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Literature summary for 2.8.2.4 extracted from
- Characteristics and behavior during partial purification of estrogen sulfotransferase of guinea pig liver and chorion (1987), Biochim. Biophys. Acta, 925, 362-370.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| monothiolglycerol | activity is stimulated 10fold by 25 mM | Cavia porcellus |  |
General Stability
| General Stability | Organism |
|---|---|
| not stabilized by 20% glycerol or 25 mM NaCl | Cavia porcellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ZnCl2 | inhibition of liver isoenzyme at 15 mM | Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Cavia porcellus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activity is stimulated 2fold by 15 mM CaCl2 | Cavia porcellus | |
| Mg2+ | activity is stimulated 2fold by 15 mM MgCl2 | Cavia porcellus | |
| Zn2+ | weakly stimulatory at 2.5 mM ZnCl2 | Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
chorion enzyme, gel filtration | Cavia porcellus |
| 52300 | - |
liver enzyme, gel filtration | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + estradiol | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + estrone | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
guinea pig | - |
| Cavia porcellus | - |
english shorthair | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, liver and chorion isoenzymes | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| chorion | - |
Cavia porcellus | - |
| liver | - |
Cavia porcellus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 25 mM monothiolglycerol, 20% loss of activity in 1 week | Cavia porcellus |
| -20°C, partially purified enzyme exhibits large losses of activity within 1 week | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + estradiol | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + estrone | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + estrone 3-sulfate | - |
r | |
| additional information | activity toward dehydroepiandrosterone and testosterone is minimal or undetectable | Cavia porcellus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
liver isoenzyme | Cavia porcellus |
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