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3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin
3-mercaptopyruvate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine = pyruvate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
(1a)
-
-
-
[3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + reduced thioredoxin = hydrogen sulfide + [3-mercaptopyruvate sulfurtransferase]-L-cysteine + oxidized thioredoxin
(1b)
-
-
-
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin

mechanism
-
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin
mechanism
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin
rapid equilibrium-ordered mechanism with 3-mercaptopyruvate as the first substrate
-
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin
sequential formal mechanism
-
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin
when 2-mercaptoethanol is the sulfur acceptor addition of the substrate is random
-
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin
rapid equilibrium-ordered mechanism
-
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin
sulfur transfer mechanism involving C237 as the nucleophilic species and H66, R102 and D262 as residues assisting catalysis
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin
-
-
-
-
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2-mercaptoethanol + cyanide
ethanol + thiocyanate
2-oxo-3-sulfanylpropanoate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine
pyruvate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
-
-
-
?
3-mercaptopyruvate + 2 glutathione
pyruvate + glutathione disulfide
-
-
-
?
3-mercaptopyruvate + 2-mercaptoethanol
?
-
-
-
?
3-mercaptopyruvate + 2-mercaptoethanol
pyruvate + ?
-
-
-
-
?
3-mercaptopyruvate + cyanide
H2S + ?
H2S is produced by 3-mercaptopyruvate sulfurtransferase with cysteine aminotransferase in the presence of cysteine and alpha-ketoglutarate, supporting the existence of 3-mercaptopyruvate which has not been identified. Mercaptopyruvate can be provided by the metabolism of cysteine and alpha-ketoglutarate by cysteine aminotransferase (CAT)
a major source of H2S production in the brain is from the enzyme 3-mercaptopyruvate sulfurtransferase
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
3-mercaptopyruvate + dihydrolipoic acid
?
-
-
-
?
3-mercaptopyruvate + dithiothreitol
?
3-mercaptopyruvate + glutathione
?
-
-
-
?
3-mercaptopyruvate + HSO3-
pyruvate + S2O3-
-
-
-
-
?
3-mercaptopyruvate + HSO3-
pyruvate + S2O32-
-
-
-
?
3-mercaptopyruvate + L-cysteine
?
3-mercaptopyruvate + L-homocysteine
?
-
-
-
?
3-mercaptopyruvate + N-acetyl-L-cysteine
?
3-mercaptopyruvate + thioredoxin
?
thioredoxin is the preferred persulfide acceptor
-
-
?
3-mercaptopyruvate + thioredoxin
pyruvate + persulfurated thioredoxin
3-mercaptopyruvate + thiosulfate
pyruvate + H2S
-
-
-
-
?
7-azido-4-methylcoumarin + L-cysteine
?
-
-
-
?
7-azido-4-methylcoumarin + N-acetyl-L-cysteine
?
-
-
-
?
cysteine + 2-mercaptoethanol
? + ?
-
-
-
-
?
sulfane sulfur + cyanide
thiocyanate + ?
thioredoxin + 2-mercaptoethanol
thioredoxin persulfide + ?
-
-
-
-
?
thiosulfate + 2-mercaptoethanol
sulfate + ?
-
-
-
-
?
thiosulfate + cyanide
SO32- + thiocyanate
-
-
-
?
thiosulfate + cyanide
sulfite + thiocyanate
thiosulfate + glutathione
sulfite + glutathione disulfide
-
-
-
?
thiosulfate + thioredoxin
?
thiosulfate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine
sulfate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
[3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + reduced thioredoxin
hydrogen sulfide + [3-mercaptopyruvate sulfurtransferase]-L-cysteine + oxidized thioredoxin
additional information
?
-
2-mercaptoethanol + cyanide

ethanol + thiocyanate
-
-
-
-
?
2-mercaptoethanol + cyanide
ethanol + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide

pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + dithiothreitol

?
-
-
-
?
3-mercaptopyruvate + dithiothreitol
?
-
-
-
-
?
3-mercaptopyruvate + dithiothreitol
?
-
-
-
?
3-mercaptopyruvate + L-cysteine

?
-
-
-
?
3-mercaptopyruvate + L-cysteine
?
-
-
-
-
?
3-mercaptopyruvate + L-cysteine
?
-
-
-
?
3-mercaptopyruvate + N-acetyl-L-cysteine

?
-
-
-
?
3-mercaptopyruvate + N-acetyl-L-cysteine
?
-
-
-
?
3-mercaptopyruvate + thioredoxin

pyruvate + persulfurated thioredoxin
-
-
-
-
?
3-mercaptopyruvate + thioredoxin
pyruvate + persulfurated thioredoxin
-
-
-
-
?
3-mercaptopyruvate + thioredoxin
pyruvate + persulfurated thioredoxin
-
-
-
?
3-mercaptopyruvate + thioredoxin
pyruvate + persulfurated thioredoxin
-
-
-
-
?
3-mercaptopyruvate + thioredoxin
pyruvate + persulfurated thioredoxin
-
-
-
?
3-mercaptopyruvate + thioredoxin
pyruvate + persulfurated thioredoxin
-
-
-
?
sulfane sulfur + cyanide

thiocyanate + ?
-
-
?
sulfane sulfur + cyanide
thiocyanate + ?
-
-
?
thiosulfate + cyanide

sulfite + thiocyanate
-
-
-
?
thiosulfate + cyanide
sulfite + thiocyanate
-
-
-
?
thiosulfate + thioredoxin

?
-
-
-
?
thiosulfate + thioredoxin
?
-
-
-
?
thiosulfate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine

sulfate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
-
-
-
?
thiosulfate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine
sulfate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
-
-
-
?
[3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + reduced thioredoxin

hydrogen sulfide + [3-mercaptopyruvate sulfurtransferase]-L-cysteine + oxidized thioredoxin
-
-
-
?
[3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + reduced thioredoxin
hydrogen sulfide + [3-mercaptopyruvate sulfurtransferase]-L-cysteine + oxidized thioredoxin
-
-
-
?
additional information

?
-
-
the enzyme plays a role in iron-sulfur chromophore formation in adrenal cortex
-
-
?
additional information
?
-
-
cyanide detoxification
-
-
?
additional information
?
-
-
participates in L-cysteine desulfuration
-
-
?
additional information
?
-
-
3-mercaptopyruvate sulfurtransferase in conjunction with cysteine (aspartate) aminotransferase contributes significantly in generating H2S from L-cysteine in the presence of alpha-ketoglutarate
-
-
?
additional information
?
-
3-mercaptopyruvate sulfurtransferase produces bound sulfane sulfur
-
-
?
additional information
?
-
-
in the catalytic process of the enzyme, hydrogen peroxide is possibly produced by persulfide of the sulfur-accepted substrate and sulfur oxides are possibly produced in the redox cycle of persulfide formed at the catalytic site cysteine of the reaction intermediate
-
-
?
additional information
?
-
-
the enzyme produces hydrogen sulfide in the presence of both cysteine and 2-oxoglutarate. Thioredoxin and dihydrolipoic acid associate with 3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide. Other reducing substances, such as NADPH, NADH, GSH, cysteine and CoA, do not have any effect on the reaction
-
-
?
additional information
?
-
the enzyme 3MST also produces H2S2 and H2S5 from 3-mercaptopyruvate. H2S3 production from H2S occurs by the enzyme rhodanese
-
-
?
additional information
?
-
3MST produces Cys-SSH and GSSH together with the potential signaling molecules hydrogen per- and tri-sulfide (H2S2 and H2S3). Cys-SSH and GSSH are produced in the presence of physiological concentrations of cysteine and glutathione, while those with longer sulfur chains, Cys-SSnH and GSSnH, are produced in the presence of lower than physiological concentrations of cysteine and glutathione
-
-
-
additional information
?
-
-
role in metabolism of some amino acids and low molecular weight sulfur compounds
-
-
?
additional information
?
-
-
cyanide detoxification
-
-
?
additional information
?
-
-
MST contributes to maintain redox homeostasis
-
-
?
additional information
?
-
MST contributes to maintain redox homeostasis
-
-
?
additional information
?
-
MST contributes to maintain redox homeostasis via exerting control over cysteine catabolism
-
-
?
additional information
?
-
-
3MST and cytosolic and mitochondrial cysteine aminotransferases are localized to endothelial cells of the thoracic aorta and together enzymes produce H2S in this cell type. H2S is a smooth muscle relaxant released from endothelium
-
-
?
additional information
?
-
-
in the catalytic process of the enzyme, hydrogen peroxide is possibly produced by persulfide of the sulfur-accepted substrate and sulfur oxides are possibly produced in the redox cycle of persulfide formed at the catalytic site cysteine of the reaction intermediate
-
-
?
additional information
?
-
after prolonged incubation of MST with thiosulfate, a trisulfide adduct becomes predominant at the sulfurated catalytic-site cysteine. When these adducts are reduced by Trx with reducing system H2S2 first appears, and then H2S and H2S3
-
-
-
additional information
?
-
-
role in metabolism of some amino acids and low molecular weight sulfur compounds
-
-
?
additional information
?
-
-
has high activity with 3-MP as a sulfur donor and can use several thiol compounds as sulfur acceptor substrates
-
-
?
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3-mercaptopyruvate + cyanide
H2S + ?
H2S is produced by 3-mercaptopyruvate sulfurtransferase with cysteine aminotransferase in the presence of cysteine and alpha-ketoglutarate, supporting the existence of 3-mercaptopyruvate which has not been identified. Mercaptopyruvate can be provided by the metabolism of cysteine and alpha-ketoglutarate by cysteine aminotransferase (CAT)
a major source of H2S production in the brain is from the enzyme 3-mercaptopyruvate sulfurtransferase
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
3-mercaptopyruvate + HSO3-
pyruvate + S2O32-
-
-
-
?
3-mercaptopyruvate + thioredoxin
pyruvate + persulfurated thioredoxin
-
-
-
?
thiosulfate + cyanide
SO32- + thiocyanate
-
-
-
?
additional information
?
-
3-mercaptopyruvate + cyanide

pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
?
3-mercaptopyruvate + cyanide
pyruvate + thiocyanate
-
-
-
-
?
additional information

?
-
-
the enzyme plays a role in iron-sulfur chromophore formation in adrenal cortex
-
-
?
additional information
?
-
-
cyanide detoxification
-
-
?
additional information
?
-
-
participates in L-cysteine desulfuration
-
-
?
additional information
?
-
3-mercaptopyruvate sulfurtransferase produces bound sulfane sulfur
-
-
?
additional information
?
-
-
role in metabolism of some amino acids and low molecular weight sulfur compounds
-
-
?
additional information
?
-
-
cyanide detoxification
-
-
?
additional information
?
-
-
MST contributes to maintain redox homeostasis
-
-
?
additional information
?
-
MST contributes to maintain redox homeostasis
-
-
?
additional information
?
-
MST contributes to maintain redox homeostasis via exerting control over cysteine catabolism
-
-
?
additional information
?
-
-
3MST and cytosolic and mitochondrial cysteine aminotransferases are localized to endothelial cells of the thoracic aorta and together enzymes produce H2S in this cell type. H2S is a smooth muscle relaxant released from endothelium
-
-
?
additional information
?
-
-
role in metabolism of some amino acids and low molecular weight sulfur compounds
-
-
?
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1-(2,4-dihydroxyphenyl)-2-[(4-hydroxy-5,6-dimethylthieno[2,3-d]pyrimidin-2-yl)sulfanyl]ethan-1-one
more than 80% inhibition at 0.01 mM
-
2-mercaptoethanol
-
high concentrations
2-mercaptopropionic acid
-
uncompetitive inhibitor with respect to 3-mercaptopyruvate
2-oxobutyrate
uncompetitive inhibition
2-[2-[(4-oxo-3,4,4a,8a-tetrahydroquinazolin-2-yl)sulfanyl]acetamido]thiophene-3-carboxamide
more than 80% inhibition at 0.01 mM
2-[[2-(1,4-dihydronaphthalen-1-yl)-2-oxoethyl]sulfanyl]-6-methylpyrimidin-4(3H)-one
more than 80% inhibition at 0.01 mM, almost inactive towards cystathionine gamma-lyase and cystathionine beta-synthase, very low inhibiution of thiosulfate sulfurtransferase, Type II
3-Chloropyruvate
-
one-on-one manner of inactivation, pseudo first-order kinetics
3-Mercaptopropionic acid
-
non-competitive inhibitor
3-[(benzenesulfonyl)amino]benzoic acid
more than 80% inhibition at 0.01 mM
alpha-Ketobutyrate
-
uncompetitive inhibitor with respect to 3-mercaptopyruvate, 50% inhibition at 13.7 mM
alpha-ketoglutarate
-
uncompetitive inhibitor with respect to 3-mercaptopyruvate, 50% inhibition at 9.5 mM
cyanide
-
inhibits at short-time intervals, slight enhancement at longer periods
dithiothreitol
-
inhibits from 3 mM to 4 mM at a 3-mercaptopyruvate concentration of 15 mM
ethylene
-
fumigation with ethylene decreases activity to less than 50%
menadione
-
1 h incubation at 0.02 mM leads to loss of the activity of 3-mercaptosulfurtransferase by 20%. In addition, thiosulfate sulfurtransferase and the level of sulfane sulfur and glutathione are decreased
Mercaptosuccinamic acid
-
slight
N-alpha-4-tosyl-L-lysine chloromethyl ketone
-
interferes with Ser-255, 50% inhibition at 0.044 mM
phenylmethanesulfonyl chloride
-
50% inhibition at 0.7 mM
S-allyl-L-cysteine
significant decrease in MPST activity at 2245 microM after 24 h and 48 h incubation vs. 1000 microM is associated with decrease in sulfane sulfur levels
sulfite
-
H2S production by the enzyme is suppressed by sulfite
thioglycolic acid
-
slight
hydrogen peroxide

-
hydrogen peroxide (0.1-0.5 mM) causes a concentration-dependent decrease in the activity of recombinant enzyme (54% inhibition at 0.1 mM, 87% inhibition at 0.5 mM)
hydrogen peroxide
-
together with stoichiometric concentration of tetrathionate, renaturation by dithiothreitol or thioredoxin. Excess molar ratio of hydrogen peroxide inactivates
hydrogen peroxide
strong inhibition
iodoacetate

-
-
pyruvate

-
product inhibition, competitive with 3-mercaptopyruvate and 2-mercaptoethanol
pyruvate
-
uncompetitive inhibitor with respect to 3-mercaptopyruvate, 50% inhibition at 13.1 mM
pyruvate
-
10 mM: 17% inhibition, 20 mM: 45% inhibition
tetrathionate

-
reduced GSH and GSH together with the reducing system partially restore the activity of tetrathionate-inhibited enzyme. Enzyme that is oxidized by an excess molar dose of tetrathionate is completely reactivated by dithiothreitol, reduced thioredoxin, and thioredoxin together with the reducing system
tetrathionate
-
reduced GSH and GSH together with the reducing system partially restore the activity of tetrathionate-inhibited enzyme. Enzyme that is oxidized by an excess molar dose of tetrathionate is completely reactivated by dithiothreitol, reduced thioredoxin, and thioredoxin together with the reducing system
tetrathionate
strong inhibition
thioredoxin

substrate inhibition of mitochondrial splice variant MPST2
thioredoxin
substrate inhibition of mitochondrial splice variant MPST2
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