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Literature summary for 2.8.2.21 extracted from

  • Yamamoto, Y.; Takahashi, I.; Ogata, N.; Nakazawa, K.
    Purification and characterization of N-acetylglucosaminyl sulfotransferase from chick corneas (2001), Arch. Biochem. Biophys., 392, 87-92.
    View publication on PubMed
Search for more literature for 2.8.2.21

Activating Compound

Activating Compound Comment Organism Structure
protamine 2fold activation at 0.01% Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
N-acetylgalactosamine slight inhibition at 25 mM Gallus gallus
N-acetylglucosamine 39% inhibition at 25 mM Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 26% stimulation at 5 mM Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE, 2 bands with 55000 Da and 66000 Da Gallus gallus
66000
-
 native PAGE Gallus gallus
66000
-
 SDS-PAGE, 2 bands with 55000 Da and 66000 Da Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gallus gallus sulfation of GlcNAc is coupled to enlongation of keratan sulfate chain ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
cornea
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000033
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + pyridylamino-GlcNAcbeta1-3Galbeta1-4GlcNAc sulfation at GlcNAc residues at the nonreducing end but no sulfation of internal GlcNAc Gallus gallus adenosine 3',5'-bisphosphate + pyridylamino-GlcNAcbeta1-3Galbeta1-4(6-sulfo)GlcNAc
-
 ?
additional information N-acetylglucosamine residues do not act as acceptors Gallus gallus ?
-
 ?
additional information sulfation of GlcNAc is coupled to enlongation of keratan sulfate chain Gallus gallus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 MES buffer Gallus gallus