Information on EC 2.8.1.2 - 3-mercaptopyruvate sulfurtransferase

3-mercaptopyruvate + 2-mercaptoethanol

?

-

-

?

3-mercaptopyruvate + 2-mercaptoethanol

pyruvate + ?

-

-

?

3-mercaptopyruvate + cyanide

H2S + ?

H2S is produced by 3-mercaptopyruvate sulfurtransferase with cysteine aminotransferase in the presence of cysteine and alpha-ketoglutarate, supporting the existence of 3-mercaptopyruvate which has not been identified. Mercaptopyruvate can be provided by the metabolism of cysteine and alpha-ketoglutarate by cysteine aminotransferase (CAT)

690476

a major source of H2S production in the brain is from the enzyme 3-mercaptopyruvate sulfurtransferase

-

?

3-mercaptopyruvate + cyanide

pyruvate + thiocyanate

20 entries

3-mercaptopyruvate + dihydrolipoic acid

?

-

-

?

3-mercaptopyruvate + dithiothreitol

?

3-mercaptopyruvate + glutathione

?

-

-

?

3-mercaptopyruvate + HSO3-

pyruvate + S2O3-

-

725310

-

?

3-mercaptopyruvate + HSO3-

pyruvate + S2O32-

-

738198

-

?

3-mercaptopyruvate + L-cysteine

?

-

-

?

3-mercaptopyruvate + L-homocysteine

?

-

-

?

3-mercaptopyruvate + thioredoxin

?

thioredoxin is the preferred persulfide acceptor

-

?

3-mercaptopyruvate + thioredoxin

pyruvate + persulfurated thioredoxin

-

-

?

3-mercaptopyruvate + thiosulfate

pyruvate + H2S

-

724231

-

?

cysteine + 2-mercaptoethanol

? + ?

-

-

?

sulfane sulfur + cyanide

thiocyanate + ?

thioredoxin + 2-mercaptoethanol

thioredoxin persulfide + ?

-

-

?

thiosulfate + 2-mercaptoethanol

sulfate + ?

-

-

?

thiosulfate + cyanide

SO32- + thiocyanate

-

-

?

thiosulfate + thioredoxin

?

additional information

?

-

17 entries

3-mercaptopyruvate + cyanide

H2S + ?

H2S is produced by 3-mercaptopyruvate sulfurtransferase with cysteine aminotransferase in the presence of cysteine and alpha-ketoglutarate, supporting the existence of 3-mercaptopyruvate which has not been identified. Mercaptopyruvate can be provided by the metabolism of cysteine and alpha-ketoglutarate by cysteine aminotransferase (CAT)

690476

a major source of H2S production in the brain is from the enzyme 3-mercaptopyruvate sulfurtransferase

-

?

3-mercaptopyruvate + cyanide

pyruvate + thiocyanate

17 entries

3-mercaptopyruvate + HSO3-

pyruvate + S2O32-

-

738198

-

?

3-mercaptopyruvate + thioredoxin

pyruvate + persulfurated thioredoxin

-

-

?

thiosulfate + cyanide

SO32- + thiocyanate

-

-

?

additional information

?

-

11 entries

Cu2+

K2SO4

-

0.02 M, 70% activation

KCl

-

0.02 M, 70% activation

Na2SO4

-

0.02 M, 70% activation

Zn2+

additional information

2-mercaptoethanol

-

high concentrations

2-mercaptopropionic acid

-

uncompetitive inhibitor with respect to 3-mercaptopyruvate

2-oxobutyrate

uncompetitive inhibition

739470

3-Chloropyruvate

-

one-on-one manner of inactivation, pseudo first-order kinetics

661282

3-Mercaptopropionic acid

-

non-competitive inhibitor

alpha-Ketobutyrate

-

uncompetitive inhibitor with respect to 3-mercaptopyruvate, 50% inhibition at 13.7 mM

alpha-ketoglutarate

-

uncompetitive inhibitor with respect to 3-mercaptopyruvate, 50% inhibition at 9.5 mM

cyanide

-

inhibits at short-time intervals, slight enhancement at longer periods

cysteamine

-

slight

cysteine

-

dithiothreitol

-

inhibits from 3 mM to 4 mM at a 3-mercaptopyruvate concentration of 15 mM

ethylene

Arabidopsis thaliana

-

fumigation with ethylene decreases activity to less than 50%

glutathione

-

hydrogen peroxide

iodoacetate

menadione

-

1 h incubation at 0.02 mM leads to loss of the activity of 3-mercaptosulfurtransferase by 20%. In addition, thiosulfate sulfurtransferase and the level of sulfane sulfur and glutathione are decreased

671035

Mercaptosuccinamic acid

-

slight

N-alpha-4-tosyl-L-lysine chloromethyl ketone

-

interferes with Ser-255, 50% inhibition at 0.044 mM

645558

phenylmethanesulfonyl chloride

-

50% inhibition at 0.7 mM

645558

pyruvate

sulfite

-

H2S production by the enzyme is suppressed by sulfite

724231

tetrathionate

4 entries

thioglycolic acid

-

slight

1,4-dithiothreitol

-

diallyl disulfide

-

increase in activity of 3-mercaptosulfotransferase and gamma-cystathionase and elevation of hepatic sulfane sulfur level after intraperitoneal treatment with diallyl disulfie. In Ehrlich ascites cells, diallyl disulfide does not enzymic activites or sulfane sulfur level

673461

dithiothreitol

thioredoxin

6 entries

additional information

-

19 - 155

2-mercaptoethanol

0.02 - 73

3-Mercaptopyruvate

29 entries

4.45 - 6

cyanide

8.2

cysteine

-

pH 8.4, 30°C

4.4

dihydrolipoic acid

in 200 mM of HEPES, pH 7.4, at 37°C

2.59 - 4.6

dithiothreitol

28

glutathione

in 200 mM of HEPES, pH 7.4, at 37°C

4.1

L-cysteine

in 200 mM of HEPES, pH 7.4, at 37°C

12.5

L-homocysteine

in 200 mM of HEPES, pH 7.4, at 37°C

0.3

reduced thioredoxin

Q7K9G0

pH 7.3, 37°C

645569

0.0025 - 0.0028

thioredoxin

1.7 - 266

thiosulfate

229 - 5310

2-mercaptoethanol

0.0783 - 2260

3-Mercaptopyruvate

15 entries

2.4

cyanide

in 200 mM of HEPES, pH 7.4, at 37°C

2240

cysteine

-

pH 8.4, 30°C

1.7

dihydrolipoic acid

in 200 mM of HEPES, pH 7.4, at 37°C

6.1

dithiothreitol

in 200 mM of HEPES, pH 7.4, at 37°C

0.3

glutathione

in 200 mM of HEPES, pH 7.4, at 37°C

1.1

L-cysteine

in 200 mM of HEPES, pH 7.4, at 37°C

0.8

L-homocysteine

in 200 mM of HEPES, pH 7.4, at 37°C

1.3 - 172

thioredoxin

11.3

thiosulfate

-

pH 8.4, 30°C

2.1 - 390

2-mercaptoethanol

6.6 - 35000

3-Mercaptopyruvate

5 entries

0.4 - 2

cyanide

270

cysteine

-

pH 8.4, 30°C

0.39

dihydrolipoic acid

in 200 mM of HEPES, pH 7.4, at 37°C

1.3 - 7.8

dithiothreitol

0.01

glutathione

in 200 mM of HEPES, pH 7.4, at 37°C

0.27

L-cysteine

in 200 mM of HEPES, pH 7.4, at 37°C

0.06

L-homocysteine

in 200 mM of HEPES, pH 7.4, at 37°C

520 - 62000

thioredoxin

0.0427

thiosulfate

-

pH 8.4, 30°C

3.74 - 20

3-Chloropyruvate

17

3-Mercaptopropionic acid

-

pH 8, 30°C

6.1

alpha-Ketobutyrate

-

pH 8, 30°C

6.1

alpha-ketoglutarate

-

pH 8, 30°C

0.12

hydrogen peroxide

-

pH 7.4, 0°C

662415

6.4

pyruvate

-

pH 8, 30°C

0.178

tetrathionate

-

pH 7.4, 0°C

662415

1.74

-

liver, cytosolic fraction

1240

-

232

-

liver enzyme

24.27

-

mitochondrial fraction

334

-

3793

-

498

-

erythrocyte enzyme

5.26

-

liver, mitochondrial fraction

645561

540

-

additional information

-

10.5

-

6.9 - 7.6

8.2

-

9.3 - 9.6

-

additional information

-

30

45 - 50

-

49

-

45 - 60

-

45-50°C: temperature optimum, 60°C: no activity

5.1

-

two or three forms

645551

5.5

-

four enzymically active forms

-

-

-

645552, 645553, 645556, 645557, 645566, 645567

-

-

SwissProt

-

-

Rattus norvegicus Wistar

Wistar

645554, 645564

-

Ehrlich ascites carcinoma cell

-

-

-

-

-

-

3MST and cytosolic and mitochondrial cysteine aminotransferases are localized to endothelial cells of the thoracic aorta

-

-

-

-

-

-

-

-

-

-

-

-

highest expression

-

-

-

-

-

-

-

-

-

malfunction

metabolism

physiological function

5 entries

1urh, download, 3D-view

1okg, download, 3D-view

10900

-

mitochondria, gel filtration

645498

23800

-

sedimentation equilibrium ultracentrifugation

25000

x * 25000, SDS-PAGE

30000

x * 30000, SDS-PAGE

30200

-

cytosol, gel filtration

645498

32000

32700

-

x * 32700, calculated, x * 33000, SDS-PAGE

32800

33000

33000 - 34000

-

gel filtration

645551

34000

34500

-

two peaks with molecular masses of 34500 Da and 53500 Da, enzyme exists as a monomer and homodimer, gel filtration

36000

40000

50000

-

gel filtration

53000

-

two peaks with molecular masses of 34500 Da and 53500 Da, enzyme exists as a monomer and homodimer, gel filtration

645564

60000

-

gel filtration

66600

?

dimer

homodimer

monomer

sialoprotein

-

219 amino acids and 38 carbohydrate residues

645550

additional information

-

redox state controls enzyme activity

-

sitting drop vapor diffusion method, using 2 M ammonium sulfate, 0.1 M sodium acetate pH 4.5 and 0.02 M betaine hydrochloride

hanging drop vapor diffusion method

-

645558

C154S

-

active site mutant

723958

C247S

C263S

-

active site mutant

R187G

R196G

C154S

C247S

C248R

slightly increased Km

C254S

C263S

C64S

R178G

increased Km

R187G

-

Ki-value for 3-chloropyruvate similar to wild-type

R196G

R248G

facilitated catalysis of thiosulfate

645563

S249A

no significant difference in Km compared to wild-type enzyme

645563

S249K

additional information

4 entries

dialysis against urea inactivates, effect is reversed by dialysis, dilution or electrophoresis

-

markedly stabilized during purification and storage by the presence of monovalent cations, maximal stability is obtained if purification and storage are carried out at pH 6.5-7.5 in presence of 0.8 M KCl and 2 mM 2-mercaptoethanol

-

stabilized in 0.8 M KCl

-

unstable to thawing and refreezing

-

very unstable, spontanous inactivation can be partly prevented by glycerol

-

645550

oxidative stress (0.1-0.5 mM H2O2) inhibits enzyme activity

-

724194

-20°C, stable for at least 12 months without loss of activity

-35°C, 50 mM potassium phosphate buffer, pH 7.4, 0.5 mg/ml bovine serum albumin, 50% glycerol, slow decrease of activity

-

645556

4°C, no loss of activity after 10 days

-

4°C, several days

-

-

glutathione-agarose column chromatography and benzamidine Sepharose column chromatography

738634

GST Spin Trap column chromatography

739661

GSTrap FF affinity column chromatography

-

724194

homogeneity

Ni-NTA agarose column chromatography and DEAE-Sepharose column chromatography

partial

wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus

-

AtMST1 and AtST2

expressed in COS cells and Escherichia coli BL21 cells

739661

expressed in Escherichia coli BL21(DE3) cells

expressed in Escherichia coli BL21(DE3) Codon Plus cells

-

724194

expression in Escherichia coli BL21 (DE3)

liver enzyme

-

transient transfection of human embryonic kidney HEK 293-F cells

690476

wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus

Q7K9G0

645569

following permanent middle cerebral artery occlusion, the enzyme is down-regulated in both the cortex and striatum, but not in the corpus collosum

739470

the hepatic and renal enzyme activity increases by 0.5 LD50 potassium cyanide. Elevated renal cyanide level is also accompanied by increased enzyme activity

738551

enzyme inhibited by hydrogen peroxide together with stoichiometric concentration of tetrathionate, complete renaturation by dithiothreitol or thioredoxin