Information on EC 2.8.2.21 - keratan sulfotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.21
-
RECOMMENDED NAME
GeneOntology No.
keratan sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosaminoglycan biosynthesis - keratan sulfate
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-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:keratan 6'-sulfotransferase
Sulfation takes place at the 6-position of galactosyl and N-acetylglucosaminyl residues in keratan, a proteoglycan. Not identical with EC 2.8.2.5 (chondroitin 4-sulfotransferase), EC 2.8.2.6 (choline sulfotransferase) or EC 2.8.2.17 (chondroitin 6-sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
62168-79-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
enzyme knockout mice do not exhibit a detectable deficiency in short-term lymphocyte homing
metabolism
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the enzyme is required for the generation of Siaalpha2->3[6S]beta->4[6S]GlcNAc or a closely related structure in lymph node high endothelial venules. The enzyme does does not contribute to L-selectin ligand expression or lymphocyte homing
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + N-acetyllactosamine
adenosine 3',5'-bisphosphate + 6'-sulfo N-acetyllactosamine
show the reaction diagram
-
-
-
-
-
3'-phosphoadenylylsulfate + agarose
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfotransferase I active, sulfotransferase II not
-
-
-
3'-phosphoadenylylsulfate + chitin dodecylsaccharide
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfotransferase I and II
-
-
-
3'-phosphoadenylylsulfate + fetuin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
-
3'-phosphoadenylylsulfate + GlcNAcbeta1-3Galbeta1-4GlcNACbeta1-3Galbeta1-4GlcNAcbeta1-6Manalpha1-6Manbeta-octyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
synthetic substrate, sulfation at position 6 of N-acetylgalactosamine residues, GlcNAc 6-O-sulfotransferase
-
-
-
3'-phosphoadenylylsulfate + GlyCAM-1
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
endothelial mucin-like ligand for L-selectin, products correspond to [SO3-6]GlcNAc and Galbeta1-4[SO3-6]GlcNAc
-
-
-
3'-phosphoadenylylsulfate + keratan sulfate
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + NeuAcalpha2-3Galbeta1(6-sulfo)GlcNAcbeta1-3(6-sulfo)Galbeta1-4(6-sulfo)GlcNAc
adenosine 3',5'-bisphosphate + NeuAcalpha2-3-(6-sulfo)Galbeta1-(6-sulfo)GlcNAcbeta1-3(6-sulfo)Galbeta1-4(6-sulfo)GlcNAc
show the reaction diagram
-
sulfation at position 6 of Gal residues
-
-
-
3'-phosphoadenylylsulfate + NeuAcalpha2-3Galbeta1-4GlcNAc
adenosine 3',5'-bisphosphate + NeuAcalpha2-3(6-sulfo)Galbeta1-4GlcNAc
show the reaction diagram
-
sulfation at position 6 of Gal residues, no sulfation of GlcNAc
-
-
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3'-phosphoadenylylsulfate + NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAc
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation at position 6 of Gal residues
-
-
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3'-phosphoadenylylsulfate + pyridylamino-GlcNAcbeta1-3Galbeta1-4GlcNAc
adenosine 3',5'-bisphosphate + pyridylamino-GlcNAcbeta1-3Galbeta1-4(6-sulfo)GlcNAc
show the reaction diagram
-
sulfation at GlcNAc residues at the nonreducing end but no sulfation of internal GlcNAc
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-
-
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + N-acetyllactosamine
adenosine 3',5'-bisphosphate + 6'-sulfo N-acetyllactosamine
show the reaction diagram
-
-
-
-
-
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
activates
Co2+
-
activates, optimal concentration: 5 mM
Mg2+
-
activates
Sr2+
-
activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-AMP
-
weak, above 0.08 mM
3'-AMP
-
weak, above 0.08 mM
5'-adenylylsulfate
-
-
5'-AMP
-
weak, above 0.08 mM
ADP
-
above 0.08 mM
AMP
-
above 0.08 mM
ATP
-
above 0.08 mM
cAMP
-
weak
Cu2+
-
above 1 mM, complete inhibition above 2 mM
N-acetylgalactosamine
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slight inhibition at 25 mM
N-acetylglucosamine
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39% inhibition at 25 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
protamine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00058
3'-phosphoadenylylsulfate
-
pH 6.4, 37C
0.025 - 0.38
Keratan sulfate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000013
-
-
0.000033
-
-
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
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assay at
8.5
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sulfotransferase II
8.6
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2 optima: pH 6.0 and 8.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 37
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15C: optimum, 37C: 10% sulfotransferase I activity and 14% sulfotransferase II activity of maximum
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Q9EQ0
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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expressed in high endothelial venules of Peyer's patches
Manually annotated by BRENDA team
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high expression of sulfate 6-O-sulfotransferase
Manually annotated by BRENDA team
additional information
-
tissue expression analysis of Langerin and KS6ST
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
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SDS-PAGE, 2 bands with 55000 Da and 66000 Da
66000
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native PAGE; SDS-PAGE, 2 bands with 55000 Da and 66000 Da
120000
Q9EQ0
gel filtration
140000
-
sulfotransferase II, gel filtration
220000
-
sulfotransferase I, gel filtration
240000
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
244fold
-
partial
-
sulfotransferase I and II
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
corneal enzyme
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expressed in HeLa cells
Q9EQ0
expression in CHO cells
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expression in LN464 cells
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GST-4beta
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stable expression and co-expression of Langerin gene and keratan sulfate Gal-6-sulfotransferase gene CHST1 in CHO cells, expression of fluorescent tagged Langerin, quantitative real-time PCR analysis of the KS6ST in human tissue
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is induced after brain injury
Q9EQ0
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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overexpression of KSST suppresses the expression of sialyl Lewis X on CHO cells engineered to express sialyl Lewis X, KSST expression in those cells suppresses lymphocyte rolling compared with mock-transfected CHO cells expressing 6-sulfo sialyl Lewis X, overview