BRENDA - Enzyme Database
show all sequences of 2.8.1.5

Isolation and characterization of thiosulfate reductases from the green alga Chlorella fusca

Schmidt, A.; Erdle, I.; Gamon, B.; Planta 162, 243-249 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
thiosulfate
above 0.5 mM
Chlorella fusca
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.156
-
thiosulfate
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR II
Chlorella fusca
0.164
-
thiosulfate
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR I
Chlorella fusca
1.54
-
dithioerythritol
pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR II
Chlorella fusca
3.1
-
dithioerythritol
pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR I
Chlorella fusca
20
-
KCN
pH 9, 37°C, with dithioerythritol as second substrate
Chlorella fusca
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
isoenzyme IIIb, gel filtration
Chlorella fusca
26500
-
isoenzyme II, gel filtration
Chlorella fusca
28000
-
isoenzyme I, gel filtration
Chlorella fusca
55000
-
isoenzyme IIIa, gel filtration
Chlorella fusca
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
thiosulfate + 2 dithioerythritol
Chlorella fusca
-
sulfite + dithioerythritol disulfide + sulfide
-
-
?
thiosulfate + 2 dithioerythritol
Chlorella fusca 211-8b
-
sulfite + dithioerythritol disulfide + sulfide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chlorella fusca
-
-
-
Chlorella fusca 211-8b
-
-
-
Purification (Commentary)
Commentary
Organism
isoenzymes: TSR I, TSR II, TSR IIIa, TSR IIIb
Chlorella fusca
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Chlorella fusca
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cyanate + dithioerythritol
activity only in presence of thiols
645575
Chlorella fusca
?
-
-
-
?
cyanate + dithioerythritol
activity only in presence of thiols
645575
Chlorella fusca 211-8b
?
-
-
-
?
additional information
little activity with: glutathione, L-cysteine, beta-mercaptoethanol
645575
Chlorella fusca
?
-
-
-
-
additional information
little activity with: glutathione, L-cysteine, beta-mercaptoethanol
645575
Chlorella fusca 211-8b
?
-
-
-
-
thiosulfate + 2 dithioerythritol
-
645575
Chlorella fusca
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + 2 dithioerythritol
-
645575
Chlorella fusca
sulfite + dithioerythritol disulfide + sulfide
-
645575
Chlorella fusca
?
thiosulfate + 2 dithioerythritol
-
645575
Chlorella fusca 211-8b
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + 2 dithioerythritol
-
645575
Chlorella fusca 211-8b
sulfite + dithioerythritol disulfide + sulfide
-
645575
Chlorella fusca 211-8b
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Chlorella fusca
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
isoenzyme TSR IIIa
Chlorella fusca
9
-
isoenzyme TSRI and TSR II
Chlorella fusca
9.5
-
isoenzyme TSR IIIb
Chlorella fusca
Cofactor
Cofactor
Commentary
Organism
Structure
thioredoxin
stimulates thiosulfate reductase IIIa and IIIb
Chlorella fusca
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thioredoxin
stimulates thiosulfate reductase IIIa and IIIb
Chlorella fusca
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
thiosulfate
above 0.5 mM
Chlorella fusca
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.156
-
thiosulfate
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR II
Chlorella fusca
0.164
-
thiosulfate
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR I
Chlorella fusca
1.54
-
dithioerythritol
pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR II
Chlorella fusca
3.1
-
dithioerythritol
pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR I
Chlorella fusca
20
-
KCN
pH 9, 37°C, with dithioerythritol as second substrate
Chlorella fusca
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
isoenzyme IIIb, gel filtration
Chlorella fusca
26500
-
isoenzyme II, gel filtration
Chlorella fusca
28000
-
isoenzyme I, gel filtration
Chlorella fusca
55000
-
isoenzyme IIIa, gel filtration
Chlorella fusca
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
thiosulfate + 2 dithioerythritol
Chlorella fusca
-
sulfite + dithioerythritol disulfide + sulfide
-
-
?
thiosulfate + 2 dithioerythritol
Chlorella fusca 211-8b
-
sulfite + dithioerythritol disulfide + sulfide
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
isoenzymes: TSR I, TSR II, TSR IIIa, TSR IIIb
Chlorella fusca
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Chlorella fusca
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cyanate + dithioerythritol
activity only in presence of thiols
645575
Chlorella fusca
?
-
-
-
?
cyanate + dithioerythritol
activity only in presence of thiols
645575
Chlorella fusca 211-8b
?
-
-
-
?
additional information
little activity with: glutathione, L-cysteine, beta-mercaptoethanol
645575
Chlorella fusca
?
-
-
-
-
additional information
little activity with: glutathione, L-cysteine, beta-mercaptoethanol
645575
Chlorella fusca 211-8b
?
-
-
-
-
thiosulfate + 2 dithioerythritol
-
645575
Chlorella fusca
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + 2 dithioerythritol
-
645575
Chlorella fusca
sulfite + dithioerythritol disulfide + sulfide
-
645575
Chlorella fusca
?
thiosulfate + 2 dithioerythritol
-
645575
Chlorella fusca 211-8b
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + 2 dithioerythritol
-
645575
Chlorella fusca 211-8b
sulfite + dithioerythritol disulfide + sulfide
-
645575
Chlorella fusca 211-8b
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Chlorella fusca
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
isoenzyme TSR IIIa
Chlorella fusca
9
-
isoenzyme TSRI and TSR II
Chlorella fusca
9.5
-
isoenzyme TSR IIIb
Chlorella fusca
Other publictions for EC 2.8.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742170
Wang
Identification and characteri ...
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993-999
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-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
1
-
2
1
1
-
2
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
-
2
1
1
-
2
1
-
-
-
-
-
-
725273
Stoffels
Thiosulfate reduction in Salmo ...
Salmonella enterica
J. Bacteriol.
194
475-485
2012
-
-
1
-
-
-
3
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645578
Bang
-
Cadmium, lead, and zinc remova ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biotechnol. Lett.
22
1331-1335
2000
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645577
Bagchi
-
Partial purification and regul ...
Phormidium uncinatum
Indian J. Exp. Biol.
35
876-880
1997
-
-
-
-
-
-
-
1
-
-
-
2
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645579
Sugio
-
Thiosulfate reductase from a m ...
thermophilic iron-oxidizing bacterium strain TI-1
Biosci. Biotechnol. Biochem.
61
470-474
1997
3
-
-
-
-
-
4
3
1
-
2
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
1
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
4
-
3
1
-
2
-
-
-
-
1
-
-
-
-
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
645576
Akagi
-
Thiosulfate and trithionate re ...
Desulfotomaculum nigrificans, Desulfovibrio vulgaris
Methods Enzymol.
243
260-270
1994
-
-
-
-
-
2
5
-
-
-
1
-
-
2
1
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
2
-
5
-
-
-
-
1
-
-
1
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
645575
Schmidt
Isolation and characterization ...
Chlorella fusca 211-8b, Chlorella fusca
Planta
162
243-249
1984
-
-
-
-
-
-
1
5
-
-
4
2
-
2
-
-
1
-
-
-
1
-
8
-
1
-
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
-
-
4
2
-
-
-
1
-
-
1
-
8
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-