We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme from Chlorella shows very little activity towards monothiols such as glutathione and cysteine (cf. EC 2.8.1.3 thiosulfate---thiolsulfurtransferase). The enzyme probably transfers the sulfur atom onto one thiol group to form -S-S-, and sulfide is spontaneously expelled from this by reaction with the other thiol group. May be identical with EC 2.8.1.1 thiosulfate sulfurtransferase.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
reductase, thiosulfate, Sft, thiosulfate reductase, TSR,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
reductase, thiosulfate
-
-
-
thiosulfate reductase
-
-
-
thiosulfate reductase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thiosulfate + dithioerythritol = sulfite + 4,5-cis-dihydroxy-1,2-dithiacyclohexane (i.e. oxidized dithioerythritol) + sulfide
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
sulfur atom transfer
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thiosulfate:dithioerythritol sulfurtransferase
The enzyme from Chlorella shows very little activity towards monothiols such as glutathione and cysteine (cf. EC 2.8.1.3 thiosulfate---thiolsulfurtransferase). The enzyme probably transfers the sulfur atom onto one thiol group to form -S-S-, and sulfide is spontaneously expelled from this by reaction with the other thiol group. May be identical with EC 2.8.1.1 thiosulfate sulfurtransferase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cyanate + dithioerythritol
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
thiosulfate + 2 dithiothreitol
sulfite + dithiothreitol disulfide + sulfide
-
-
-
?
thiosulfate + dithiothreitol
sulfite + oxidized dithiothreitol + sulfide
-
-
-
-
?
thiosulfate + menaquinol
sulfite + oxidized menaquinol + sulfide
-
-
-
-
?
thiosulfate + methylviologen
sulfide + ?
thiosulfate + NAD(P)H
H2S + NAD(P)+ + sulfite
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
?
thiosulfate + NADH
H2S + NAD+ + sulfite
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
-
?
additional information
?
-
cyanate + dithioerythritol
?
-
activity only in presence of thiols
-
-
?
cyanate + dithioerythritol
?
-
activity only in presence of thiols
-
-
?
KCN + dithioerythritol
?
-
-
-
-
?
KCN + dithioerythritol
?
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + methylviologen
sulfide + ?
-
-
-
-
?
thiosulfate + methylviologen
sulfide + ?
-
-
-
-
?
additional information
?
-
enzyme additionally hydrolyzes ATP
-
-
?
additional information
?
-
-
ubiquinone is not an electron donor to thiosulfate reductase
-
-
?
additional information
?
-
-
enzyme also precipitates metals such as zinc, lead and cadmium as metal sulfide complexes
-
-
?
additional information
?
-
-
little activity with: glutathione, L-cysteine, beta-mercaptoethanol
-
-
?
additional information
?
-
-
little activity with: glutathione, L-cysteine, beta-mercaptoethanol
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FAD
-
enzyme contains FAD as prosthetic group
thioredoxin
-
stimulates thiosulfate reductase IIIa and IIIb
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3,3',4',5-tetrachlorosalicylanilide
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of 3,3',4',5-tetrachlorosalicylanilide is added
4-chloromercuribenzene sufonic acid
thermophilic iron-oxidizing bacterium strain TI-1
-
strong inhibition at 0.1 mM
carbonyl cyanide-3-chlorophenylhydrazone
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of the protonophore carbonyl cyanide-3-chlorophenylhydrazone is added
carbonyl cyanide-4-trifluoromethoxyphenylhydrazone
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of carbonyl cyanide-4-trifluoromethoxyphenylhydrazone is added
Co2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
Ni2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
thiosulfate
-
above 0.5 mM
Zn2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EDTA
thermophilic iron-oxidizing bacterium strain TI-1
-
-
o-phenanthroline
thermophilic iron-oxidizing bacterium strain TI-1
-
-
Sodium cyanide
thermophilic iron-oxidizing bacterium strain TI-1
-
2.5fold increase at 1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.54 - 3.1
dithioerythritol
1
dithiothreitol
pH 8.0, 30°C
20
KCN
-
pH 9, 37°C, with dithioerythritol as second substrate
0.125
NADH
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
5
NADPH
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
1.54
dithioerythritol
-
pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR II
3.1
dithioerythritol
-
pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR I
0.1
thiosulfate
-
with dithioerythritol as second substrate
0.156
thiosulfate
-
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR II
0.164
thiosulfate
-
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR I
0.29
thiosulfate
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5
dithiothreitol
pH 8.0, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6
thermophilic iron-oxidizing bacterium strain TI-1
-
-
9
-
isoenzyme TSRI and TSR II
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
60
thermophilic iron-oxidizing bacterium strain TI-1
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thermophilic iron-oxidizing bacterium strain TI-1
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
A0A073KXE8_9GAMM
767
0
84024
TrEMBL
-
B8F982_DESAL
695
0
77271
TrEMBL
-
E1SPL6_FERBD
Ferrimonas balearica (strain DSM 9799 / CCM 4581 / KCTC 23876 / PAT)
190
0
20986
TrEMBL
-
I3XUI8_SULBS
Sulfurospirillum barnesii (strain ATCC 700032 / DSM 10660 / SES-3)
189
0
21308
TrEMBL
-
I3XUI7_SULBS
Sulfurospirillum barnesii (strain ATCC 700032 / DSM 10660 / SES-3)
757
0
84489
TrEMBL
-
M1P6T7_DESSD
Desulfocapsa sulfexigens (strain DSM 10523 / SB164P1)
704
0
79562
TrEMBL
-
E1SPL5_FERBD
Ferrimonas balearica (strain DSM 9799 / CCM 4581 / KCTC 23876 / PAT)
759
0
82517
TrEMBL
-
K4LE72_THEPS
Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 12270 / PB)
728
0
81569
TrEMBL
-
L0FBP2_DESDL
Desulfitobacterium dichloroeliminans (strain LMG P-21439 / DCA1)
190
0
20626
TrEMBL
-
K4LBV6_THEPS
Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 12270 / PB)
732
0
82512
TrEMBL
-
M5PP39_DESAF
692
0
76860
TrEMBL
-
A0A0H3AAY6_DESVV
Desulfovibrio vulgaris subsp. vulgaris (strain DP4)
698
0
76282
TrEMBL
-
I3RJK8_PHACH
181
0
20723
TrEMBL
Mitochondrion (Reliability: 1 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
16300
-
sedimentation equilibrium experiments
24000
-
isoenzyme IIIb, gel filtration
243000
thermophilic iron-oxidizing bacterium strain TI-1
-
gel filtration
26500
-
isoenzyme II, gel filtration
28000
-
isoenzyme I, gel filtration
55000
-
isoenzyme IIIa, gel filtration
58000
thermophilic iron-oxidizing bacterium strain TI-1
-
homotetramer, 4 * 58000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 20680, calculated, x * 28000, SDS-PAGE
tetramer
thermophilic iron-oxidizing bacterium strain TI-1
-
homotetramer, 4 * 58000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
C137A
complete loss of both sulfurtransferase and ATPase activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.5
50 min, 68% residual activity
742170
8.5
50 min, 56% residual activity
742170
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
100
thermophilic iron-oxidizing bacterium strain TI-1
-
no enzyme activity after 15 min
30
50 min, 68% residual activity
40
50 min, 51% residual activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
-
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
sensitive to oxygen
-
645576
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
isoenzymes: TSR I, TSR II, TSR IIIa, TSR IIIb
-
-
-
-
thermophilic iron-oxidizing bacterium strain TI-1
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli DH5alpha cells
-
expression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Schmidt, A.; Erdle, I.; Gamon, B.
Isolation and characterization of thiosulfate reductases from the green alga Chlorella fusca
Planta
162
243-249
1984
[Chlorella] fusca, [Chlorella] fusca 211-8b
brenda
Akagi, J.M.; Drake, H.L.; Kim, J.H.; Gevertz, D.
Thiosulfate and trithionate reductases
Methods Enzymol.
243
260-270
1994
Desulfotomaculum nigrificans, Desulfovibrio vulgaris
-
brenda
Bagchi, D.; Verma, D.
Partial purification and regulation of sulfur metabolizing enzymes in a cyanobacterium Phormidium uncinatum
Indian J. Exp. Biol.
35
876-880
1997
Phormidium uncinatum
-
brenda
Bang, S.W.; Clark, D.S.; Keasling, J.D.
Cadmium, lead, and zinc removal by expression of the thiosulfate reductase gene from Salmonella typhimurium in Escherichia coli
Biotechnol. Lett.
22
1331-1335
2000
Salmonella enterica subsp. enterica serovar Typhimurium
-
brenda
Sugio, T.; Kishimoto, K.; Oda, K.
Thiosulfate reductase from a moderately thermophilic iron-oxidizing bacterium, strain TI-1: purification and characterization
Biosci. Biotechnol. Biochem.
61
470-474
1997
thermophilic iron-oxidizing bacterium strain TI-1
-
brenda
Stoffels, L.; Krehenbrink, M.; Berks, B.C.; Unden, G.
Thiosulfate reduction in Salmonella enterica is driven by the proton motive force
J. Bacteriol.
194
475-485
2012
Salmonella enterica
brenda
Wang, Z.; Wang, G.; Xiang, Q.; Zhang, Y.; Wang, H.
Identification and characterization of a multi-domain sulfurtransferase in Phanerochaete chrysosporium
Biotechnol. Lett.
36
993-999
2014
Phanerochaete chrysosporium (I3RJK8)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
2.8.1.5 thiosulfate-dithiol sulfurtransferase
more
top
Information
