Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is dependent on the presence of unprotonated amino groups | Cavia porcellus | |
additional information | activity is dependent on the presence of unprotonated amino groups | Rattus norvegicus | |
additional information | activity is dependent on the presence of unprotonated amino groups | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
3'-phosphoadenylylsulfate | with 2-naphthylamine as second substrate, pH 8, 37°C | Cavia porcellus | |
0.03 | - |
3'-phosphoadenylylsulfate | with 2-naphthylamine as second substrate, pH 8, 37°C | Rattus norvegicus | |
0.03 | - |
3'-phosphoadenylylsulfate | with 2-naphthylamine as second substrate, pH 8, 37°C | Oryctolagus cuniculus | |
14 | - |
1,2,3,4-tetrahydroisoquinoline | pH 8, 37°C | Cavia porcellus | |
38 | - |
1,2,3,4-tetrahydroquinoline | pH 8, 37°C | Cavia porcellus | |
3400 | - |
2-Naphthylamine | pH 8, 37°C | Cavia porcellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Cavia porcellus | |
Mg2+ | required | Rattus norvegicus | |
Mg2+ | required | Oryctolagus cuniculus | |
Mg2+ | optimal stimulation: 20 mM MgCl2 | Cavia porcellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000 + x * 34000, SDS-PAGE | Cavia porcellus |
34000 | - |
x * 33000 + x * 34000, SDS-PAGE | Cavia porcellus |
60000 | - |
gel filtration | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + 2-naphthylamine | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + 2-naphthylsulfamate | - |
ir | |
3'-phosphoadenylylsulfate + 2-naphthylamine | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + 2-naphthylsulfamate | - |
? | |
3'-phosphoadenylylsulfate + 2-naphthylamine | Oryctolagus cuniculus | - |
adenosine 3',5'-bisphosphate + 2-naphthylsulfamate | - |
? | |
3'-phosphoadenylylsulfate + aniline | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + N-sulfoaniline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Oryctolagus cuniculus | - |
rabbit | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Cavia porcellus | - |
intestine | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Cavia porcellus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Oryctolagus cuniculus | - |
mucosa | - |
Cavia porcellus | - |
mucosa | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
- |
Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + 1,2,3,4-tetrahydroisoquinoline | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + 1,2,3,4-tetrahydroisoquinoline N-sulfate | - |
? | |
3'-phosphoadenylylsulfate + 1,2,3,4-tetrahydroquinoline | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + 1,2,3,4-tetrahydroquinoline N-sulfate | - |
? | |
3'-phosphoadenylylsulfate + 2-naphthylamine | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + 2-naphthylsulfamate | - |
ir | |
3'-phosphoadenylylsulfate + 2-naphthylamine | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + 2-naphthylsulfamate | - |
? | |
3'-phosphoadenylylsulfate + 2-naphthylamine | - |
Oryctolagus cuniculus | adenosine 3',5'-bisphosphate + 2-naphthylsulfamate | - |
? | |
3'-phosphoadenylylsulfate + aniline | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + N-sulfoaniline | - |
? | |
3'-phosphoadenylylsulfate + cyclohexylamine | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + N-sulfocyclohexylamine | - |
? | |
3'-phosphoadenylylsulfate + desmethylimipramine | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + demethylimipramine N-sulfate | - |
? | |
3'-phosphoadenylylsulfate + octylamine | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + N-sulfooctylamine | - |
? | |
additional information | acceptor: primary amines, secondary amines, purified preparation also has O-sulfotransferase activities, suggesting that transfer to oxygen could represent an intrinsic function of N-sulfotransferase | Cavia porcellus | ? | - |
? | |
additional information | acceptor: primary amines, secondary amines, purified preparation also has O-sulfotransferase activities, suggesting that transfer to oxygen could represent an intrinsic function of N-sulfotransferase | Rattus norvegicus | ? | - |
? | |
additional information | acceptor: primary amines, secondary amines, purified preparation also has O-sulfotransferase activities, suggesting that transfer to oxygen could represent an intrinsic function of N-sulfotransferase | Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000 + x * 34000, SDS-PAGE | Cavia porcellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cavia porcellus |
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Cavia porcellus |
6.8 | - |
assay at | Rattus norvegicus |
6.8 | - |
assay at | Oryctolagus cuniculus |
8 | - |
assay at | Cavia porcellus |
8 | - |
assay at | Rattus norvegicus |
8 | - |
assay at | Oryctolagus cuniculus |