Application | Comment | Organism |
---|---|---|
medicine | CysA2 interacts with pulmonary cells, and it is capable to activate macrophages and dendritic cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
structure of CysA2 at 2.29 A shows two alpha/beta domains. The N domain is inactive, whereas the C domain is responsible for the catalytic activity. The conserved catalytic cysteine of the active site is Cys233 | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.66 | - |
cyanide | pH 8.0, 37°C | Mycobacterium tuberculosis | |
7.02 | - |
3-Mercaptopyruvate | pH 8.0, 37°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
gel filtratioin | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHF9 | cf. EC 2.8.1.1 | - |
Mycobacterium tuberculosis H37Rv | P9WHF9 | cf. EC 2.8.1.1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + cyanide | - |
Mycobacterium tuberculosis | pyruvate + thiocyanate | - |
? | |
3-mercaptopyruvate + cyanide | - |
Mycobacterium tuberculosis H37Rv | pyruvate + thiocyanate | - |
? | |
thiosulfate + cyanide | - |
Mycobacterium tuberculosis | sulfite + thiocyanate | - |
? | |
thiosulfate + cyanide | - |
Mycobacterium tuberculosis H37Rv | sulfite + thiocyanate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31015, calculated from sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
CysA2 | - |
Mycobacterium tuberculosis |
Rv0815 | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58.3 | - |
melting temperature | Mycobacterium tuberculosis |