We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.8.1.5 - thiosulfate-dithiol sulfurtransferase for references in articles please use BRENDA:EC2.8.1.5Word Map on EC 2.8.1.5
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thiosulfate-dithiol sulfurtransferase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thiosulfate + dithioerythritol = sulfite + 4,5-cis-dihydroxy-1,2-dithiacyclohexane (i.e. oxidized dithioerythritol) + sulfide
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
sulfur atom transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Microbial metabolism in diverse environments
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thiosulfate:dithioerythritol sulfurtransferase
The enzyme from Chlorella shows very little activity towards monothiols such as glutathione and cysteine (cf. EC 2.8.1.3 thiosulfate---thiolsulfurtransferase). The enzyme probably transfers the sulfur atom onto one thiol group to form -S-S-, and sulfide is spontaneously expelled from this by reaction with the other thiol group. May be identical with EC 2.8.1.1 thiosulfate sulfurtransferase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
reductase, thiosulfate
-
-
-
-
thiosulfate reductase
-
-
-
-
thiosulfate reductase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cyanate + dithioerythritol
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
thiosulfate + 2 dithiothreitol
sulfite + dithiothreitol disulfide + sulfide
-
-
-
?
thiosulfate + dithiothreitol
sulfite + oxidized dithiothreitol + sulfide
-
-
-
-
?
thiosulfate + menaquinol
sulfite + oxidized menaquinol + sulfide
-
-
-
-
?
thiosulfate + methylviologen
sulfide + ?
thiosulfate + NAD(P)H
H2S + NAD(P)+ + sulfite
thermophilic iron-oxidizing bacterium strain TI-1
-
-
-
?
additional information
?
-
cyanate + dithioerythritol
?
-
activity only in presence of thiols
-
-
?
cyanate + dithioerythritol
?
-
activity only in presence of thiols
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + methylviologen
sulfide + ?
-
-
-
-
?
thiosulfate + methylviologen
sulfide + ?
-
-
-
-
?
additional information
?
-
-
little activity with: glutathione, L-cysteine, beta-mercaptoethanol
-
-
-
additional information
?
-
-
little activity with: glutathione, L-cysteine, beta-mercaptoethanol
-
-
-
additional information
?
-
enzyme additionally hydrolyzes ATP
-
-
-
additional information
?
-
-
ubiquinone is not an electron donor to thiosulfate reductase
-
-
-
additional information
?
-
-
enzyme also precipitates metals such as zinc, lead and cadmium as metal sulfide complexes
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FAD
-
enzyme contains FAD as prosthetic group
thioredoxin
-
stimulates thiosulfate reductase IIIa and IIIb
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3,3',4',5-tetrachlorosalicylanilide
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of 3,3',4',5-tetrachlorosalicylanilide is added
4-chloromercuribenzene sufonic acid
thermophilic iron-oxidizing bacterium strain TI-1
-
strong inhibition at 0.1 mM
carbonyl cyanide-3-chlorophenylhydrazone
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of the protonophore carbonyl cyanide-3-chlorophenylhydrazone is added
carbonyl cyanide-4-trifluoromethoxyphenylhydrazone
-
sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of carbonyl cyanide-4-trifluoromethoxyphenylhydrazone is added
Co2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
Ni2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
thiosulfate
-
above 0.5 mM
Zn2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EDTA
thermophilic iron-oxidizing bacterium strain TI-1
-
-
o-phenanthroline
thermophilic iron-oxidizing bacterium strain TI-1
-
-
Sodium cyanide
thermophilic iron-oxidizing bacterium strain TI-1
-
2.5fold increase at 1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.54 - 3.1
dithioerythritol
1
dithiothreitol
pH 8.0, 30°C
20
KCN
-
pH 9, 37°C, with dithioerythritol as second substrate
0.125
NADH
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
5
NADPH
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
1.54
dithioerythritol
-
pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR II
3.1
dithioerythritol
-
pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR I
0.1
thiosulfate
-
with dithioerythritol as second substrate
0.156
thiosulfate
-
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR II
0.164
thiosulfate
-
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR I
0.29
thiosulfate
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5
dithiothreitol
pH 8.0, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6
thermophilic iron-oxidizing bacterium strain TI-1
-
-
9
-
isoenzyme TSRI and TSR II
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
60
thermophilic iron-oxidizing bacterium strain TI-1
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
thermophilic iron-oxidizing bacterium strain TI-1
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
16300
-
sedimentation equilibrium experiments
24000
-
isoenzyme IIIb, gel filtration
26500
-
isoenzyme II, gel filtration
28000
-
isoenzyme I, gel filtration
55000
-
isoenzyme IIIa, gel filtration
58000
thermophilic iron-oxidizing bacterium strain TI-1
-
homotetramer, 4 * 58000, SDS-PAGE
243000
thermophilic iron-oxidizing bacterium strain TI-1
-
gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 20680, calculated, x * 28000, SDS-PAGE
tetramer
thermophilic iron-oxidizing bacterium strain TI-1
-
homotetramer, 4 * 58000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.5
50 min, 68% residual activity
742170
8.5
50 min, 56% residual activity
742170
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30
50 min, 68% residual activity
40
50 min, 51% residual activity
100
thermophilic iron-oxidizing bacterium strain TI-1
-
no enzyme activity after 15 min
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
-
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
sensitive to oxygen
-
645576
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
isoenzymes: TSR I, TSR II, TSR IIIa, TSR IIIb
-
-
-
-
thermophilic iron-oxidizing bacterium strain TI-1
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli DH5alpha cells
-
expression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
C137A
complete loss of both sulfurtransferase and ATPase activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
I3XUI8_SULBS
Sulfurospirillum barnesii (strain ATCC 700032 / DSM 10660 / SES-3)
189
21308
TrEMBL
E1SPL6_FERBD
Ferrimonas balearica (strain DSM 9799 / CCM 4581 / PAT)
190
20986
TrEMBL
M5PP39_DESAF
692
76860
TrEMBL
M1P6T7_DESSD
Desulfocapsa sulfexigens (strain DSM 10523 / SB164P1)
704
79562
TrEMBL
L0FBP2_DESDL
Desulfitobacterium dichloroeliminans (strain LMG P-21439 / DCA1)
190
20626
TrEMBL
K4LE72_THEPS
Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 12270 / PB)
728
81569
TrEMBL
K4LBV6_THEPS
Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 12270 / PB)
732
82512
TrEMBL
E1SPL5_FERBD
Ferrimonas balearica (strain DSM 9799 / CCM 4581 / PAT)
759
82517
TrEMBL
A0A0H3AAY6_DESVV
Desulfovibrio vulgaris subsp. vulgaris (strain DP4)
698
76282
TrEMBL
I3RJK8_PHACH
181
20723
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Schmidt, A.; Erdle, I.; Gamon, B.
Isolation and characterization of thiosulfate reductases from the green alga Chlorella fusca
Planta
162
243-249
1984
Chlorella fusca 211-8b, Chlorella fusca
brenda
Akagi, J.M.; Drake, H.L.; Kim, J.H.; Gevertz, D.
Thiosulfate and trithionate reductases
Methods Enzymol.
243
260-270
1994
Desulfotomaculum nigrificans, Desulfovibrio vulgaris
-
brenda
Bagchi, D.; Verma, D.
Partial purification and regulation of sulfur metabolizing enzymes in a cyanobacterium Phormidium uncinatum
Indian J. Exp. Biol.
35
876-880
1997
Phormidium uncinatum
-
brenda
Bang, S.W.; Clark, D.S.; Keasling, J.D.
Cadmium, lead, and zinc removal by expression of the thiosulfate reductase gene from Salmonella typhimurium in Escherichia coli
Biotechnol. Lett.
22
1331-1335
2000
Salmonella enterica subsp. enterica serovar Typhimurium
-
brenda
Sugio, T.; Kishimoto, K.; Oda, K.
Thiosulfate reductase from a moderately thermophilic iron-oxidizing bacterium, strain TI-1: purification and characterization
Biosci. Biotechnol. Biochem.
61
470-474
1997
thermophilic iron-oxidizing bacterium strain TI-1
-
brenda
Stoffels, L.; Krehenbrink, M.; Berks, B.C.; Unden, G.
Thiosulfate reduction in Salmonella enterica is driven by the proton motive force
J. Bacteriol.
194
475-485
2012
Salmonella enterica
brenda
Wang, Z.; Wang, G.; Xiang, Q.; Zhang, Y.; Wang, H.
Identification and characterization of a multi-domain sulfurtransferase in Phanerochaete chrysosporium
Biotechnol. Lett.
36
993-999
2014
Phanerochaete chrysosporium (I3RJK8)
brenda
html completed