Information on EC 2.8.2.3 - amine sulfotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.3
-
RECOMMENDED NAME
GeneOntology No.
amine sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + an amine = adenosine 3',5'-bisphosphate + a sulfamate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:amine N-sulfotransferase
A large number of primary and secondary amines can act as acceptors, including aniline, 2-naphthylamine, cyclohexylamine and octylamine.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-08-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1,8-naphthalenediamine
adenosine 3',5'-bisphosphate + 1,8-naphthalenediamine sulfamate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + 1-naphthylamine
adenosine 3',5'-bisphosphate + 1-naphthylsulfamate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 2-naphthol
adenosine 3',5'-bisphosphate + 2-naphthyl sulfamate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 4-chloroaniline
adenosine 3',5'-bisphosphate + (4-chlorophenyl)sulfamic acid
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + an amine
adenosine 3',5'-bisphosphate + a sulfamate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + aniline
adenosine 3',5'-bisphosphate + anilinesulfamate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dopamine
adenosine 3',5'-bisphosphate + dopamine sulfamate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + p-nitrophenol
adenosine 3',5'-bisphosphate + p-nitrophenylsulfamate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 1,2,3,4-tetrahydroisoquinoline
adenosine 3',5'-bisphosphate + 1,2,3,4-tetrahydroisoquinoline N-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 1,2,3,4-tetrahydroquinoline
adenosine 3',5'-bisphosphate + 1,2,3,4-tetrahydroquinoline N-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 2-naphthylamine
adenosine 3',5'-bisphosphate + 2-naphthylsulfamate
show the reaction diagram
3'-phosphoadenylylsulfate + 3-tyramine
adenosine 3',5'-bisphosphate + 3-tyramine N-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-chloroaniline
adenosine 3',5'-bisphosphate + N-sulfo-4-chloroaniline
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 4-phenyl-1,2,3,6-tetrahydropyridine
adenosine 3',5'-bisphosphate + 4-phenyl-1,2,3,6-tetrahydropyridine N-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 4-tyramine
adenosine 3',5'-bisphosphate + 4-tyramine N-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + aniline
adenosine 3',5'-bisphosphate + N-sulfoaniline
show the reaction diagram
3'-phosphoadenylylsulfate + cyclohexylamine
adenosine 3',5'-bisphosphate + N-sulfocyclohexylamine
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + desipramine
adenosine 3',5'-bisphosphate + N-sulfo-desipramine
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + desmethylimipramine
adenosine 3',5'-bisphosphate + demethylimipramine N-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + N-sufodopamine
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + noradrenaline
adenosine 3',5'-bisphosphate + noradrenaline N-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + octylamine
adenosine 3',5'-bisphosphate + N-sulfooctylamine
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + serotonin
adenosine 3',5'-bisphosphate + serotonin N-sulfate
show the reaction diagram
-
poor substrate
-
-
?
3'-phosphoadenylylsulfate + tyramine
adenosine 3',5'-bisphosphate + tyramine N-sulfate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + an amine
adenosine 3',5'-bisphosphate + a sulfamate
show the reaction diagram
O35403
-
-
-
?
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 2-naphthylamine
adenosine 3',5'-bisphosphate + 2-naphthylsulfamate
show the reaction diagram
3'-phosphoadenylylsulfate + aniline
adenosine 3',5'-bisphosphate + N-sulfoaniline
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-Dichloro-4-nitrophenol
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5,7-dichlorokynurenic acid
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gavestinel
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i.e. 4,6-dichloro-3-[(1E)-3-oxo-3-(phenylamino)-1-propenyl]-1H-indole-2-carboxylic acid sodium salt
Kynurenic acid
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L689,560
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i.e. trans-2-carboxy-5,7-dichloro-4-phenylaminocarboxylamino-1,2,3,4-tetrahydroquinoline
L701,324
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i.e. 7-chloro-4-hydroxy-3(3-phenoxy)phenyl-2(H)-quinolinone
Mefenamic acid
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-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14
1,2,3,4-tetrahydroisoquinoline
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pH 8, 37C
38
1,2,3,4-tetrahydroquinoline
-
pH 8, 37C
0.0157 - 0.154
1-naphthol
0.2331 - 0.3026
1-Naphthylamine
0.322 - 3400
2-Naphthylamine
0.00013 - 0.149
3'-phosphoadenylylsulfate
0.382
4-phenyl-1,2,3,6-tetrahydropyridine
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.75
SULT3A1, 1-naphthylamine
7
-
two optima: pH 7.4 and pH 10, activity towards alicyclic amines is higher at pH 10
7.8 - 8
-
-
8.75
SULT3A2, 1-naphthylamine
10
-
two optima: pH 7.4 and pH 10, activity towards alicyclic amines is higher at pH 10
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
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x * 33000 + x * 34000, SDS-PAGE
34000
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x * 33000 + x * 34000, SDS-PAGE
35000
SULT3A2, SDS-PAGE
35880
calculated from nucleotide sequence
37000
SULT3A1, SDS-PAGE
60000
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gel filtration
62000
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 33000 + x * 34000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
272fold
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Ni-NTA agarose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expression of in Escherichia coli BL21; expression of in Escherichia coli BL21