Information on EC 2.8.1.5 - thiosulfate-dithiol sulfurtransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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EC NUMBER
COMMENTARY hide
2.8.1.5
-
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RECOMMENDED NAME
GeneOntology No.
thiosulfate-dithiol sulfurtransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thiosulfate + dithioerythritol = sulfite + 4,5-cis-dihydroxy-1,2-dithiacyclohexane (i.e. oxidized dithioerythritol) + sulfide
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfur atom transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sulfur metabolism
-
-
Microbial metabolism in diverse environments
-
-
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SYSTEMATIC NAME
IUBMB Comments
thiosulfate:dithioerythritol sulfurtransferase
The enzyme from Chlorella shows very little activity towards monothiols such as glutathione and cysteine (cf. EC 2.8.1.3 thiosulfate---thiolsulfurtransferase). The enzyme probably transfers the sulfur atom onto one thiol group to form -S-S-, and sulfide is spontaneously expelled from this by reaction with the other thiol group. May be identical with EC 2.8.1.1 thiosulfate sulfurtransferase.
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CAS REGISTRY NUMBER
COMMENTARY hide
9059-49-8
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cyanate + dithioerythritol
?
show the reaction diagram
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
show the reaction diagram
thiosulfate + 2 dithiothreitol
sulfite + dithiothreitol disulfide + sulfide
show the reaction diagram
-
-
-
?
thiosulfate + dithiothreitol
sulfite + oxidized dithiothreitol + sulfide
show the reaction diagram
-
-
-
-
?
thiosulfate + menaquinol
sulfite + oxidized menaquinol + sulfide
show the reaction diagram
-
-
-
-
?
thiosulfate + methylviologen
sulfide + ?
show the reaction diagram
thiosulfate + NAD(P)H
H2S + NAD(P)+ + sulfite
show the reaction diagram
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
?
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
enzyme contains FAD as prosthetic group
thioredoxin
-
stimulates thiosulfate reductase IIIa and IIIb
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-bipyridine
-
-
3,3',4',5-tetrachlorosalicylanilide
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of 3,3',4',5-tetrachlorosalicylanilide is added
4-chloromercuribenzene sufonic acid
thermophilic iron-oxidizing bacterium strain TI-1
-
strong inhibition at 0.1 mM
carbonyl cyanide-3-chlorophenylhydrazone
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of the protonophore carbonyl cyanide-3-chlorophenylhydrazone is added
carbonyl cyanide-4-trifluoromethoxyphenylhydrazone
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of carbonyl cyanide-4-trifluoromethoxyphenylhydrazone is added
Co2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
Ni2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
o-phenanthroline
-
-
Sulfhydryl agents
-
-
-
thiosulfate
-
above 0.5 mM
trithionate
-
-
Zn2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
thermophilic iron-oxidizing bacterium strain TI-1
-
-
o-phenanthroline
thermophilic iron-oxidizing bacterium strain TI-1
-
-
Sodium cyanide
thermophilic iron-oxidizing bacterium strain TI-1
-
2.5fold increase at 1 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.54 - 3.1
dithioerythritol
1
dithiothreitol
pH 8.0, 30°C
20
KCN
-
pH 9, 37°C, with dithioerythritol as second substrate
0.125
NADH
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
5
NADPH
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
0.1 - 0.29
thiosulfate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
dithiothreitol
pH 8.0, 30°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
thermophilic iron-oxidizing bacterium strain TI-1
-
-
8.5
-
isoenzyme TSR IIIa
9
-
isoenzyme TSRI and TSR II
9.5
-
isoenzyme TSR IIIb
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
60
thermophilic iron-oxidizing bacterium strain TI-1
-
-
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
thermophilic iron-oxidizing bacterium strain TI-1
-
-
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16300
-
sedimentation equilibrium experiments
24000
-
isoenzyme IIIb, gel filtration
26500
-
isoenzyme II, gel filtration
28000
-
isoenzyme I, gel filtration
55000
-
isoenzyme IIIa, gel filtration
58000
thermophilic iron-oxidizing bacterium strain TI-1
-
homotetramer, 4 * 58000, SDS-PAGE
243000
thermophilic iron-oxidizing bacterium strain TI-1
-
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 20680, calculated, x * 28000, SDS-PAGE
tetramer
thermophilic iron-oxidizing bacterium strain TI-1
-
homotetramer, 4 * 58000, SDS-PAGE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
50 min, 68% residual activity
742170
8.5
50 min, 56% residual activity
742170
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
50 min, 68% residual activity
40
50 min, 51% residual activity
100
thermophilic iron-oxidizing bacterium strain TI-1
-
no enzyme activity after 15 min
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
sensitive to oxygen
-
645576
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
isoenzymes: TSR I, TSR II, TSR IIIa, TSR IIIb
-
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha cells
-
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C137A
complete loss of both sulfurtransferase and ATPase activity
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LINKS TO OTHER DATABASES (specific for EC-Number 2.8.1.5)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)