Information on EC 2.8.3.14 - 5-hydroxypentanoate CoA-transferase

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The expected taxonomic range for this enzyme is: Anaerocolumna aminovalerica

EC NUMBER
COMMENTARY hide
2.8.3.14
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RECOMMENDED NAME
GeneOntology No.
5-hydroxypentanoate CoA-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + 5-hydroxypentanoate = acetate + 5-hydroxypentanoyl-CoA
show the reaction diagram
propanoyl-CoA, acetyl-CoA, butanoyl-CoA and some other acyl-CoAs can act as substrates, but more slowly than 5-hydroxypentanoyl-CoA
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:5-hydroxypentanoate CoA-transferase
Propanoyl-CoA, acetyl-CoA, butanoyl-CoA and some other acyl-CoAs can act as substrates, but more slowly than 5-hydroxypentanoyl-CoA.
CAS REGISTRY NUMBER
COMMENTARY hide
111684-68-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-hydroxypentanoyl-CoA + acetate
acetyl-CoA + 5-hydroxypentanoate
show the reaction diagram
acetyl-CoA + (Z)-5-hydroxy-2-pentenoate
acetate + (Z)-5-hydroxy-2-pentenoyl-CoA
show the reaction diagram
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-
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r
acetyl-CoA + 3-pentenoate
acetate + 3-pentenoyl-CoA
show the reaction diagram
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-
-
?
acetyl-CoA + 4-pentenoate
acetate + 4-pentenoyl-CoA
show the reaction diagram
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-
-
?
acetyl-CoA + 5-hydroxypentanoate
acetate + 5-hydroxypentanoyl-CoA
show the reaction diagram
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-
-
r
acetyl-CoA + acetate
acetyl-CoA + acetate
show the reaction diagram
acetyl-CoA + propionate
acetate + propanoyl-CoA
show the reaction diagram
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-
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r
butyryl-CoA + (Z)-5-hydroxy-2-pentenoate
butyrate + (Z)-5-hydroxy-2-pentenoyl-CoA
show the reaction diagram
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-
-
-
?
butyryl-CoA + acetate
acetyl-CoA + butanoate
show the reaction diagram
pentanoyl-CoA + (Z)-5-hydroxy-2-pentenoate
pentanoate + (Z)-5-hydroxy-2-pentenoyl-CoA
show the reaction diagram
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?
pentanoyl-CoA + acetate
acetyl-CoA + pentanoate
show the reaction diagram
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-
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?
propionyl-CoA + (Z)-5-hydroxy-2-pentenoate
propionate + (Z)-5-hydroxy-2-pentenoyl-CoA
show the reaction diagram
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-
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?
propionyl-CoA + acetate
acetyl-CoA + propanoate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-hydroxypentanoyl-CoA + acetate
acetyl-CoA + 5-hydroxypentanoate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Sodium boranate
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only in presence of a CoA substrate
additional information
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not inhibited by AMP
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
(Z)-5-hydroxy-2-pentenoate
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pH 8.0, 25C, cosubstrate acetyl-CoA
0.03
(Z)-5-hydroxypentenoyl-CoA
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pH 8.0, 25C, cosubstrate acetate
0.0038 - 0.1
5-hydroxypentanoyl-CoA
20
acetate
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pH 8.0, 25C, cosubstrate propionyl-CoA
0.018
acetyl-CoA
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pH 8.0, 25C, cosubstrate (Z)-5-hydroxy-2-pentenoate
0.029 - 0.07
butyryl-CoA
0.037 - 0.45
pentanoyl-CoA
0.009 - 0.12
propionyl-CoA
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3 - 5
ATP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
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4 * 47000, homotetramer, SDS-PAGE
155000 - 160000
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gradient PAGE
178000
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average molecular mass
180000 - 195000
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gel filtration, Superose 6
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, no loss of activity observed after storage for several months
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4C, lose 50% of its activity within 2 months when kept in 20 mM potassium phosphate buffer, pH 6.8
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE