Information on EC 2.8.1.2 - 3-mercaptopyruvate sulfurtransferase

3-mercaptopyruvate sulphurtransferase

3-mercaptopyruvate:cyanide sulfurtransferase

-

3-MPST

3-MST

-

3MST

beta-mercaptopyruvate sulfurtransferase

-

beta-mercaptopyruvate trans-sulfurase

-

mercaptopyruvate sulfurtransferase

MPST

MST

rhodanese

-

SseA

-

sulfurtransferase, 3-mercaptopyruvate

-

TUM1

-

738634

additional information

Cysteine and methionine metabolism

-

rhodanese activity, EC 2.8.1.1 is a minor function of human erythrocyte beta-mercaptopyruvate sulfurtransferase

645495

3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin

6 entries

3-mercaptopyruvate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine = pyruvate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine

(1a)

-

[3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + reduced thioredoxin = hydrogen sulfide + [3-mercaptopyruvate sulfurtransferase]-L-cysteine + oxidized thioredoxin

(1b)

-

sulfur atom transfer

-

BRENDA

cysteine metabolism

KEGG

MetaCyc

L-cysteine degradation III

3-mercaptopyruvate:cyanide sulfurtransferase

The enzyme catalyses a transsulfuration reaction from 3-mercaptopyruvate to an internal cysteine residue. In the presence of a dithiol such as reduced thioredoxin or dihydrolipoate, the sulfanyl sulfur is released as hydrogen sulfide. The enzyme participates in a sulfur relay process that leads to the 2-thiolation of some tRNAs and to protein urmylation by transferring sulfur between the NFS1 cysteine desulfurase (EC 2.8.1.7) and the MOCS3 sulfurtransferase (EC 2.8.1.11).

9026-05-5

-

2-mercaptoethanol + cyanide

ethanol + thiocyanate

3-mercaptopyruvate + 2-mercaptoethanol

?

-

-

?

3-mercaptopyruvate + 2-mercaptoethanol

pyruvate + ?

-

-

?

3-mercaptopyruvate + cyanide

H2S + ?

Q99J99

H2S is produced by 3-mercaptopyruvate sulfurtransferase with cysteine aminotransferase in the presence of cysteine and alpha-ketoglutarate, supporting the existence of 3-mercaptopyruvate which has not been identified. Mercaptopyruvate can be provided by the metabolism of cysteine and alpha-ketoglutarate by cysteine aminotransferase (CAT)

690476

a major source of H2S production in the brain is from the enzyme 3-mercaptopyruvate sulfurtransferase

-

?

3-mercaptopyruvate + cyanide

pyruvate + thiocyanate

20 entries

3-mercaptopyruvate + dihydrolipoic acid

?

-

-

?

3-mercaptopyruvate + dithiothreitol

?

3-mercaptopyruvate + glutathione

?

-

-

?

3-mercaptopyruvate + HSO3-

pyruvate + S2O3-

-

725310

-

?

3-mercaptopyruvate + HSO3-

pyruvate + S2O32-

Q99J99

-

738198

-

?

3-mercaptopyruvate + L-cysteine

?

-

-

?

3-mercaptopyruvate + L-homocysteine

?

-

-

?

3-mercaptopyruvate + thioredoxin

?

thioredoxin is the preferred persulfide acceptor

-

?

3-mercaptopyruvate + thioredoxin

pyruvate + persulfurated thioredoxin

-

-

?

3-mercaptopyruvate + thiosulfate

pyruvate + H2S

-

724231

-

?

cysteine + 2-mercaptoethanol

? + ?

-

-

?

sulfane sulfur + cyanide

thiocyanate + ?

thioredoxin + 2-mercaptoethanol

thioredoxin persulfide + ?

-

-

?

thiosulfate + 2-mercaptoethanol

sulfate + ?

-

-

?

thiosulfate + cyanide

SO32- + thiocyanate

-

-

?

thiosulfate + thioredoxin

?

additional information

?

-

16 entries

3-mercaptopyruvate + cyanide

H2S + ?

Q99J99

H2S is produced by 3-mercaptopyruvate sulfurtransferase with cysteine aminotransferase in the presence of cysteine and alpha-ketoglutarate, supporting the existence of 3-mercaptopyruvate which has not been identified. Mercaptopyruvate can be provided by the metabolism of cysteine and alpha-ketoglutarate by cysteine aminotransferase (CAT)

690476

a major source of H2S production in the brain is from the enzyme 3-mercaptopyruvate sulfurtransferase

-

?

3-mercaptopyruvate + cyanide

pyruvate + thiocyanate

17 entries

3-mercaptopyruvate + HSO3-

pyruvate + S2O32-

Q99J99

-

738198

-

?

3-mercaptopyruvate + thioredoxin

pyruvate + persulfurated thioredoxin

-

-

?

thiosulfate + cyanide

SO32- + thiocyanate

-

-

?

additional information

?

-

10 entries

Cu2+

K2SO4

-

0.02 M, 70% activation

KCl

-

0.02 M, 70% activation

Na2SO4

-

0.02 M, 70% activation

Zn2+

additional information

2-mercaptoethanol

-

high concentrations

2-mercaptopropionic acid

-

uncompetitive inhibitor with respect to 3-mercaptopyruvate

2-oxobutyrate

-

uncompetitive inhibition

739470

3-Chloropyruvate

-

one-on-one manner of inactivation, pseudo first-order kinetics

661282

3-Mercaptopropionic acid

-

non-competitive inhibitor

alpha-Ketobutyrate

-

uncompetitive inhibitor with respect to 3-mercaptopyruvate, 50% inhibition at 13.7 mM

alpha-ketoglutarate

-

uncompetitive inhibitor with respect to 3-mercaptopyruvate, 50% inhibition at 9.5 mM

cyanide

-

inhibits at short-time intervals, slight enhancement at longer periods

cysteamine

-

slight

cysteine

-

dithiothreitol

-

inhibits from 3 mM to 4 mM at a 3-mercaptopyruvate concentration of 15 mM

ethylene

Arabidopsis thaliana

-

fumigation with ethylene decreases activity to less than 50%

glutathione

-

hydrogen peroxide

iodoacetate

menadione

-

1 h incubation at 0.02 mM leads to loss of the activity of 3-mercaptosulfurtransferase by 20%. In addition, thiosulfate sulfurtransferase and the level of sulfane sulfur and glutathione are decreased

671035

Mercaptosuccinamic acid

-

slight

N-alpha-4-tosyl-L-lysine chloromethyl ketone

-

interferes with Ser-255, 50% inhibition at 0.044 mM

645558

phenylmethanesulfonyl chloride

-

50% inhibition at 0.7 mM

645558

pyruvate

sulfite

-

H2S production by the enzyme is suppressed by sulfite

724231

tetrathionate

thioglycolic acid

-

slight

1,4-dithiothreitol

-

diallyl disulfide

-

increase in activity of 3-mercaptosulfotransferase and gamma-cystathionase and elevation of hepatic sulfane sulfur level after intraperitoneal treatment with diallyl disulfie. In Ehrlich ascites cells, diallyl disulfide does not enzymic activites or sulfane sulfur level

673461

dithiothreitol

thioredoxin

additional information

-

reduced glutathione does not affect MST activity

674808, 694056

-

19 - 155

2-mercaptoethanol

0.02 - 73

3-Mercaptopyruvate

28 entries

4.45 - 6

cyanide

8.2

cysteine

-

pH 8.4, 30°C

4.4

dihydrolipoic acid

-

in 200 mM of HEPES, pH 7.4, at 37°C

2.59 - 4.6

dithiothreitol

28

glutathione

-

in 200 mM of HEPES, pH 7.4, at 37°C

4.1

L-cysteine

-

in 200 mM of HEPES, pH 7.4, at 37°C

12.5

L-homocysteine

-

in 200 mM of HEPES, pH 7.4, at 37°C

0.3

reduced thioredoxin

Q7K9G0

pH 7.3, 37°C

645569

0.0025 - 0.0028

thioredoxin

1.7 - 266

thiosulfate

229 - 5310

2-mercaptoethanol

0.0783 - 2260

3-Mercaptopyruvate

15 entries

2.4

cyanide

-

in 200 mM of HEPES, pH 7.4, at 37°C

2240

cysteine

-

pH 8.4, 30°C

1.7

dihydrolipoic acid

-

in 200 mM of HEPES, pH 7.4, at 37°C

6.1

dithiothreitol

-

in 200 mM of HEPES, pH 7.4, at 37°C

0.3

glutathione

-

in 200 mM of HEPES, pH 7.4, at 37°C

1.1

L-cysteine

-

in 200 mM of HEPES, pH 7.4, at 37°C

0.8

L-homocysteine

-

in 200 mM of HEPES, pH 7.4, at 37°C

1.3 - 172

thioredoxin

11.3

thiosulfate

-

pH 8.4, 30°C

2.1 - 390

2-mercaptoethanol

6.6 - 35000

3-Mercaptopyruvate

0.4 - 2

cyanide

270

cysteine

-

pH 8.4, 30°C

0.39

dihydrolipoic acid

-

in 200 mM of HEPES, pH 7.4, at 37°C

1.3 - 7.8

dithiothreitol

0.01

glutathione

-

in 200 mM of HEPES, pH 7.4, at 37°C

0.27

L-cysteine

-

in 200 mM of HEPES, pH 7.4, at 37°C

0.06

L-homocysteine

-

in 200 mM of HEPES, pH 7.4, at 37°C

520 - 62000

thioredoxin

0.0427

thiosulfate

-

pH 8.4, 30°C

3.74 - 20

3-Chloropyruvate

17

3-Mercaptopropionic acid

-

pH 8, 30°C

6.1

alpha-Ketobutyrate

-

pH 8, 30°C

6.1

alpha-ketoglutarate

-

pH 8, 30°C

0.12

hydrogen peroxide

-

pH 7.4, 0°C

662415

6.4

pyruvate

-

pH 8, 30°C

0.178

tetrathionate

-

pH 7.4, 0°C

662415

1.74

-

liver, cytosolic fraction

645561

5.26

-

liver, mitochondrial fraction

645561

24.27

-

mitochondrial fraction

645554

232

-

liver enzyme

334

-

498

-

erythrocyte enzyme

540

-

1240

-

3793

-

additional information

-

6.9 - 7.6

8.2

-

9.3 - 9.6

-

10.5

-

additional information

-

30

45 - 50

-

49

-

45 - 60

-

45-50°C: temperature optimum, 60°C: no activity

5.1

-

two or three forms

645551

5.5

-

four enzymically active forms

-

-

-

645552, 645553, 645556, 645557, 645566, 645567

-

-

SwissProt

-

-

Rattus norvegicus Wistar

Wistar

645554, 645564

-

-

-

-

-

Q7K9G0

-

645569

aorta

Ehrlich ascites carcinoma cell

-

3MST and cytosolic and mitochondrial cysteine aminotransferases are localized to endothelial cells of the thoracic aorta

-

-

-

-

-

-

-

-

645561, 645562, 738988

-

-

-

-

-

highest expression

-

-

-

-

-

-

-

-

-

malfunction

metabolism

physiological function

5 entries

1urh, download, 3D-view

SCOPe (1urh)

CATH (1urh)

P31142

Escherichia coli (strain K12)

1okg, download, 3D-view

Q7K9G0

10900

-

mitochondria, gel filtration

645498

23800

-

sedimentation equilibrium ultracentrifugation

25000

-

x * 25000, SDS-PAGE

738634

30000

-

x * 30000, SDS-PAGE

30200

Rana temporaria

-

cytosol, gel filtration

645498

32000

32700

-

x * 32700, calculated, x * 33000, SDS-PAGE

32800

33000 - 34000

-

gel filtration

645551

33000

5 entries

34000

-

1 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration; 2 * 34000, enzyme exists as a monomer and homodimer, SDS-PAGE, gel filtration

645564

34500

-

two peaks with molecular masses of 34500 Da and 53500 Da, enzyme exists as a monomer and homodimer, gel filtration

36000

40000

50000

-

gel filtration

645547

53000

-

two peaks with molecular masses of 34500 Da and 53500 Da, enzyme exists as a monomer and homodimer, gel filtration

645564

60000

-

gel filtration

66600

?

dimer

homodimer

monomer

sialoprotein

-

219 amino acids and 38 carbohydrate residues

645550

additional information

-

redox state controls enzyme activity

-

sitting drop vapor diffusion method, using 2 M ammonium sulfate, 0.1 M sodium acetate pH 4.5 and 0.02 M betaine hydrochloride

-

hanging drop vapor diffusion method

-

645558

C154S

-

active site mutant

723958

C247S

C263S

-

active site mutant

R187G

R196G

C154S

C247S

C248R

-

slightly increased Km

C254S

C263S

C64S

R178G

-

increased Km

645563

R187G

-

Ki-value for 3-chloropyruvate similar to wild-type

661282

R196G

R248G

-

facilitated catalysis of thiosulfate

645563

S249A

-

no significant difference in Km compared to wild-type enzyme

645563

S249K

additional information

dialysis against urea inactivates, effect is reversed by dialysis, dilution or electrophoresis

-

markedly stabilized during purification and storage by the presence of monovalent cations, maximal stability is obtained if purification and storage are carried out at pH 6.5-7.5 in presence of 0.8 M KCl and 2 mM 2-mercaptoethanol

-

stabilized in 0.8 M KCl

-

unstable to thawing and refreezing

-

645547

very unstable, spontanous inactivation can be partly prevented by glycerol

-

645550

oxidative stress (0.1-0.5 mM H2O2) inhibits enzyme activity

-

724194

-20°C, stable for at least 12 months without loss of activity

-35°C, 50 mM potassium phosphate buffer, pH 7.4, 0.5 mg/ml bovine serum albumin, 50% glycerol, slow decrease of activity

-

645556

4°C, no loss of activity after 10 days

-

4°C, several days

-

-

glutathione-agarose column chromatography and benzamidine Sepharose column chromatography

-

738634

GST Spin Trap column chromatography

-

739661

GSTrap FF affinity column chromatography

-

724194

homogeneity

Ni-NTA agarose column chromatography and DEAE-Sepharose column chromatography

-

partial

wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus

Q7K9G0

-

AtMST1 and AtST2

expressed in COS cells and Escherichia coli BL21 cells

-

739661

expressed in Escherichia coli BL21(DE3) cells

725528, 738634

expressed in Escherichia coli BL21(DE3) Codon Plus cells

-

724194

expression in Escherichia coli BL21 (DE3)

liver enzyme

-

transient transfection of human embryonic kidney HEK 293-F cells

Q99J99

690476

wild-type and 4 truncated versions lacking 10, 32, 50 and 70 amino acids at the C-terminus

Q7K9G0

645569

following permanent middle cerebral artery occlusion, the enzyme is down-regulated in both the cortex and striatum, but not in the corpus collosum

-

739470

the hepatic and renal enzyme activity increases by 0.5 LD50 potassium cyanide. Elevated renal cyanide level is also accompanied by increased enzyme activity

-

738551

enzyme inhibited by hydrogen peroxide together with stoichiometric concentration of tetrathionate, complete renaturation by dithiothreitol or thioredoxin