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Literature summary for 2.8.2.11 extracted from

  • Tennekoon, G.; Aitchinson, S.; Zabura, M.
    Purification and characterization of galactocerebroside sulfotransferase from rat kidney (1985), Arch. Biochem. Biophys., 240, 932-944.
    View publication on PubMed
Search for more literature for 2.8.2.11

Inhibitors

Inhibitors Comment Organism Structure
2-Deoxy-3'-phosphoadenosine 5'-phosphate
-
 Rattus norvegicus
3'-phosphoadenosine 5'-phosphate
-
 Rattus norvegicus
guanosine 3',5'-diphosphate
-
 Rattus norvegicus
Mg2+ 1-10 mM: enhances activity, 20-35 mM: inhibition Rattus norvegicus
Mn2+ 1-10 mM: enhances activity, 20-35 mM: inhibition Rattus norvegicus
additional information not: 2'-phosphoadenosine 5'-phosphate, fluorosulfonylbenzoyladenosine Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 3'-phosphoadenylylsulfate pH 6.2, 37°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1-10 mM and 20-35 mM: enhancement Rattus norvegicus
Mg2+ 1-10 mM: enhances activity, 20-35 mM: inhibition Rattus norvegicus
Mn2+ 1-10 mM: enhances activity, 20-35 mM: inhibition Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 nondenaturing PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus enzyme regulates the sulfation of glycolipids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information purified enzyme contains bound lipids, consisting primarily of cholesterol and phosphatidylcholine, removal of associated lipids results in loss of activity Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 0.1% Triton X-100, pH 7.0-7.5, stable for more than 4 weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactosylceramide + 3'-phosphoadenylylsulfate biotinylated and light-sensitive azido derivatives of lysogalactosylceramide are synthesized by the crude enzyme preparation, these derivatives remain effective substrates for the testicular enzyme Rattus norvegicus galactosylceramide sulfate + adenosine 3',5'-bisphosphate
-
 ?
additional information enzyme regulates the sulfation of glycolipids Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, complete loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.034
-
 guanosine 3',5'-diphosphate pH 6.2, 37°C Rattus norvegicus
0.3
-
 3'-phosphoadenosine 5'-phosphate pH 6.2, 37°C Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus isoelectric foccusing
-
 5.1