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Literature summary for 2.8.2.11 extracted from

  • Eckhardt, M.; Fewou, S.N.; Ackermann, I.; Gieselmann, V.
    N-glycosylation is required for full enzymic activity of the murine galactosylceramide sulphotransferase (2002), Biochem. J., 368, 317-324.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO-GalT cells or COS cells, enzyme can be expressed in a soluble form, that is active both in vitro and in vivo Mus musculus

Protein Variants

Protein Variants Comment Organism
N312Q no activity in comparison to the wild type enzyme in vitro Mus musculus
N66Q 50% loss of activity in comparison to the wild type enzyme in vitro Mus musculus
N66Q/N312Q no activity in comparison to the wild type enzyme in vitro Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
galactocerebroside + 3'-phosphoadenylylsulfate Mus musculus enzyme catalyses the biosynthesis of sulfatides, sulfatides are important components of myelin sulfogalactoglycerolipid + adenosine 3',5'-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate the N-glycosylation of Asn-312 and Asn-66 is necessary to form a fully active enzyme Mus musculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactocerebroside + 3'-phosphoadenylylsulfate
-
 Mus musculus galactocerebroside 3-sulfate + adenosine 3',5'-bisphosphate
-
 ?
galactocerebroside + 3'-phosphoadenylylsulfate enzyme catalyses the biosynthesis of sulfatides, sulfatides are important components of myelin Mus musculus sulfogalactoglycerolipid + adenosine 3',5'-bisphosphate
-
 ?
galactosyl 1-alkyl-2-acyl-sn-glycerol + 3'-phosphoadenylylsulfate
-
 Mus musculus galactosyl 1-alkyl-2-acyl-sn-glycerol sulfate + adenosine 3',5'-bisphosphate
-
 ?