Information on EC 2.8.1.5 - thiosulfate-dithiol sulfurtransferase

for references in articles please use BRENDA:EC2.8.1.5
Word Map on EC 2.8.1.5
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.8.1.5
-
RECOMMENDED NAME
GeneOntology No.
thiosulfate-dithiol sulfurtransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thiosulfate + dithioerythritol = sulfite + 4,5-cis-dihydroxy-1,2-dithiacyclohexane (i.e. oxidized dithioerythritol) + sulfide
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfur atom transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sulfur metabolism
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
thiosulfate:dithioerythritol sulfurtransferase
The enzyme from Chlorella shows very little activity towards monothiols such as glutathione and cysteine (cf. EC 2.8.1.3 thiosulfate---thiolsulfurtransferase). The enzyme probably transfers the sulfur atom onto one thiol group to form -S-S-, and sulfide is spontaneously expelled from this by reaction with the other thiol group. May be identical with EC 2.8.1.1 thiosulfate sulfurtransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
9059-49-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cyanate + dithioerythritol
?
show the reaction diagram
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
show the reaction diagram
thiosulfate + 2 dithiothreitol
sulfite + dithiothreitol disulfide + sulfide
show the reaction diagram
-
-
-
?
thiosulfate + dithiothreitol
sulfite + oxidized dithiothreitol + sulfide
show the reaction diagram
-
-
-
-
?
thiosulfate + menaquinol
sulfite + oxidized menaquinol + sulfide
show the reaction diagram
-
-
-
-
?
thiosulfate + methylviologen
sulfide + ?
show the reaction diagram
thiosulfate + NAD(P)H
H2S + NAD(P)+ + sulfite
show the reaction diagram
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
enzyme contains FAD as prosthetic group
thioredoxin
-
stimulates thiosulfate reductase IIIa and IIIb
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-bipyridine
-
-
3,3',4',5-tetrachlorosalicylanilide
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of 3,3',4',5-tetrachlorosalicylanilide is added
4-chloromercuribenzene sufonic acid
thermophilic iron-oxidizing bacterium strain TI-1
-
strong inhibition at 0.1 mM
carbonyl cyanide-3-chlorophenylhydrazone
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of the protonophore carbonyl cyanide-3-chlorophenylhydrazone is added
carbonyl cyanide-4-trifluoromethoxyphenylhydrazone
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of carbonyl cyanide-4-trifluoromethoxyphenylhydrazone is added
Co2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
Ni2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
o-phenanthroline
-
-
Sulfhydryl agents
-
-
-
thiosulfate
-
above 0.5 mM
trithionate
-
-
Zn2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
thermophilic iron-oxidizing bacterium strain TI-1
-
-
o-phenanthroline
thermophilic iron-oxidizing bacterium strain TI-1
-
-
Sodium cyanide
thermophilic iron-oxidizing bacterium strain TI-1
-
2.5fold increase at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.54 - 3.1
dithioerythritol
1
dithiothreitol
pH 8.0, 30°C
20
KCN
-
pH 9, 37°C, with dithioerythritol as second substrate
0.125
NADH
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
5
NADPH
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
0.1 - 0.29
thiosulfate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
dithiothreitol
pH 8.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
thermophilic iron-oxidizing bacterium strain TI-1
-
-
8.5
-
isoenzyme TSR IIIa
9
-
isoenzyme TSRI and TSR II
9.5
-
isoenzyme TSR IIIb
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
60
thermophilic iron-oxidizing bacterium strain TI-1
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
thermophilic iron-oxidizing bacterium strain TI-1
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16300
-
sedimentation equilibrium experiments
24000
-
isoenzyme IIIb, gel filtration
26500
-
isoenzyme II, gel filtration
28000
-
isoenzyme I, gel filtration
55000
-
isoenzyme IIIa, gel filtration
58000
thermophilic iron-oxidizing bacterium strain TI-1
-
homotetramer, 4 * 58000, SDS-PAGE
243000
thermophilic iron-oxidizing bacterium strain TI-1
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 20680, calculated, x * 28000, SDS-PAGE
tetramer
thermophilic iron-oxidizing bacterium strain TI-1
-
homotetramer, 4 * 58000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
50 min, 68% residual activity
742170
8.5
50 min, 56% residual activity
742170
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
50 min, 68% residual activity
40
50 min, 51% residual activity
100
thermophilic iron-oxidizing bacterium strain TI-1
-
no enzyme activity after 15 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
sensitive to oxygen
-
645576
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
isoenzymes: TSR I, TSR II, TSR IIIa, TSR IIIb
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha cells
-
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C137A
complete loss of both sulfurtransferase and ATPase activity