
-
r
propionyl-CoA + acetate
-
Anaerocolumna aminovalerica T2-7 acetyl-CoA + propanoate
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 47000, homotetramer, SDS-PAGE Anaerocolumna aminovalerica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3
-
ATP pH 8.0, 25°C substrate acetate, non-competitive inhibition Anaerocolumna aminovalerica
3
-
ATP pH 8.0, 25°C substrate propionyl-CoA, competitive inhibition Anaerocolumna aminovalerica
5
-
ATP pH 8.0, 25°C substrate 5-hydroxypentanoyl-CoA, competitive inhibition Anaerocolumna aminovalerica