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Literature summary for 2.8.2.32 extracted from
- Identification and characterization of 5alpha-cyprinol-sulfating cytosolic sulfotransferases (Sults) in the zebrafish (Danio rerio) (2017), J. Steroid Biochem. Mol. Biol., 174, 120-127 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5alpha-cyprinol | substrate inhibition; substrate inhibition; substrate inhibition kinetics | Danio rerio |  |
| 5alpha-cyprinol | substrate inhibition kinetics | Homo sapiens | |
| 5beta-cyprinol | substrate inhibition; substrate inhibition | Danio rerio | |
| additional information | the sulfation of both of 5alpha-cyprinol and 5beta-cyprinol by zebrafish Sult2st2 and Sult2st3 can be fitted to substrate inhibition kinetics; the sulfation of both of 5alpha-cyprinol and 5beta-cyprinol by zebrafish Sult2st2 and Sult2st3 can be fitted to substrate inhibition kinetics | Danio rerio |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, kinetic analysis | Danio rerio | |
| additional information | - |
additional information | Michaelis-Menten kinetics for beta-cyprinol, kinetic analysis | Homo sapiens | |
| additional information | - |
additional information | Michaelis-Menten kinetics for beta-cyprinol, kinetic analysis | Danio rerio |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Danio rerio | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + 5alpha-cyprinol | Homo sapiens | - |
adenosine 3',5'-bisphosphate + 5alpha-cyprinol sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + 5alpha-cyprinol | Danio rerio | - |
adenosine 3',5'-bisphosphate + 5alpha-cyprinol sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + 5beta-cyprinol | Homo sapiens | - |
adenosine 3',5'-bisphosphate + 5beta-cyprinol sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + 5beta-cyprinol | Danio rerio | - |
adenosine 3',5'-bisphosphate + 5beta-cyprinol sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | A0A0R4IT32 | - |
- |
| Danio rerio | A4FUP0 | - |
- |
| Danio rerio | Q1L874 | - |
- |
| Homo sapiens | Q06520 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00034 | - |
substrate 5beta-cyprinol, pH 7.0, 37°C, purified recombinant enzyme | Homo sapiens |
| 0.00115 | - |
substrate 5beta-cyprinol, pH 7.0, 28°C, purified recombinant enzyme | Danio rerio |
| 0.00143 | - |
substrate 5alpha-cyprinol, pH 7.0, 28°C, purified recombinant enzyme | Danio rerio |
| 0.00471 | - |
substrate 5alpha-cyprinol, pH 7.0, 37°C, purified recombinant enzyme | Homo sapiens |
| 0.01008 | - |
substrate 5beta-cyprinol, pH 7.0, 28°C, purified recombinant enzyme | Danio rerio |
| 0.01014 | - |
substrate 5alpha-cyprinol, pH 7.0, 28°C, purified recombinant enzyme | Danio rerio |
| 0.02642 | - |
substrate 5beta-cyprinol, pH 7.0, 28°C, purified recombinant enzyme | Danio rerio |
| 0.02768 | - |
substrate 5alpha-cyprinol, pH 7.0, 28°C, purified recombinant enzyme | Danio rerio |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + 5alpha-cyprinol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 5alpha-cyprinol sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + 5alpha-cyprinol | - |
Danio rerio | adenosine 3',5'-bisphosphate + 5alpha-cyprinol sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + 5alpha-cyprinol | 3alpha,7alpha,12alpha,26,27-pentahydroxy-5alpha-cholestane | Homo sapiens | adenosine 3',5'-bisphosphate + 5alpha-cyprinol sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + 5alpha-cyprinol | 3alpha,7alpha,12alpha,26,27-pentahydroxy-5alpha-cholestane | Danio rerio | adenosine 3',5'-bisphosphate + 5alpha-cyprinol sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + 5beta-cyprinol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + 5beta-cyprinol sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + 5beta-cyprinol | - |
Danio rerio | adenosine 3',5'-bisphosphate + 5beta-cyprinol sulfate | - |
? | |
| additional information | NMR and mass spectrometry analysis of the reaction products. Human SULT2A1 shows substrate inhibition kinetics for 5alpha-cyprinol and Michaelis-Menten kinetics for beta-cyprinol. High activity with dehydroepiandrosterone (DHEA), cf. EC 2.8.2.2 | Homo sapiens | ? | - |
- |
|
| additional information | NMR and mass spectrometry analysis of the reaction products. The sulfation of both of 5alpha-cyprinol and 5beta-cyprinol by zebrafish Sult2st2 and Sult2st3 can be fitted to substrate inhibition kinetics. No activity with 3alpha,7alpha,12alpha-trihydroxy-5alpha-cholestanoic acid (5alpha-TriOH CA), dehydroepiandrosterone (DHEA) is a poor substrate | Danio rerio | ? | - |
- |
|
| additional information | NMR and mass spectrometry analysis of the reaction products. Zebrafish Sult3st4 shows substrate inhibition kinetics for 5alpha-cyprinol and Michaelis-Menten kinetics for beta-cyprinol. High activity with dehydroepiandrosterone (DHEA), cf. EC 2.8.2.2 | Danio rerio | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5alpha-cyprinol-sulfating cytosolic sulfotransferase | - |
Homo sapiens |
| 5alpha-cyprinol-sulfating cytosolic sulfotransferase | - |
Danio rerio |
| 5alpha-cyprinol-sulfating Sult | - |
Homo sapiens |
| 5alpha-cyprinol-sulfating Sult | - |
Danio rerio |
| More | cf. EC 2.8.2.2 | Homo sapiens |
| SULT2A1 | - |
Homo sapiens |
| sult2st2 | - |
Danio rerio |
| sult2st3 | - |
Danio rerio |
| Sult3st4 | - |
Danio rerio |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Danio rerio |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Danio rerio |
| 7.5 | 9.5 | broad optimum | Homo sapiens |
| 8.5 | - |
- |
Danio rerio |
| 9.5 | - |
- |
Danio rerio |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | activity range of Sult2st3 | Danio rerio |
| 6 | 10.5 | activity range, high activity within this range | Danio rerio |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | both Sult2st2 and Sult2st3 have evolved to sulfate specifically C27-bile alcohol, 5alpha-cyprinol, in Cypriniform fish, whereas the enzymatic characteristics of zebrafish Sult3 members, particularly Sult3st4, correlate with those of human SULT2A1. Whereas zebrafish Sult2 family members are active in the sulfation of bile alcohols, Sult3 family members sulfate 3beta-hydroxysteroids such as pregnenolone and DHEA | Danio rerio |
| evolution | both Sult2st2 and Sult2st3 have evolved to sulfate specifically C27-bile alcohol, 5alpha-cyprinol, in Cypriniform fish, whereas the enzymatic characteristics of zebrafish Sult3 members, particularly Sult3st4, correlate with those of human SULT2A1. Whereas zebrafish Sult2 family members are active in the sulfation of bile alcohols, Sult3 family members sulfate 3beta-hydroxysteroids such as pregnenolone and DHEA. Substrate recognition sites of zebrafish Sult2 and Sult3 family members differ significantly from those of human SULT2A1 | Homo sapiens |
| evolution | both Sult2st2 and Sult2st3 have evolved to sulfate specifically C27-bile alcohol, 5alpha-cyprinol, in Cypriniform fish, whereas the enzymatic characteristics of zebrafish Sult3 members, particularly Sult3st4, correlate with those of human SULT2A1. Whereas zebrafish Sult2 family members are active in the sulfation of bile alcohols, Sult3 family members sulfate 3beta-hydroxysteroids such as pregnenolone and DHEA. Substrate recognition sites of zebrafish Sult2 and Sult3 family members differ significantly from those of human SULT2A1 | Danio rerio |
| physiological function | Sult2st2 and Sult2st3 are the major 5alpha-cyprinol-sulfating Sults in zebrafish that sulfate 5alpha-cyprinol at C27 position. Both Sult2st2 and Sult2st3 apparently do not distinguish between 5alpha-cyprinol and 5beta-cyprinol, two bile salts with opposite A/B ring junctions | Homo sapiens |
| physiological function | Sult2st2 and Sult2st3 are the major 5alpha-cyprinol-sulfating Sults in zebrafish that sulfate 5alpha-cyprinol at C27 position. Both Sult2st2 and Sult2st3 apparently do not distinguish between 5alpha-cyprinol and 5beta-cyprinol, two bile salts with opposite A/B ring junctions | Danio rerio |
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