Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 10fold stimulation of cysteine desulfurase activity of the enzyme at 20 mM | Escherichia coli | |
L-cysteine | increases biotin production | Escherichia coli | |
pyridoxal 5'-phosphate | required for cysteine desulfurase activity of the enzyme | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C128A | reduced cysteine desulfurase activity | Escherichia coli |
C188A | cysteine desulfurase activity is reduced to 70% of the wild-type activity | Escherichia coli |
C97A | reduced cysteine desulfurase activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaBH4 | decreases cysteine desulfurase and biotin synthase activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-cysteine | pH 8, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dethiobiotin + sulfur | - |
Escherichia coli | biotin | - |
? | |
L-cysteine | enzyme displays cysteine desulfurase activity, providing it with the ability to mobilize sulfur from free cysteine | Escherichia coli | L-alanine + sulfide | - |
? |