Application | Comment | Organism |
---|---|---|
drug development | introduction of a single sulfate group into the heparin octasaccharide is sufficient for effective blocking of HSV-1 infection | Human alphaherpesvirus 1 |
Cloned (Comment) | Organism |
---|---|
expression of N-terminally His6-tagged 3-OST-3 catalytic domain, comprising residues Gly139-Gly406, in Escherichia coli | Human alphaherpesvirus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Human alphaherpesvirus 1 | heparan sulfate is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1 utilizes a specialized cell surface HS, known as 3-O-sulfated HS, as an entry receptor to establish infection. 3-O-sulfated octasaccharide has strong activity in blocking HSV-1 infection, stronger than that of the 3-OH octasaccharide, overview | ? | - |
? | |
additional information | Human alphaherpesvirus 1 HSV-1 | heparan sulfate is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1 utilizes a specialized cell surface HS, known as 3-O-sulfated HS, as an entry receptor to establish infection. 3-O-sulfated octasaccharide has strong activity in blocking HSV-1 infection, stronger than that of the 3-OH octasaccharide, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human alphaherpesvirus 1 | - |
HSV-1 | - |
Human alphaherpesvirus 1 HSV-1 | - |
HSV-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged 3-OST-3 catalytic domain, comprising residues Gly139-Gly406, from Escherichia coli | Human alphaherpesvirus 1 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | infection of human HeLa and cornea fibroblast cells | Human alphaherpesvirus 1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparin 3-hydroxy octasaccharide + 3'-phosphoadenylyl sulfate | i.e. 3-OH octasaccharide, from partially depolymerized heparin, substrate preparation: upscaling by by coupling 3-OST-3 modification with a PAPS regeneration system, overview. Determination of the site of 3-O-sulfation in the octasaccharide at the G6 residue | Human alphaherpesvirus 1 | 3-O-sulfated heparin octasaccharide + adenosine 3',5'-bisphosphate | i.e. DELTAUA2S-GlcNS6S-IdoUA2S-GlcNS6S-IdoUA2S-GlcNS3S6S-IdoUA2SGlcNS6S, where DELTAUA is 4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid, GlcN is D-glucosamine, and IdoUA is L-iduronic acid | ? | |
heparin 3-hydroxy octasaccharide + 3'-phosphoadenylyl sulfate | i.e. 3-OH octasaccharide, from partially depolymerized heparin, substrate preparation: upscaling by by coupling 3-OST-3 modification with a PAPS regeneration system, overview. Determination of the site of 3-O-sulfation in the octasaccharide at the G6 residue | Human alphaherpesvirus 1 HSV-1 | 3-O-sulfated heparin octasaccharide + adenosine 3',5'-bisphosphate | i.e. DELTAUA2S-GlcNS6S-IdoUA2S-GlcNS6S-IdoUA2S-GlcNS3S6S-IdoUA2SGlcNS6S, where DELTAUA is 4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid, GlcN is D-glucosamine, and IdoUA is L-iduronic acid | ? | |
additional information | heparan sulfate is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1 utilizes a specialized cell surface HS, known as 3-O-sulfated HS, as an entry receptor to establish infection. 3-O-sulfated octasaccharide has strong activity in blocking HSV-1 infection, stronger than that of the 3-OH octasaccharide, overview | Human alphaherpesvirus 1 | ? | - |
? | |
additional information | heparan sulfate is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1 utilizes a specialized cell surface HS, known as 3-O-sulfated HS, as an entry receptor to establish infection. 3-O-sulfated octasaccharide has strong activity in blocking HSV-1 infection, stronger than that of the 3-OH octasaccharide, overview | Human alphaherpesvirus 1 HSV-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-OST-3 | - |
Human alphaherpesvirus 1 |
HS 3-O-sulfotransferase isoform 3 | - |
Human alphaherpesvirus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Human alphaherpesvirus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Human alphaherpesvirus 1 |