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Information on EC 2.8.1.5 - thiosulfate-dithiol sulfurtransferase for references in articles please use BRENDA:EC2.8.1.5
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EC Tree
IUBMB Comments The enzyme from Chlorella shows very little activity towards monothiols such as glutathione and cysteine (cf. EC 2.8.1.3 thiosulfate---thiolsulfurtransferase). The enzyme probably transfers the sulfur atom onto one thiol group to form -S-S-, and sulfide is spontaneously expelled from this by reaction with the other thiol group. May be identical with EC 2.8.1.1 thiosulfate sulfurtransferase.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
reductase, thiosulfate, Sft, thiosulfate reductase, TSR,
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reductase, thiosulfate
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thiosulfate reductase
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thiosulfate reductase
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-
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thiosulfate + dithioerythritol = sulfite + 4,5-cis-dihydroxy-1,2-dithiacyclohexane (i.e. oxidized dithioerythritol) + sulfide
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-
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sulfur atom transfer
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-
-
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thiosulfate:dithioerythritol sulfurtransferase
The enzyme from Chlorella shows very little activity towards monothiols such as glutathione and cysteine (cf. EC 2.8.1.3 thiosulfate---thiolsulfurtransferase). The enzyme probably transfers the sulfur atom onto one thiol group to form -S-S-, and sulfide is spontaneously expelled from this by reaction with the other thiol group. May be identical with EC 2.8.1.1 thiosulfate sulfurtransferase.
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cyanate + dithioerythritol
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
thiosulfate + 2 dithiothreitol
sulfite + dithiothreitol disulfide + sulfide
-
-
-
?
thiosulfate + dithiothreitol
sulfite + oxidized dithiothreitol + sulfide
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-
-
-
?
thiosulfate + menaquinol
sulfite + oxidized menaquinol + sulfide
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-
-
-
?
thiosulfate + methylviologen
sulfide + ?
thiosulfate + NAD(P)H
H2S + NAD(P)+ + sulfite
thermophilic iron-oxidizing bacterium strain TI-1
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-
-
?
additional information
?
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cyanate + dithioerythritol
?
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activity only in presence of thiols
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-
?
cyanate + dithioerythritol
?
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activity only in presence of thiols
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-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
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-
-
-
?
thiosulfate + methylviologen
sulfide + ?
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-
-
-
?
thiosulfate + methylviologen
sulfide + ?
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-
-
-
?
additional information
?
-
-
little activity with: glutathione, L-cysteine, beta-mercaptoethanol
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-
additional information
?
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little activity with: glutathione, L-cysteine, beta-mercaptoethanol
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-
additional information
?
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enzyme additionally hydrolyzes ATP
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-
additional information
?
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ubiquinone is not an electron donor to thiosulfate reductase
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additional information
?
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enzyme also precipitates metals such as zinc, lead and cadmium as metal sulfide complexes
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-
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thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
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-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
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-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
thiosulfate + 2 dithioerythritol
sulfite + dithioerythritol disulfide + sulfide
-
-
-
-
?
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FAD
-
enzyme contains FAD as prosthetic group
thioredoxin
-
stimulates thiosulfate reductase IIIa and IIIb
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3,3',4',5-tetrachlorosalicylanilide
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sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of 3,3',4',5-tetrachlorosalicylanilide is added
4-chloromercuribenzene sufonic acid
thermophilic iron-oxidizing bacterium strain TI-1
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strong inhibition at 0.1 mM
carbonyl cyanide-3-chlorophenylhydrazone
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sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of the protonophore carbonyl cyanide-3-chlorophenylhydrazone is added
carbonyl cyanide-4-trifluoromethoxyphenylhydrazone
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sulfide production is nearly completely inhibited (less than 5% residual activity) when low concentrations of carbonyl cyanide-4-trifluoromethoxyphenylhydrazone is added
Co2+
thermophilic iron-oxidizing bacterium strain TI-1
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slight inhibition at 1 mM
Ni2+
thermophilic iron-oxidizing bacterium strain TI-1
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slight inhibition at 1 mM
thiosulfate
-
above 0.5 mM
Zn2+
thermophilic iron-oxidizing bacterium strain TI-1
-
slight inhibition at 1 mM
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EDTA
thermophilic iron-oxidizing bacterium strain TI-1
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-
o-phenanthroline
thermophilic iron-oxidizing bacterium strain TI-1
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Sodium cyanide
thermophilic iron-oxidizing bacterium strain TI-1
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2.5fold increase at 1 mM
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1.54 - 3.1
dithioerythritol
1
dithiothreitol
pH 8.0, 30°C
20
KCN
-
pH 9, 37°C, with dithioerythritol as second substrate
0.125
NADH
thermophilic iron-oxidizing bacterium strain TI-1
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pH 6, 45°C
5
NADPH
thermophilic iron-oxidizing bacterium strain TI-1
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pH 6, 45°C
1.54
dithioerythritol
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pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR II
3.1
dithioerythritol
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pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR I
0.1
thiosulfate
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with dithioerythritol as second substrate
0.156
thiosulfate
-
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR II
0.164
thiosulfate
-
pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR I
0.29
thiosulfate
thermophilic iron-oxidizing bacterium strain TI-1
-
pH 6, 45°C
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5
dithiothreitol
pH 8.0, 30°C
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additional information
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-
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6
thermophilic iron-oxidizing bacterium strain TI-1
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9
-
isoenzyme TSRI and TSR II
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60
thermophilic iron-oxidizing bacterium strain TI-1
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-
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UniProt
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thermophilic iron-oxidizing bacterium strain TI-1
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brenda
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thermophilic iron-oxidizing bacterium strain TI-1
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I3RJK8_PHACH
181
0
20723
TrEMBL
L0FBP2_DESDL
Desulfitobacterium dichloroeliminans (strain LMG P-21439 / DCA1)
190
0
20626
TrEMBL
I3XUI8_SULBS
Sulfurospirillum barnesii (strain ATCC 700032 / DSM 10660 / SES-3)
189
0
21308
TrEMBL
M5PP39_DESAF
692
0
76860
TrEMBL
E1SPL6_FERBD
Ferrimonas balearica (strain DSM 9799 / CCM 4581 / PAT)
190
0
20986
TrEMBL
A0A073KXE8_9GAMM
767
0
84024
TrEMBL
M1P6T7_DESSD
Desulfocapsa sulfexigens (strain DSM 10523 / SB164P1)
704
0
79562
TrEMBL
E1SPL5_FERBD
Ferrimonas balearica (strain DSM 9799 / CCM 4581 / PAT)
759
0
82517
TrEMBL
A0A0H3AAY6_DESVV
Desulfovibrio vulgaris subsp. vulgaris (strain DP4)
698
0
76282
TrEMBL
B8F982_DESAL
695
0
77271
TrEMBL
K4LBV6_THEPS
Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 12270 / PB)
732
0
82512
TrEMBL
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16300
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sedimentation equilibrium experiments
24000
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isoenzyme IIIb, gel filtration
26500
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isoenzyme II, gel filtration
28000
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isoenzyme I, gel filtration
55000
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isoenzyme IIIa, gel filtration
58000
thermophilic iron-oxidizing bacterium strain TI-1
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homotetramer, 4 * 58000, SDS-PAGE
243000
thermophilic iron-oxidizing bacterium strain TI-1
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gel filtration
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?
x * 20680, calculated, x * 28000, SDS-PAGE
tetramer
thermophilic iron-oxidizing bacterium strain TI-1
-
homotetramer, 4 * 58000, SDS-PAGE
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C137A
complete loss of both sulfurtransferase and ATPase activity
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7.5
50 min, 68% residual activity
742170
8.5
50 min, 56% residual activity
742170
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30
50 min, 68% residual activity
40
50 min, 51% residual activity
100
thermophilic iron-oxidizing bacterium strain TI-1
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no enzyme activity after 15 min
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enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
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enzyme is more stable in phosphate buffer than in Tris, borate or triethanolamine buffer
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sensitive to oxygen
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645576
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isoenzymes: TSR I, TSR II, TSR IIIa, TSR IIIb
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thermophilic iron-oxidizing bacterium strain TI-1
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expressed in Escherichia coli DH5alpha cells
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expression in Escherichia coli
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Schmidt, A.; Erdle, I.; Gamon, B.
Isolation and characterization of thiosulfate reductases from the green alga Chlorella fusca
Planta
162
243-249
1984
Chlorella fusca 211-8b, Chlorella fusca
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Akagi, J.M.; Drake, H.L.; Kim, J.H.; Gevertz, D.
Thiosulfate and trithionate reductases
Methods Enzymol.
243
260-270
1994
Desulfotomaculum nigrificans, Desulfovibrio vulgaris
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brenda
Bagchi, D.; Verma, D.
Partial purification and regulation of sulfur metabolizing enzymes in a cyanobacterium Phormidium uncinatum
Indian J. Exp. Biol.
35
876-880
1997
Phormidium uncinatum
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brenda
Bang, S.W.; Clark, D.S.; Keasling, J.D.
Cadmium, lead, and zinc removal by expression of the thiosulfate reductase gene from Salmonella typhimurium in Escherichia coli
Biotechnol. Lett.
22
1331-1335
2000
Salmonella enterica subsp. enterica serovar Typhimurium
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brenda
Sugio, T.; Kishimoto, K.; Oda, K.
Thiosulfate reductase from a moderately thermophilic iron-oxidizing bacterium, strain TI-1: purification and characterization
Biosci. Biotechnol. Biochem.
61
470-474
1997
thermophilic iron-oxidizing bacterium strain TI-1
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brenda
Stoffels, L.; Krehenbrink, M.; Berks, B.C.; Unden, G.
Thiosulfate reduction in Salmonella enterica is driven by the proton motive force
J. Bacteriol.
194
475-485
2012
Salmonella enterica
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Wang, Z.; Wang, G.; Xiang, Q.; Zhang, Y.; Wang, H.
Identification and characterization of a multi-domain sulfurtransferase in Phanerochaete chrysosporium
Biotechnol. Lett.
36
993-999
2014
Phanerochaete chrysosporium (I3RJK8)
brenda
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